Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals.
AuthorsKinzy SA, Caban K, Copeland PR,
JournalNucleic Acids Res
PubMed ID16155186
'Selenocysteine is incorporated into at least 25 human proteins by a complex mechanism that is a unique modification of canonical translation elongation. Selenocysteine incorporation requires the concerted action of a kink-turn structural RNA (SECIS) element in the 3'' untranslated region of each selenoprotein mRNA, a selenocysteine-specific translation elongation factor (eEFSec) ... More
Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus.
AuthorsLiu Y, Schiff M, Serino G, Deng XW, Dinesh-Kumar SP,
JournalPlant Cell
PubMed ID12119369
'The tobacco N gene confers resistance to Tobacco mosaic virus (TMV) and encodes a toll-interleukin-1 receptor/nucleotide binding/Leu-rich repeat class protein. Recent evidence indicates that the Nicotiana benthamiana Rar1 gene (NbRar1), which encodes a protein with a zinc finger motif called CHORD (Cys- and His-rich domain), is required for the function ... More
Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.
'Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an ... More
Partial purification and identification of GDP-mannose 3
AuthorsWolucka BA, Persiau G, Van Doorsselaere J, Davey MW, Demol H, Vandekerckhove J, Van Montagu M, Zabeau M, Boerjan W,
JournalProc Natl Acad Sci U S A
PubMed ID11752432
The first step in the biosynthetic pathway of vitamin C in plants is the formation, at the level of sugar nucleotide, of l-galactosyl residues, catalyzed by a largely unknown GDP-d-mannose 3 ... More
Expression capable library for studies of Neisseria gonorrhoeae, version 1.0.
AuthorsBrettin T, Altherr MR, Du Y, Mason RM, Friedrich A, Potter L, Langford C, Keller TJ, Jens J, Howie H, Weyand NJ, Clary S, Prichard K, Wachocki S, Sodergren E, Dillard JP, Weinstock G, So M, Arvidson CG,
JournalBMC Microbiol
PubMed ID16137322
BACKGROUND: The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge. The recent completion of the genome sequence of the causative agent, Neisseria gonorrhoeae, opens up an entirely new set of approaches for studying ... More