Cellular characterization of human pluripotent stem cells.
AuthorsQuintanilla RH,
JournalMethods Mol Biol
PubMed ID23546756
Human pluripotent stem cells (PSCs), in particular induced PSCs, are very difficult to derive, grow, and bank. They require extensive amounts of resources and time to render them useful for basic and applied research. As the derivation methods, culture systems and tissues of origin differ, so does the quality of ... More
Applications of quantitative polymerase chain reaction protein assays during reprogramming.
AuthorsRuff D, MacArthur C, Tran H, Bergseid J, Tian J, Shannon M, Chen SM, Fontes A, Laurent L, Swartzman E, Taliana A, Rao M, Lieu PT,
JournalStem Cells Dev
PubMed ID21476854
The capability to reprogram human somatic cells to induced pluripotent stem cells (iPSCs) has opened a new area of biology and provides unprecedented access to patient-specific iPSCs for drug screening, disease models, and transplantation therapies. Although the process of obtaining iPSC lines is technically simple, reprogramming is a slow and ... More