StartingBlock™ Blocking Buffer
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StartingBlock™ Blocking Buffer
Thermo Scientific™

StartingBlock™ Blocking Buffer

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays.
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Catalog NumberQuantityChemical Name or Material
375431 LTBST
37578100 mLPBS
375381 LPBS
37579100 mLTBS
375421 LTBS
375391 LPBST
Catalog number 37543
Price (CNY)
999.00
Online Exclusive
Ends: 31-Dec-2025
3,470.00
Save 2,471.00 (71%)
Each
Add to cart
Quantity:
1 L
Chemical Name or Material:
TBST
Request bulk or custom format
Price (CNY)
999.00
Online Exclusive
Ends: 31-Dec-2025
3,470.00
Save 2,471.00 (71%)
Each
Add to cart
StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock Blocking Buffer is available in PBS and TBS, with and without Tween 20.
Features of StartingBlock Blocking Buffer include:
• Strip and re-probe without re-blocking—blots stay blocked even after stripping with Restore Stripping Buffer (Cat. No. 21059)
• Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum- and biotin-free)
• Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes; almost instantaneous for polystyrene microplate wells

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialTBST
FormulationProprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween 20
Recommended StorageUpon receipt store at 4°C.
Concentration1X
For Use With (Application)Western Blot
Physical FormLiquid
Product LineStartingBlock™
Quantity1 L
Unit SizeEach

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.