Blocker™ Casein
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Blocker™ Casein
Thermo Scientific™

Blocker™ Casein

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.
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Catalog NumberQuantityChemical Name or Material
375321 LTBS
37582100 mLPBS
375281 LPBS
37583100 mLTBS
Catalog number 37532
Price (CNY)
2,319.00
Each
Add to cart
Quantity:
1 L
Chemical Name or Material:
TBS
Request bulk or custom format
Price (CNY)
2,319.00
Each
Add to cart
Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.
Features of Blocker Casein include:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialTBS
Recommended StorageStore at 4°C.
Concentration1X
For Use With (Application)Western Blot
Physical FormLiquid
Product LineBlocker™
Quantity1 L
Unit SizeEach

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the main ingredient in Blocker Casein (Cat. No. 37583, 37532)?

Blocker Casein (Cat. No. 37583, 37532) is a ready-to-use, 1% (w/v) solution of purified casein in Tris-buffer saline (TBS). It is a stable, thimerosal-free formulation and is already at the optimal concentration for most applications. However, it can be diluted further if needed.

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .