TaqMan™ GAPDH 对照试剂（人类）

Applied Biosystems™ TaqMan™ GAPDH 对照试剂盒提供了在您的实时荧光定量 PCR 反应中将人 GAPDH 用作标准化对照的组分。该试剂盒包含人类对照了解更多信息

货号	数量
402869	100 reactions

货号 402869

价格（CNY)

4,599.00

添加至购物车

100 reactions

价格（CNY)

4,599.00

添加至购物车

Applied Biosystems™ TaqMan™ GAPDH 对照试剂盒提供了在您的实时荧光定量 PCR 反应中将人 GAPDH 用作标准化对照的组分。该试剂盒包含人类对照 RNA、GAPDH TaqMan™ 探针（JOE™ 染料标记探针）和 GAPDH 引物。您可以使用 DNA 或 RNA 作为目标模板。

•预设计的引物和 JOE™ 染料标记的 TaqMan™ 探针无需检测设计

•《快速测定开发指南》可以大大缩短优化时间

•单独的组分可为检测设置提供灵活性

包括综合指南
Applied Biosystems™ 已制定一套综合指南，确保正确使用 Applied Biosystems™ 实时荧光定量 PCR 试剂和仪器。这些指南简单易行。许多传统变量，例如氯化镁浓度和热循环方案本身已经标准化，极大地缩短了测定开发时间。

内置标准化
所有 Applied Biosystems™ 实时荧光定量 PCR 试剂盒均包含了被动内部参比，以对非 PCR 相关荧光波动进行标准化。使用被动内参进行标准化将尽可能地减少各种原因（例如移液误差和样品蒸发）导致的孔间差异。当多个探针（不同的报告基团染料）合并于单管中时，惰性参比对结果的准确性也很关键。

使用说明
TaqMan™ GAPDH 对照试剂可与 TaqMan™ 通用 PCR 预混液或 TaqMan™ PCR 核心试剂联合用于聚合酶链反应 (PCR)。TaqMan™ GAPDH 对照试剂可与 TaqMan™ 一步法 RT-PCR 预混液试剂、TaqMan™ EZ RT-PCR 核心试剂或者 TaqMan™ Gold RT-PCR 试剂联合用于一步法逆转录聚合酶链反应 (RT-PCR)。

PCR 过程以及5'核酸酶过程受专利保护，该等专利归 Roche Molecular Systems, Inc.以及 F. Hoffmann-La Roche, Ltd. 所有

注意：有关有限的标签许可和商标信息，请参见用户手册或包装说明书。仅供科研使用。不适用于诊断用途。

仅供科研使用。不可用于诊断程序。

控制模板人类对照 RNA

产品规格管装

基因符号GADPH

内部探针修改TAMRA™ 淬灭剂 (3')、JOE (5')

反应次数100 次反应

产品线TaqMan

产品类型实时荧光定量 PCR 试剂

数量100 reactions

运输条件干冰

足够用于100 次反应

靶标GAPDH

高 GC PCR 扩增效果低

标签或染料JOE

PCR 方法一步法 RT-qPCR

反应速度标准

Unit SizeEach

内容与储存

对照引物探针套件&模板

常见问题解答 (FAQ)

In the TaqMan GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?

The amplicon length is 226 bases, and is based on the NCBI GenBank accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the “TaqMan Gold RT-PCR Kit” protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

Do your human and rodent GAPDH primers and TaqMan probe sequences detect genomic DNA?

It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:

- The TaqMan GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE dye, spans two exons therefore will not amplify genomic DNA.

- The Human GAPD (GAPDH) Endogenous Control (VIC/ TAMRA Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.

- The probe in the TaqMan Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC dye, does not span an intron and therefore may amplify genomic DNA.