Custom TaqMan™ SNP 基因分型分析,非人类
Custom TaqMan™ SNP 基因分型分析,非人类
Applied Biosystems™

Custom TaqMan™ SNP 基因分型分析,非人类

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Applied Biosystems TaqMan SNP 基因分型Assay使用 TaqMan 5´核酸酶化学试剂扩增和检测纯化基因组 DNA 样品中的特定多态性。每项Assay可对个体进行单核苷酸多态性了解更多信息
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货号数量
4332075M (1000 reactions), made to order
4332076L (2400 reactions), made to order
4332077S (300 reactions), made to order
货号 4332075
价格(CNY)
10,437.00
Each
立刻订购
数量:
M (1000 reactions), made to order
Applied Biosystems TaqMan SNP 基因分型Assay使用 TaqMan 5´核酸酶化学试剂扩增和检测纯化基因组 DNA 样品中的特定多态性。每项Assay可对个体进行单核苷酸多态性 (SNP) 基因分型,包括两个序列特异性引物和两个 TaqMan 小沟结合物 (MGB) 探针以及非荧光淬灭剂 (NFQ)。一个探针标记 VIC 染料以检测等位基因 1 序列;第二个探针标记 FAM 染料以检测等位基因 2 序列。

通过向我们安全可靠的定制Assay设计工具保密提交靶标序列,您可以轻松设计 Custom TaqMan SNP 基因分型Assay,无额外收费。该工具可以生成针对任何生物体中任何 SNP 的Assay设计,提供较大的灵活性以满足您的研究需求。

优势:
经证明—金标准 TaqMan 化学和稳健的Assay设计提供了准确、可重现和可靠的结果
简单—便利的单管形式和简单的工作流程提供了获得可靠结果简易途径;无需优化
灵活—使用我们的安全定制Assay设计工具获得针对任何生物体中任何 SNP 的Assay设计,无需额外收费
检测—对于合成准确度和配方完整性,所有定制Assay均为质量控制检测

大致运输时间
北美 4–6 天,欧洲 6–10 天

自由定制Assay设计工具可生成用于检测 SNP、MNP 以及多达六个碱基的插入缺失的检测。对这些定制Assay进行设计、合成、配制、优化和质量控制检测。

对于 PCR,TaqMan SNP 基因分型Assay仅需要三种反应组分:纯化基因组 DNA (1–20 ng)、分析溶液和 TaqMan 基因分型预混液(或另一种兼容预混液)(单独销售)。

所有Assay设计均是我们行业领先的生物信息学管线的产物,通过利用生产和分析性能数据在十多年的时间内进行了优化。TaqMan Assay已被超过 40,000 篇出版物引用,并且获得超过 350 项专利的支持。

推荐的预混液(单独销售):TaqMan 基因分型预混液

仅供科研使用。不可用于诊断程序。
规格
最大浓度40X
适用于(设备)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
环保功能绿色可持续包装
反应次数5000(384 孔板);1000(96 孔板)
产品线TaqMan™
产品类型Custom TaqMan 基因分型
数量M (1000 reactions), made to order
运输条件室温
样品类型非人
Unit SizeEach
内容与储存
1含 40X(S 和 M 规格)或 80X(L 规格)预配制测定试剂(2 探针和 2 引物)混合物的试管。

在 -15 至 -25°C 下储存。

常见问题解答 (FAQ)

How do I set up a reference panel in the TaqMan Genotyper Software?

A reference panel is helpful in large studies to mark your reference samples. Please follow the directions here on how to set up a reference panel.

How do I enter the polymorphism sequence information (i.e., A, C, G, T) for my assays, and where is this info displayed in the TaqMan Genotyper Software?

The polymorphism sequence info can be entered into the software through Setup >Assays. You can import an assay information file (AIF) that contains this info for your assays (AIFs are shipped with assay orders), or manually enter this info for each assay using the edit assay feature. The polymorphism sequence info will be displayed in the assays table under allele1 base and allele2 base, in the results table in the calls column, in the cluster plot display in the x-axis and y-axis titles, and in the export files as genotypes. If no sequence information is entered for an assay, the default display for genotype calls will use the dye names, such as VIC/VIC, VIC/FAM or FAM/FAM dyes.

What is the bookmarking feature in the TaqMan Genotyper Software, and how would I use it?

Bookmarking is a unique feature in TaqMan Genotyper Software that allows you to tag a data point or well while reviewing results in a Study. For example, in reviewing a cluster plot for an assay, a data point is observed to be somewhat between clusters. You can set a bookmark for this data point to denote this well for further investigation. The bookmark persists between the Results workspace and Quality Control workspace, so you can easily identify the data point in a cluster plot, experiment plate view, or on the samples tab. Bookmarks are cleared upon exit from a Study or exit from the application.

I am getting the message: "An error has occurred. See the log file C:\ProgramFiles\Applied Biosystems\TaqMan Genotyper\config\eclipse\1363113099385.log." How can I fix this?

1.Go to the Start button, then Programs, then TaqMan Genotyper Software
2.Right-click on the program and choose “Run as Administrator”
3.If that does not work, go back to the same menu and choose “Properties”
4.Choose the “Compatibility” tab, and check “Run this program as administrator”
5.Click “Apply”
6.You may+C69 have to restart the computer for the settings to apply

Can I delete an assay or sample from my qPCR study?

An assay or sample may be deleted from a study only if there is no data or wells associated with it. Upon import of an experiment, the software collects all the assays and samples from the plate and lists them in the Setup > Assays or Setup > Samples workspaces. The assays and samples are stored in these workspaces as a library, and remain there even if you delete the experiment from the Study. Deleting the experiment will remove any data (wells) associated with the assays or samples, but not the assays or samples from the library. The assays and samples must then be deleted from these workspaces to remove them from the Study.