TaqMan™ TNOS Maize Quantification Kit

The TaqMan™ TNOS Maize Quantification Kit enables the simple and reliable real-time PCR quantification of TNOS DNA in plant, food, and feed samples.

The quantity of transgenic material present in corn can be determined by quantifying the number of copies of TNOS with respect to corn. The Thermo Scientific ™ TaqMan™ TNOS Maize Quantification Kit enables relative quantification of as little as 0.1% of TNOS sequence with respect to total maize in a sample.

The absolute limit of quantification is 20 copies of DNA, and the detection limit of the PCR technique is 5 copies each of TNOS and maize DNA. If the sample contains maize genetic modification (GM) events with several copies of TNOS or another transgenic vegetable species containing TNOS, the amount of transgenic material can be overestimated.

The TaqMan™ TNOS Maize Quantification Kit includes:

• Primers and TaqMan™ probes for real-time PCR detection of the TNOS event and the endogenous maize gene, MSS
• TNOS Standard, a plasmid DNA quantitation standard containing both TNOS and MSS targets
• Enzyme and other buffer components necessary for real-time PCR

The assay is compatible with Applied Biosystems™ QuantStudio 5™ and 7500 Fast real-time PCR systems.

Sample Preparation

DNA sample preparation is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using up to 20 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Part of a Suite of Solutions For GMO Detection and Quantification

The TaqMan™ TNOS Maize Quantification Kit is just one of our many available kits for the sensitive detection of GMO meat species in food and feed samples.