SuperBlock™ (TBS) Blocking Buffer, 100 mL - FAQs

将所有存在的胶态碳沉至瓶底，然后将您需要使用的溶液倒出。我们不建议离心或过滤，因为这些操作可能会使产品的封闭能力降低。

卡松防腐剂，一种广谱抗微生物剂，浓度为600 ppm。

在4℃下的有效期为1年。

常规程序是在室温孵育1小时或在4℃孵育过夜。但是，在很多程序中，在室温下孵育3 x 2分钟足以实现有效封闭。

不是，SuperBlock封闭缓冲液不是RNase-free的。

可以， SuperBlock封闭缓冲液能和尼龙膜和硝酸纤维素膜一起工作。

不需要。该产品以1X浓度供应，应“按原液”使用。

不含。除非是名字中含有“T20”的封闭缓冲液，如SuperBlock T20（PBS）封闭缓冲液，含有0.05% Tween 20。

微孔板经过封闭处理后，去除封闭缓冲液并风干数小时。将微孔板与干燥剂一起装在塑料袋中，保存于4℃。为实现最佳的保存，应在24小时后更换干燥剂。微孔板可稳定保存至少12个月。

Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.

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Kathon Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.

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Its shelf life is one year at 4°C.

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Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

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No, SuperBlock Blocking Buffer is not RNase-free.

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Yes, SuperBlock Blocking Buffer works with nylon and nitrocellulose membrane.

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No. It is supplied at 1X concentration and should be used “as is.“

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No. Unless it includes “T20“ in the name, for example, SuperBlock T20 (PBS) Blocking Buffer, which contains 0.05% Tween 20.

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After the plates are blocked, remove the blocking buffer and air dry the plates for several hours. Store plates with desiccant in a plastic bag at 4°C. Change desiccant after 24 hours for optimal storage. The plates should be stable for at least 12 months.

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Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This information is proprietary. The protein concentration in the SuperBlock (TBS) Blocking Buffer has been optimized for direct usage without further dilution, however if desired, the blocking buffer may be diluted with phosphate-buffered saline. For best results, our recommendation is to empirically determine the optimal concentration to use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.