Nunc™ 384-Well, Cell Culture-Treated, Flat/Optical-Bottom Microplate - FAQs

查看更多产品信息 Nunc™ 384-Well, Cell Culture-Treated, Flat/Optical-Bottom Microplate - FAQs (4329MTX)

5 个常见问题解答

Can I use Nunc White 384-Well Microplates for fluorescent assays?

Nunc White 384-Well Microplates can be used for fluorescent applications, but background fluorescence will be much higher compared to Nunc Black 384-Well Microplates.
We recommend the white microplates for scintillation and luminescent detection systems, in which the reflective properties of the microplate enhance the signal without producing any endogenous background signal.

Do you offer Nunc Microplate Lids for the Nunc Black or White 384-Well Microplates?

We offer Nunc Microplate Lids: Cat. No. 448143, that fit both the 384-well microplates and the 1,536-well microplates.

How does cell culturing on Nunc 384-Well Microplates that have a cell culture-treated surface compare with 96-Well Microplates that have a cell culture-treated surface?

The cell culture-treated surfaces are very similar but the wells of the Nunc 384-Well Microplates are smaller than the wells of the Nunc 96-Well Microplate.
Culture recommendations for the Nunc 384-Well Microplates are:
- 15 - 50 µL media per well
- 0.5 - 2.0 x 10^4 cells per well

What are some applications of Nunc 384-Well Black or White Microplates that have a cell culture-treated surface?

We recommend using the Nunc 384-Well Black Microplates for cell-based assays using fluorescent detection. Viability stains such as fluorogenic esterases can be used for proliferation, toxicity, and drug resistance or sensitivity assays. Many types of fluorochrome-conjugated antibodies are available for performing cell-based immunoassays. Fluorescent reporter genes are widely used for determining cell transfection.
We recommend using the Nunc 384-Well White Microplates for cell-based assays using luminescent detection. Viability stains such as luciferin/luciferase can be used for proliferation, toxicity, and drug resistance or sensitivity assays. Cell-based immunoassays can be performed using a HRP-conjugated antibody with a luminol-based substrate. Luciferase reporter genes are widely used for determining cell transfection.

Why do you offer Nunc 384-Well Black or White Microplates when the cells cannot be observed using an inverted light microscope?

We offer Nunc Black or White 384-Well microplates that are intended for use in conjunction with 384-well microplate luminometers and fluorometers that have excitation and detection systems located above the plate. Due to the opaque quality of these microplates, all detection systems must rely on either luminescent or fluorescent reagents, such as viability stains that report cell activity and membrane integrity.