SuperSignal™ ELISA Femto Substrate

SuperSignal™ ELISA Femto Substrate - FAQs

2 个常见问题解答

Have you tested the SuperSignal ELISA Femto Substrate with magnetic beads?

We have not tested the SuperSignal ELISA Femto Substrate with magnetic beads. However, we know that some metals can interfere with the substrate. Therefore, depending on the chemistry of the beads, they may or may not interfere with the substrate.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Is the time taken to add your SuperSignal ELISA Femto Substrate to the wells of a microplate important?

Yes. The signal from the SuperSignal ELISA Femto Substrate ramps up very quickly and optimal signal production lasts only for 5-10 min. Therefore, we recommend using a multichannel pipette to add the substrate to the wells, mix for 1 min, and then read the signal produced. Typically, reading each well for 0.1-1 sec will provide good signal with this substrate.
Note: With the SuperSignal ELISA Pico Chemiluminescent Substrate (Cat. No. 37070, 37069), the signal does not decay as quickly and researchers typically have up to 30 min to read the plate.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.