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MicroAmp™ 96-Well Tray/Retainer Set - FAQs

查看更多产品信息 MicroAmp™ 96-Well Tray/Retainer Set - FAQs (403081)

3 个常见问题解答

我的PCR反应管在实验后变形了。问题在哪里？

热循环仪的热盖经过设计可保证对微孔板施加合适的压力，以获得高效的PCR反应。使用PCR反应管时，热盖对反应管的压力过大可导致反应管变形。为避免出现这种情况，我们建议使用下述托盘/固定装置：

•货号4381850，用于Veriti系统的MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），和Veriti热循环仪、SimpliAmp热循环仪和Proflex PCR系统兼容。
•货号403081，MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），可用于7000系统、2720热循环仪和GeneAmp 9700型PCR系统。
•货号4379983，用于VeriFlex模块的MicroAmp 96孔托盘——这一托盘适用于MicroAmp联管（或MicroAmp带盖反应管，0.1 mL），可用于Veriti热循环仪、Veriti快速热循环仪、SimpliAmp热循环仪和Proflex PCR系统。

Why are my PCR tubes crushed/deformed after my PCR run?

The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:

Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.

Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).

Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.

What does the SeqStudio Starter Kit (PN A35000) contain?

The SeqStudio Starter Kit (PN A35000) includes:
- SeqStudio Integrated Capillary Protector (Cat. No. A31923)
- SeqStudio Cathode Buffer Container (Cat. No. A33401)
- Sequencing Standard, BigDye Terminator v3.1, for 3500/SeqStudio Flex (Cat. No. 4404312)
- DS-33 GeneScan Installation Standards with GeneScan 600 LIZ Size Standard v2.0 (Cat. No. 4376911)
- Hi-Di Formamide (Cat. No. 4401457)
- MicroAmp Optical 96-Well Reaction Plate with Barcode (Cat. No. 4306737)
- MicroAmp 96-Well Tray/Retainer Set (Cat. No. 403081)
- MicroAmp Optical 8-Tube Strip, 0.2 mL (Cat. No. 4316567)
- Nuclease-Free Water (not DEPC-Treated) (Cat. No. AM9930)
- Cathode Buffer Container Reservoir Septa for SeqStudio Genetic Analyzer (Cat. No. A35640)
- 96-well Septa for SeqStudio Genetic Analyzer (Cat. No. A35641)
- 8-strip Septa for SeqStudio Genetic Analyzer (Cat. No. A35643)
- SeqStudio Cartridge v2 (PN A41328) (if this component is ordered online the catalog number is A41331)

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.