Quantifying antibody binding on protein microarrays using microarray nonlinear calibration.
AuthorsYu X, Wallstrom G, Magee DM, Qiu J, Mendoza DE, Wang J, Bian X, Graves M, LaBaer J,
Journal
PubMed ID23662896
'We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacterium tuberculosis models showed that a larger number of putative antigen ... More