Q: Is it possible to create an indefinite hold (i.e., hold temperature at 4 degrees C forever) on the Applied Biosystems 7300 Real Time PCR System?

It is not possible to program a longer-term infinity hold on the Applied Biosystems 7300 Real Time PCR System. However, you can program a hold for up to 999 minutes and 59 seconds, which should be sufficient in most experiments.

Question 1

When I start the run with 7300, 7500, and 7500 Fast Real-Time PCR Systems, I get an error message that states "Fatal error, block upswitch not engaged". How can I correct this problem?

Accepted Answer

The error message is caused by a shifting of the heated cover. You can correct the problem by following this procedure:

(1) Shut down both the software and the instrument.
(2) Open the front cover by inserting and applying force with a pipette tip or a small Allen wrench on the right side of the front cover.
(3) Inside of the machine, you will see a metal plate with a plastic handle. This metal plate attaches to the heated cover. Pull the handle toward the front of the instrument and then close the front cover.

To prevent this from happening again, make sure you push the PCR plate completely down into the precision plate holder. Also, do not use compression pads on 7300, 7500, and 7500 Fast Real-Time PCR Systems.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Question 2

Is it possible to create an indefinite hold (i.e., hold temperature at 4 degrees C forever) on the Applied Biosystems 7300 Real Time PCR System?

Accepted Answer

It is not possible to program a longer-term &#147;infinity&#148; hold on the Applied Biosystems 7300 Real Time PCR System. However, you can program a hold for up to 999 minutes and 59 seconds, which should be sufficient in most experiments.

Question 3

When using SYBR Green dye chemistry on an Applied Biosystems 7300 Real-Time PCR System, is there an option to set the quencher to reflect the fact that there is no TaqMan probe being used in the reaction?

Accepted Answer

Yes, you can set this as an option. On the Detector Manager of the Applied Biosystems 7300 Real-Time PCR System, simply set the Quencher to "Non Fluorescent".

Question 4

Why do I see an ABI PRISM 7000 Sequence Detection System's run file icon when I transfer an SDS run file from an Applied Biosystems 7900HT Fast Real-Time PCR System or Applied Biosystems 7300 or 7500 Real Time PCR System to my hard drive?

Accepted Answer

All SDS files generated on an ABI PRISM 7000, Applied Biosystems 7300 or 7500 or 7900HT Fast Real Time PCR System have a .sds extension (for example, test101.sds). If your computer has more than one version of SDS software, and you double click on the file icon to open the file, it will be opened from the last SDS software version installed. To work around this, simply launch the appropriate version of SDS software, and open the file from within the software.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

7300 Real-Time PCR System with Dell™ Tower - FAQs

When I start the run with 7300, 7500, and 7500 Fast Real-Time PCR Systems, I get an error message that states "Fatal error, block upswitch not engaged". How can I correct this problem?

Is it possible to create an indefinite hold (i.e., hold temperature at 4 degrees C forever) on the Applied Biosystems 7300 Real Time PCR System?

When using SYBR Green dye chemistry on an Applied Biosystems 7300 Real-Time PCR System, is there an option to set the quencher to reflect the fact that there is no TaqMan probe being used in the reaction?

Why do I see an ABI PRISM 7000 Sequence Detection System's run file icon when I transfer an SDS run file from an Applied Biosystems 7900HT Fast Real-Time PCR System or Applied Biosystems 7300 or 7500 Real Time PCR System to my hard drive?