BigDye™ Terminator v1.0 & v3.0 5X Sequencing Buffer - FAQs

Are the BigDye Terminator 5X buffer v1.1 & v3.1 and BigDye Terminator 5X buffer v1.0 & v3.0 interchangeable?

The composition of the BigDye Terminator 5X buffer v1.1 & v3.1 and BigDye Terminator 5X buffer v1.0 & v3.0 chemistries is different and therefore should only be used with the matching chemistry. Use of the incorrect BigDye Terminator 5X buffer can negatively impact sequencing results.

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