TaqMan™ β-Actin Detection Reagents - FAQs

查看更多产品信息 TaqMan™ β-Actin Detection Reagents - FAQs (401846)

3 个常见问题解答

Can I use the TaqMan β-actin Detection Reagents (Cat. No. 401846) as an endogenous control for my gene expression studies?

The TaqMan β-actin Detection Reagents were designed to amplify genomic DNA, and thus are not recommended for use as an endogenous control for gene expression studies. However, we do offer other TaqMan assay reagents that were specifically designed for that application. Two examples are: Human ACTB (Beta Actin) Endogenous Control, Cat. No. 4310881E (TAMRA probe) or Cat. No. 4326315E (MGB probe).

There are many factors to consider when validating a specific endogenous control. For additional information on designing gene expression assays and controls, please refer to the following documents: "Protocol: TaqMan Human Endogenous Control Plate", and User Bulletin #2: "Relative Quantitation of Gene Expression". You can find copies of these documents on our website by entering the titles in the main Search field.

Can TaqMan Beta-actin Detection Reagents, Cat. No. 401846, be used for both human and mouse genomic DNA?

This particular kit was designed for human DNA. Our current Beta-actin Detection Reagents are designed for human targets only. Please check our online search tools for other Beta actin reagents for human (Hs), mouse (Mm) or rat (Rn) targets.

Where can I find the TaqMan Actin Detection Reagents (P/N 401846) manual?

Complete instructions for the TaqMan B-actin Detection Reagents can be found in the manual titled "TaqMan PCR Reagent Kit with Controls" on the product page.