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7300 Real-Time PCR System with Dell™ Notebook - FAQs

查看更多产品信息 7300 Real-Time PCR System with Dell™ Notebook - FAQs (4351101)

6 个常见问题解答

Why is the ADD DISSOCIATION CURVE button disabled in the Relative Quantification Plate Assay in the SDS software on the ABI PRISM 7000, Applied Biosystems 7300, 7500, 7500 Fast and the 7900HT Systems?

The older versions of the software did not include this feature for all assay types. For the 7500 and 7500fast instruments, the latest software 2.0.5 allows a melt curve to be added to the PCR experiment. The other instruments/software require a separate program for melt curves. To run a Dissociation Curve after running a Relative Quantification Plate (ddCt) Assay, you will need to open a new assay from File -> New, select DISSOCIATION from the assay pull-down menu and run the Dissociation Curve as a separate assay. This will generate a separate SDS file for the Dissociation Run.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the ramp rate for the Applied Biosystems 7300 Real-Time PCR Instrument?

The Standard Mode sample ramp rates are 1.1 °C/sec up and 1.1 °C/sec down.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I export the amplification plot or gene expression plot from the Applied Biosystems 7300, 7500, and 7500 Fast Real-Time PCR System’s software?

With SDS 1.4 software, right-click on the graph, and choose "Export as JPEG" or "Export to Powerpoint". With SDS 2.0.x (for 7500/7500fast only), you can either click on the save icon above the graph "Save as JPEG Image" or click on the Export drop-down arrow (located on the upper toolbar) and choose "Send to Powerpoint"

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I move the Applied Biosystems 7300, 7500, or 7500Fast Real-Time PCR System to another room or another building by myself?

Yes, you can move the instrument carefully on your own. It is recommended that you recalibrate the instrument with the pure dye calibration kit(P/N 4349182, 4349180, 4351151, 4360788, or 4362201) and run an RNase P instrument verification plate (P/N 4351979 or 4350584), after the move.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the extensions of files generated by the ABI PRISM 7000 Sequence Detection System, Applied Biosystems 7300, 7500, 7500Fast, 7900HT Fast Real-Time PCR System, StepOnePlus Real-Time PCR System, and ViiA 7 Real-Time PCR Systems?

All run files have an .SDS or .EDS extension. A Relative Quantification study will have an .SDM or .EDM extension and a template will have an .SDT or .EDT extension. The software for StepOnePlus, ViiA 7, and 7500 2.0.x software have .EDS, .EDT, or .EDM extensions; all other software have .SDS, .SDT, or .SDM extensions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I lost my user manuals for the 7300 and 7500 Fast Real-Time PCR Systems. How can I get an additional copy?

Please contact Technical Support for Real-Time PCR, and provide the serial number of your instrument. We will email you a copy of the user manual upon request.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.