Dynamics of claudins expression in colitis and colitis-associated cancer in rat.
AuthorsArimura Y, Nagaishi K, Hosokawa M,
JournalMethods Mol Biol
PubMed ID21717373
'Claudins comprise a multigene family of 24 species and have been shown to constitute the backbone of tight junction strands in simple epithelial cells and to be directly involved in their barrier functions. Apical-most tight junction protein complexes (TJs) are implicated in inflammatory bowel disease (IBD) pathophysiology. Except for claudin-8, ... More
A microfluidic bioreactor with integrated transepithelial electrical resistance (TEER) measurement electrodes for evaluation of renal epithelial cells.
AuthorsFerrell N, Desai RR, Fleischman AJ, Roy S, Humes HD, Fissell WH,
JournalBiotechnol Bioeng
PubMed ID20552673
We have developed a bilayer microfluidic system with integrated transepithelial electrical resistance (TEER) measurement electrodes to evaluate kidney epithelial cells under physiologically relevant fluid flow conditions. The bioreactor consists of apical and basolateral fluidic chambers connected via a transparent microporous membrane. The top chamber contains microfluidic channels to perfuse the ... More
LGN-dependent orientation of cell divisions in the dermomyotome controls lineage segregation into muscle and dermis.
AuthorsBen-Yair R, Kahane N, Kalcheim C
JournalDevelopment
PubMed ID21852400
The plane of cell divisions is pivotal for differential fate acquisition. Dermomyotome development provides an excellent system with which to investigate the link between these processes. In the central sheet of the early dermomyotome, single epithelial cells divide with a planar orientation. Here, we report that in the avian embryo, ... More
Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.
AuthorsPenes MC, Li X, Nagy JI
JournalEur J Neurosci
PubMed ID16045494
The PDZ domain-containing protein zonula occludens-1 (ZO-1) interacts with several members of the connexin (Cx) family of gap junction-forming proteins and has been localized to gap junctions, including those containing Cx47 in oligodendrocytes. We now provide evidence for ZO-1 expression in astrocytes in vivo and association with astrocytic connexins by ... More