Pierce™ Protein-Free T20 (PBS) Blocking Buffer, 1 L - FAQs

查看更多产品信息 Pierce™ Protein-Free Blocking Buffer - FAQs (37570, 37571, 37572, 37584, 37573, 37585)

2 个常见问题解答

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the Pierce Protein-Free Blocking Buffer (Cat. No. 37571) animal-origin free (AOF)?

Yes, it is animal-origin free (AOF).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.