Invitrogen

K-Ras Monoclonal Antibody (9.13)

Name: K-Ras Monoclonal Antibody (9.13)
Brand: Invitrogen
SKU: 415700
Availability: InStock

高级验证

6个已发表图片

7 篇参考文献

查看其他24个K-Ras抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

K-Ras Antibody in Immunocytochemistry (ICC/IF)

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K-Ras Antibody (415700) in ICC/IF

Immunofluorescence analysis of K-Ras was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with K-Ras (9.13) Mouse Monoclonal Antibody (Product # 415700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More

K-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

K-Ras Antibody — Figure 2 miR-200c inhibits K-ras protein expression without affecting KRAS mRNA levels A) Luciferase reporter assay with different breast cancer cell lines. The renilla luciferase reporter containing the 3'UTR of KRAS including the predicted target site of miR-200c (RLuc) or the firefy luciferase control plasmid pGL3 (FLuc) were transfected into the indicated cell lines. Renilla reporter luciferase activity was normalized to the activity of the firefy control as ratio (RLuc/FLuc). Values are stated as mean +- SEM (n=5). B) Luciferase reporter assay upon miR-200c modulation. MDA-MB-436 cells were transfected with either pre-miR-200c (pre) or scrambled pre-miR-control (ctr). BT-474 cells were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr). Relative luciferase activities (RLuc/FLuc) are depicted in the graph. Values are stated as mean +- SEM (n=5). For statistical analysis a student's t-test was performed (**p<0.01; ***p<0.001). C) miR-200c inhibition in the miR-200c high cell lines BT-474 and MCF-7. Indicated cell lines were transfected with either miR-200c inhibitor (inh) or scrambled control inhibitor (ctr) and at 72h post transfection subjected to Western blot analysis (upper panel) or quantitative RT-PCR (lower panel). Values are stated as mean +- SD (n=3). D) miR-200c overexpression in the miR-200c low cell lines MDA-MB-231 and MDA-MB-436. Indicated cell lines were transfected with either pre-miR-200c (pre) or scrambled pre-miR-contro {KD}

Immunogen image

Immunogen Image K-Ras Monoclonal Antibody (9.13)

产品信息

415700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1-2 µg/mL

免疫沉淀 (IP)

5 µg

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

9.13

抗原

Immunogen corresponds to aa 170-180 from P01116-1 (isoform 2A) of human K-Ras.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2532192

产品详细信息

This antibody is specific for human K-Ras (K-Ras2, Ki-Ras, c-K-ras, GTPase KRas) protein (accession # NP_004976.2, P01116), which is 100% homologous with mouse, 95% with rat, and 94% with bovine, respectively. Reactivity has been confirmed on western blots with human HeLa and WI-38 cell lysates as well as rat KNRK and mouse NIH 3T3 cell lysates, and identifies the target band at~21 kDa. The reactivity has been also confirmed with rat KNRK cells using immunoprecipitation and with HeLa cells by immunofluorescence. Based on amino acid sequence homology, reactivity with bovine is also expected.

靶标信息

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: c-K-ras; c-K-ras2 protein; c-Ki-ras; c-Kirsten-ras proto-oncogene; cellular c-Ki-ras2; GTPase KRas; K RAS; K-Ras 2; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homologue 2 (active); p21 protein; RASK; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

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基因别名: AI929937; c-Ki-ras; K-ras; Ki-ras; KRAS; Kras-2; KRAS2; p21; p21B; ras; RASK2

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UniProt ID: (Rat) P08644, (Mouse) P32883

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Entrez Gene ID: (Rat) 24525, (Mouse) 16653

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功能

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding small GTPase

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