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Gibco™
Ham's F-10 Nutrient Mix, GlutaMAX™ Supplement
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since beenRead more
| Catalog Number | Quantity |
|---|---|
| 41550088 also known as 41550-088 | 10 x 500 mL |
Catalog number 41550088
also known as 41550-088
Price (CNY)
-
Quantity:
10 x 500 mL
Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
The complete formulation is available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
This F-10 is modified as follows:
| With | Without |
| • GlutaMAX™ | • HEPES |
| • Phenol Red |
The complete formulation is available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Osmolality275 - 315 mOsm/kg
Product LineGibco™, GlutaMAX™
Product TypeHam's F-10 Nutrient Mix
Quantity10 x 500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
With AdditivesGlutaMAX, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Without AdditivesNo HEPES
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.