The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3.
AuthorsHollenbach AD, McPherson CJ, Lagutina I, Grosveld G
JournalBiochim Biophys Acta
PubMed ID11997098
'Pax3 is a member of the paired class homeodomain family of transcription factors and has been demonstrated to be an early marker in myogenic differentiation. To gain a better understanding of how protein-protein interactions regulate Pax3 transcriptional activity, we performed a yeast two-hybrid analysis to identify proteins that interact with ... More
SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.
AuthorsCimadamore F, Amador-Arjona A, Chen C, Huang CT, Terskikh AV,
Journal
PubMed ID23884650
'The transcription factor SRY (sex-determining region)-box 2 (SOX2) is an important functional marker of neural precursor cells (NPCs) and plays a critical role in self-renewal and neuronal differentiation; however, the molecular mechanisms underlying its functions are poorly understood. Using human embryonic stem cell-derived NPCs to model neurogenesis, we found that ... More
The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain.
AuthorsLam PY, Sublett JE, Hollenbach AD, Roussel MF
JournalMol Cell Biol
PubMed ID9858583
The chimeric transcription factor Pax3-FKHR, produced by the t(2;13)(q35;q14) chromosomal translocation in alveolar rhabdomyosarcoma, consists of the two Pax3 DNA binding domains (paired box and homeodomain) fused to the C-terminal forkhead (FKHR) sequences that contain a potent transcriptional activation domain. To determine which of these domains are required for cellular ... More
Pax3-FKHR knock-in mice show developmental aberrations but do not develop tumors.
AuthorsLagutina I, Conway SJ, Sublett J, Grosveld GC
JournalMol Cell Biol
PubMed ID12242297
Alveolar rhabdomyosarcoma is a pediatric disease specified by the recurrent chromosome translocations t(2;13) and t(1;13). These translocations result in the formation of the PAX3-FKHR and PAX7-FKHR fusion genes, which are thought to play a causal role in the genesis of this disease. Although PAX3-FKHR exhibits transforming activity in immortalized fibroblast ... More