The gateway pDEST17 expression vector encodes a -1 ribosomal frameshifting sequence.
AuthorsBelfield EJ, Hughes RK, Tsesmetzis N, Naldrett MJ, Casey R,
JournalNucleic Acids Res
PubMed ID17272299
'The attB1 site in the Gateway (Invitrogen) bacterial expression vector pDEST17, necessary for in vitro site-specific recombination, contains the sequence AAA-AAA. The sequence A-AAA-AAG within the Escherichia coli dnaX gene is recognized as ''slippery'' and promotes -1 translational frameshifting. We show here, by expressing in E. coli several plant cDNAs ... More
Partial purification and identification of GDP-mannose 3
AuthorsWolucka BA, Persiau G, Van Doorsselaere J, Davey MW, Demol H, Vandekerckhove J, Van Montagu M, Zabeau M, Boerjan W,
JournalProc Natl Acad Sci U S A
PubMed ID11752432
The first step in the biosynthetic pathway of vitamin C in plants is the formation, at the level of sugar nucleotide, of l-galactosyl residues, catalyzed by a largely unknown GDP-d-mannose 3 ... More