The keratin-binding protein Albatross regulates polarization of epithelial cells.
AuthorsSugimoto M, Inoko A, Shiromizu T, Nakayama M, Zou P, Yonemura S, Hayashi Y, Izawa I, Sasoh M, Uji Y, Kaibuchi K, Kiyono T, Inagaki M,
JournalJ Cell Biol
PubMed ID18838552
'The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane identity. In nonpolarized epithelial cells, Albatross ... More
HSV-1 infection of human corneal epithelial cells: receptor-mediated entry and trends of re-infection.
AuthorsShah A, Farooq AV, Tiwari V, Kim MJ, Shukla D,
JournalMol Vis
PubMed ID21139972
'The human cornea is a primary target for herpes simplex virus-1 (HSV-1) infection. The goals of the study were to determine the cellular modalities of HSV-1 entry into human corneal epithelial (HCE) cells. Specific features of the study included identifying major entry receptors, assessing pH dependency, and determining trends of ... More
A novel function of heparan sulfate in the regulation of cell-cell fusion.
AuthorsO'Donnell CD, Shukla D,
JournalJ Biol Chem
PubMed ID19726670
Despite the important contribution of cell-cell fusion in the development and physiology of eukaryotes, little is known about the mechanisms that regulate this process. Our study shows that glycosaminoglycans and more specifically heparan sulfate (HS) expressed on the cell surface and extracellular matrix may act as negative regulator of cell-cell ... More
HVEM and nectin-1 are the major mediators of herpes simplex virus 1 (HSV-1) entry into human conjunctival epithelium.
AuthorsAkhtar J, Tiwari V, Oh MJ, Kovacs M, Jani A, Kovacs SK, Valyi-Nagy T, Shukla D,
JournalInvest Ophthalmol Vis Sci
PubMed ID18502984
The human conjunctiva is a natural target for herpes simplex virus (HSV)-1 infection. The goals of this study were to investigate the cellular and molecular mechanisms of HSV-1 entry into human conjunctival epithelial (HCjE) cells. Specific features of entry studied included the method of initial viral binding to cells, pH ... More