BigDye™ Terminator Plus with M13 HiRes Primers

The BigDye™ Terminator BDT Plus Cycle Sequencing Kit with HI-Res Primers is a Sanger sequencing solution designed to enable faster results, improved performance, and enhanced read quality across a broad range of sample types. Combining a newly developed high-processivity enzyme with optimized HI-Res primers, this integrated solution enables improved sequencing accuracy—including enhanced 5' (five-prime) end resolution—while maintaining full compatibility with standard BigDye™ workflows using POP-7™ polymer. Compared to legacy BigDye™ Terminator v3.1 chemistry, the kit helps provide greater speed, flexibility, and data quality without requiring workflow changes.

Key Features and Benefits

• Accelerate sequencing workflows with up to ~50% faster time to results
• Increase success rates and reduce re-runs for greater laboratory efficiency
• Improve performance on challenging templates using a high-processivity enzyme and optimized primers
• Enhance 5' end resolution within standard workflows using POP-7™ polymer
• Reduces the need for dedicated workflows or alternative polymers such as POP-6™
• Maintain existing workflows with full compatibility across BigDye™ protocols and CE instruments

Product Details

The BDT Plus kit with HI-Res primers integrates advanced enzyme chemistry with optimized primer design to improve sequencing performance from template to readout. The high-processivity enzyme enhances incorporation efficiency of dye-labeled terminators, enabling stronger signal intensity and more consistent sequencing results across a wide range of sample types, including GC-rich regions and structurally complex templates.

The inclusion of HI-Res primers further improves sequencing quality by enhancing signal resolution, particularly at the 5' end of reads. This region has historically required modified workflows using alternative polymers such as POP-6™ to achieve improved resolution. In contrast, the combined BDT Plus chemistry and HI-Res primers enable high-quality 5' end data within standard workflows using POP-7™ polymer, simplifying operations and reducing the need for additional method development.

Designed as a ready-to-use solution, the kit functions as a drop-in replacement for existing BigDye™ Terminator chemistries. Laboratories can adopt the kit without changing established protocols, instruments, plastics, or cleanup methods. This compatibility enables users to benefit from improved sequencing performance while maintaining workflow continuity.

The kit supports a wide range of Sanger sequencing research applications, including assay development, mutation detection, plasmid verification, and confirmatory sequencing. It is particularly well suited for laboratories working with diverse or challenging sample types, where consistent performance, high data quality, and workflow efficiency are critical.

By enabling faster turnaround times, improved sequencing performance, and enhanced 5' end resolution, the BDT Plus kit with HI-Res primers helps laboratories increase productivity, reduce re-runs, and generate high-confidence sequencing data.