SuperSignal™ ELISA Pico Chemiluminescent Substrate, 100 mL Kit - FAQs

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8 product FAQs found

What type of instrument do I use to read the plates detected with the SuperSignal ELISA Pico Chemiluminescent Substrate?

A luminometer is used to measure relative light units.

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What microplates work best with the SuperSignal ELISA Pico Chemiluminescent Substrate?

White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal.

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What signal duration occurs with SuperSignal ELISA Pico Chemiluminescent Substrate?

Maximum signal is reached within five minutes and then declines slowly thereafter until it is much less after an hour.

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How sensitive is SuperSignal ELISA Pico Chemiluminescent Substrate?

The substrate allows measurement of low picogram levels in sandwich ELISA procedures.

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How does the SuperSignal ELISA Pico Chemiluminescent Substrate compare with QuantaBlu Fluorogenic Peroxidase Substrate?

QuantaBlu Fluorogenic Peroxidase Substrate is a fluorescent detection reagent for HRP and provides attamole sensitivity. SuperSignal ELISA Pico Chemiluminescent Substrate is strictly chemiluminescent.

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Can I dilute the SuperSignal ELISA Pico Chemiluminescent Substrate working reagent if my signal is too intense?

We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.

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At what wavelength is SuperSignal ELISA Pico Chemiluminescent Substrate signal measured?

The light emission wavelength is read at 425 nm.

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When using the SuperSignal ELISA Pico Chemiluminescent Substrate, why are the wells appearing red-orange-yellow in relation to the enzyme?

The substrate solution should remain colorless upon reaction with Horseradish peroxidase (HRP), if the HRP concentration is within the appropriate range. Extremely high concentrations of HRP can cause the substrate color to turn yellow/orange/brown. Alternatively, the color change of the substrate may be due to the presence of some other contaminant.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.