TaqMan™ MGB 探针
TaqMan™ MGB 探针
Applied Biosystems™

TaqMan™ MGB 探针

Green features
Applied Biosystems TaqMan MGB（小沟结合物）探针为采用 TaqMan 化学用于实时 PCR 应用的双标记探针了解更多信息
Have Questions?
更改视图buttonViewtableView
货号5' 引物修改数量
4316034FAM、NED、TET、VIC6,000 pmol 规格
A40005000ABY6,000 pmol
A40005001ABY20,000 pmol
A40005002ABY50,000 pmol
A40005003JUN6,000 pmol
A40005004JUN20,000 pmol
A40005005JUN50,000 pmol
A40005006Cyanine 56,000 pmol
A40005007Cyanine 520,000 pmol
A40005008Cyanine 550,000 pmol
4316032FAM、NED、TET、VIC50,000 pmol 规格
4316033FAM、NED、TET、VIC20,000 pmol 规格
货号 4316034
价格（CNY)
4,215.00
Each
立刻订购
5' 引物修改:
FAM、NED、TET、VIC
数量:
6,000 pmol 规格
Applied Biosystems TaqMan MGB（小沟结合物）探针为采用 TaqMan 化学用于实时 PCR 应用的双标记探针。TaqMan MGB 探针包含一个 5' 荧光报告基团染料和一个 3' 非荧光淬灭剂 (NFQ)。NFQ 具有背景信号较低的优势，从而能在定量中获得更好的精度。定制 TaqMan MGB 探针经 HPLC 纯化，并提供 FAM、VIC、TET 和 NED 报告基团染料。

更短、更具特异性的探针
TaqMan MGB 探针在3’端包含一个 MGB 部分，可以增加探针的熔解温度 (Tm) 并稳定探针/靶标杂交。这意味着 TaqMan MGB 探针可以比传统探针短很多，提供更好的序列鉴别能力和灵活性，可适应更多靶标。

非荧光淬灭剂 (NFQ) 能够较大程度提高灵敏度
TaqMan MGB 探针包括 NFQ，能够吸取（淬灭）探针另一端的荧光染料标记产生的信号。NFQ 的属性与 MGB 探针的短长度相结合导致背景信号低于非 MGB NFQ 探针。背景更低意味着提高数据的灵敏度和精确度。

产量有保证
您选择的单位规格取决于我们将提供的最小量，而不是合成的起始量。

大致发运时间
北美 4–6 天，欧洲 5-10 天

我们还提供：
•Applied Biosystems 定制引物和其他类型的 TaqMan 探针。了解更多 ›
• TaqMan MGB 探针和引物作为预设计和预配制的 TaqMan assay提供。了解更多 ›
• 商业生产产品和服务，包括 OEM 和 GMP 寡核苷酸生产。了解更多 ›

需要不同染料或浓度或更大批次吗？TaqMan 定制assay和寡核苷酸服务提供定制产品，可满足您的实验需求。了解更多 ›

仅供科研使用。不可用于诊断程序。
规格
3' 引物修改MGB-NFQ
5' 引物修改FAM、NED、TET、VIC
适用于（设备）7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
产品规格管装
环保功能绿色可持续包装
内部探针修改无
产品线TaqMan™
产品类型TaqMan 探针
纯化方法HPLC
纯度或质量等级HPLC
数量6,000 pmol 规格
运输条件室温
适用于（应用）实时荧光定量 PCR (qPCR)
形式液体
标签或染料FAM, VIC, NED, TET
Unit SizeEach
内容与储存
1管，内含溶液形式的双标记探针。

在-15°C 至-25°C 条件下储存

常见问题解答 (FAQ)

I would like to calibrate my Real-Time PCR instrument with a custom dye, which buffer should I use?

Please ask your dye manufacturer for recommendations. If there is no specific recommendation available, try TE Buffer (pH 8.0) to start.

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I determine whether the Tm of my custom probe is appropriate?

In general, the target Tm of a TaqMan probe is 70 degrees C. Using the Primer Express Software, you can check the Tm of a MGB or TAMRA probe probe.

Within the software, go to Primer Probe Test Tool and then copy/paste in your sequence of interest to see the Tm. If you need to check for a MGB based probe, make sure to choose Document Type: TaqMan MGB Quantification. If you need to check for a QSY or TAMRA based probe, make sure to choose Document Type: TaqMan Quantification. Real-Time PCR and Digital PCR Applications

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

I have purchased custom oligos (primers and probes) and I want to make a 20x working stock for a TaqMan assay, similar to the pre-designed assays. How do I dilute and mix my oligos to make a 20x working stock?

Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.

In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.

I am looking to order a molecular probe for validation, using specific sequences. How do I order a custom probe?

We do not offer any custom probes for diagnostic purposes.

We do offer custom TaqMan probes for qPCR experiments, which are for Research Use Only.
TaqMan MGB Probe
TaqMan QSY Probe
TaqMan TAMRA Probe
These above probes can be designed in our online tool, Custom TaqMan Probes.

If you would like to order a pre-mix of primers and probe, please check our custom TaqMan Assays.
You can find more information one the Custom TaqMan Gene Expression Assay page on thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

View all TaqMan™ MGB 探针 FAQs