Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions
Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More
Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
AuthorsSessions, DR; Vick, MM; Fitzgerald, BP
JournalJOURNAL OF ANIMAL SCIENCE
PubMed ID
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.
AuthorsMeyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J
JournalJ Neurosci
PubMed ID16672647
Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.
AuthorsRomieu-Mourez R, Solis M, Nardin A, Goubau D, Baron-Bodo V, Lin R, Massie B, Salcedo M, Hiscott J
JournalCancer Res
PubMed ID17079482
When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we ... More
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
AuthorsItoh S, Kanno S, Gai Z, Suemoto H, Kawakatsu M, Tanishima H, Morimoto Y, Nishioka K, Hatamura I, Yoshida M, Muragaki Y
JournalGenes Cells
PubMed ID18363966
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with ... More
Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
AuthorsGee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
JournalJ Biol Chem
PubMed ID16920714
Human immunodeficiency virus (HIV)-Tat plays an important role in virus replication and in various aspects of host immune responses, including dysregulation of cytokine production. IL-10, an anti-inflammatory cytokine, is up-regulated during the course of HIV infection representing an important pathway by which HIV may induce immunodeficiency. Here we show that ... More
Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.
AuthorsLee JH, Tate CM, You JS, Skalnik DG
JournalJ Biol Chem
PubMed ID17355966
We previously identified a mammalian Set1A complex analogous to the yeast Set1/COMPASS histone H3-Lys4 methyltransferase complex (Lee, J.-H., and Skalnik, D. G. (2005) J. Biol. Chem. 280, 41725-41731). Data base analysis indicates that human Set1A protein shares 39% identity with an uncharacterized SET domain protein, KIAA1076, hereafter denoted ... More
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina
Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 ... More
Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
AuthorsShan, T; Wu, T; Reng, Y; Wang, Y
JournalANIMAL GENETICS
PubMed ID
Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty breed) and Landrace pigs (a leaner breed). In addition, ... More
Temporal changes in bacterial rRNA and rRNA genies in Delaware (USA) coastal waters
The diversity and abundance of bacterial 16S rRNA genes (rDNA) and associated 16S rRNA were assessed to examine community structure and potential activity in a 2 yr seasonal study of marine surface waters along the Delaware (USA) coast. The majority of the ribotypes appearing as rRNA or rDNA were present ... More
Expression profiling and electrophysiological studies suggest a major role for Orai1 in the store-operated Ca(2+) influx pathway of platelets and megakaryocytes
Store-operated Ca(2+) influx represents a major route by which cytosolic Ca(2+) can be elevated during platelet activation, yet its molecular identity in this cell type remains highly controversial. Using quantitative RT-PCR analysis of candidate receptor-operated cation entry pathways in human platelets, we show a 30-fold higher expression of message for ... More
Dried urine spots for detection and quantification of cytomegalovirus in newborns.
AuthorsForman M,Valsamakis A,Arav-Boger R
JournalDiagnostic microbiology and infectious disease
PubMed ID22658885
Dried urine spots (DUS) have been reported to provide a simple screening tool for congenital cytomegalovirus (CMV) infection. We developed a standardized method for CMV quantification from DUS. Two applications of 20 μ L urine remained within the rim of the filter paper disc and were used to determine the ... More
Dissection of the microRNA Network Regulating Hedgehog Signaling in Drosophila.
AuthorsHe T,Fan Y,Wang Y,Liu M,Zhu AJ
JournalFrontiers in cell and developmental biology
PubMed ID35573695
The evolutionarily conserved Hedgehog (Hh) signaling plays a critical role in embryogenesis and adult tissue homeostasis. Aberrant Hh signaling often leads to various forms of developmental anomalies and cancer. Since altered microRNA (miRNA) expression is associated with developmental defects and tumorigenesis, it is not surprising that several miRNAs have been ... More
BMPR2 expression is suppressed by signaling through the estrogen receptor.
BACKGROUND: Studies in multiple organ systems have shown cross-talk between signaling through the bone morphogenetic protein receptor type 2 (BMPR2) and estrogen pathways. In humans, pulmonary arterial hypertension (PAH) has a female predominance, and is associated with decreased BMPR2 expression. The goal of this study was to determine if estrogens ... More
TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response.
AuthorsYang F,Shang L,Wang S,Liu Y,Ren H,Zhu W,Shi X
JournalOxidative medicine and cellular longevity
PubMed ID31214277
Nonalcoholic fatty liver disease (NAFLD) is more sensitive to ischemia and reperfusion injury (IRI), while there are no effective methods to alleviate IRI. Necroptosis, also known as âprogrammed necrosis,â incorporates features of necrosis and apoptosis. However, the role of necroptosis in IRI of the fatty liver remains largely unexplored. In ... More
Analysis of the Catecholaminergic Phenotype in Human SH-SY5Y and BE(2)-M17 Neuroblastoma Cell Lines upon Differentiation.
AuthorsFilograna R,Civiero L,Ferrari V,Codolo G,Greggio E,Bubacco L,Beltramini M,Bisaglia M
JournalPloS one
PubMed ID26317353
Human cell lines are often used to investigate cellular pathways relevant for physiological or pathological processes or to evaluate cell toxicity or protection induced by different compounds, including potential drugs. In this study, we analyzed and compared the differentiating activities of three agents (retinoic acid, staurosporine and 12-O-tetradecanoylphorbol-13-acetate) on the ... More
DNA methylation for cervical cancer screening: a training set in China.
AuthorsKong L,Wang L,Wang Z,Xiao X,You Y,Wu H,Wu M,Liu P,Li L
JournalClinical epigenetics
PubMed ID32576279
BACKGROUND: Despite rapid improvements in DNA methylation tools for cervical cancer screening, few robust, exploratory studies have been performed using the combination of two host genes, EPB41L3 and JAM3, newly developed assays. METHODS: A review of abnormal liquid-based cytology and/or high-risk human papillomavirus (hrHPV) data from outpatient clinics in the ... More
Tissue-specific transcriptome for Dendrobium officinale reveals genes involved in flavonoid biosynthesis.
AuthorsYuan Y,Zhang J,Liu X,Meng M,Wang J,Lin J
JournalGenomics
PubMed ID31678153
Improved RNA preservation for immunolabeling and laser microdissection.
AuthorsBrown AL,Smith DW
JournalRNA (New York, N.Y.)
PubMed ID19850907
Microdissection techniques have the potential to allow for transcriptome analyses in specific populations of cells that are isolated from heterogeneous tissues such as the nervous system and certain cancers. Problematically, RNA is not stable under the labeling conditions usually needed to identify the cells of interest for microdissection. We have ... More
Synthesis and pharmacological evaluation of novel bisindole derivatives bearing oximes moiety: identification of novel proapoptotic agents.
AuthorsQu HE,Huang RZ,Yao GY,Li JL,Ye MY,Wang HS,Liu L
JournalEuropean journal of medicinal chemistry
PubMed ID25841196
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078.
Insights into the interaction between phages and their bacterial hosts are crucial for the development of phage therapy. However, only one study has investigated global gene expression of Clostridioides (formerly Clostridium) difficile carrying prophage, and transcriptional reprogramming during lytic infection has not been studied. Here, we presented the isolation, propagation, ... More
The Role of Fnr Paralogs in Controlling Anaerobic Metabolism in the Diazotroph Paenibacillus polymyxa WLY78.
AuthorsShi H,Li Y,Hao T,Liu X,Zhao X,Chen S
JournalApplied and environmental microbiology
PubMed ID32198173
Fnr is a transcriptional regulator that controls the expression of a variety of genes in response to oxygen limitation in bacteria. Genome sequencing revealed four genes (fnr1, fnr3, fnr5, and fnr7) coding for Fnr proteins in Paenibacillus polymyxa WLY78. Fnr1 and Fnr3 showed more similarity to each other than to ... More
POC1A acts as a promising prognostic biomarker associated with high tumor immune cell infiltration in gastric cancer.
The effect of POC1 centriolar protein A (POC1A) on gastric cancer (GC) has not been clearly defined. In this study, POC1A expression and clinical information in patients with GC were analyzed. Multiple databases were used to investigate the genes that were co-expressed with POC1A and genes whose changes co-occurred with ... More
MdPIN1b encodes a putative auxin efflux carrier and has different expression patterns in BC and M9 apple rootstocks.
AuthorsGan Z,Wang Y,Wu T,Xu X,Zhang X,Han Z
JournalPlant molecular biology
PubMed ID29340953
Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions.
BACKGROUND: A new Bacillus thuringiensis X023 (BtX023) with high insecticidal activity was isolated in Hunan Province, China. The addition of metals (Cu, Fe, Mg and Mn) to the medium could influence the formation of spores and/or insecticidal crystal proteins (ICPs). In previous studies, Cu ions considerably increased the synthesis of ... More
Mutual Interaction of Clinical Factors and Specific microRNAs to Predict Mild Cognitive Impairment in Patients Receiving Hemodialysis.
Cognitive impairment (CI) is not uncommon in dialysis patients. Various factors have been implicated. This study aims to examine mutual interaction of various clinical factors for CI in patients receiving hemodialysis. A total of 48 hemodialysis patients in outpatient clinic were recruited from 2015 to 2017. Demographics, circulating uremic toxin ... More
In Vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum-Sensing Inhibitors.
Clematis florida Thun (CfT) is an ornamental and medicinal plant. It is a cold resistant but heat sensitive species and deserves to be further investigated to improve its adaptability to heat stress. Exploring the molecular mechanism potential via an omic-analysis constitutes a promising approach towards improving heat tolerance of CfT. ... More
Comprehensive Genome-Wide Analysis of Thaumatin-Like Gene Family in Four Cotton Species and Functional Identification of GhTLP19 Involved in Regulating Tolerance to Verticillium dahlia and Drought.
AuthorsLi Z,Wang X,Cui Y,Qiao K,Zhu L,Fan S,Ma Q
JournalFrontiers in plant science
PubMed ID33193513
Thaumatin-like proteins (TLPs) present in the form of large multigene families play important roles in biotic stress and abiotic stress. However, there has been no systematic analysis of the TLPs in cotton. In this study, comprehensive identification and evolutionary analysis of TLPs in four species of cotton were conducted. In ... More
IP6K1 is essential for chromatoid body formation and temporal regulation of Tnp2 and Prm2 expression in mouse spermatids.
AuthorsMalla AB,Bhandari R
JournalJournal of cell science
PubMed ID28743739
A unique invertase is important for sugar absorption of an obligate biotrophic pathogen during infection.
Antiviral activity of Thiosemicarbazones derived from α-amino acids against Dengue virus.
AuthorsPadmanabhan P,Khaleefathullah S,Kaveri K,Palani G,Ramanathan G,Thennarasu S,Tirichurapalli Sivagnanam U
JournalJournal of medical virology
PubMed ID27490721
microRNA-100 functions as a tumor suppressor in non-small cell lung cancer via regulating epithelial-mesenchymal transition and Wnt/β-catenin by targeting HOXA1.
AuthorsHan W,Ren X,Yang Y,Li H,Zhao L,Lin Z
JournalThoracic cancer
PubMed ID32364673
BACKGROUND: Nonâsmall cell lung cancer (NSCLC) is a leading subtype in lung cancer, with high morbidities and mortalities worldwide. microRNA (miRNA) has appeared to play indispensable roles in a variety of solid carcinomas. The current study focused on the functions of miRâ100 in NSCLC. METHODS: qRTâPCR was performed to detect ... More
Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression.
Chemoresistance in multidrug-resistant (MDR) cells over expressing P-glycoprotein (P-gp) encoded by the MDR1 gene, is a major obstacle to successful chemotherapy for colorectal cancer. Previous studies have indicated that sinomenine can enhance the absorption of various P-gp substrates. In the present study, we investigated the effect of sinomenine on the ... More
Controlling gene activation by enhancers through a drug-inducible topological insulator.
While regulation of gene-enhancer interaction is intensively studied, its application remains limited. Here, we reconstituted arrays of CTCF-binding sites and devised a synthetic topological insulator with tetO for chromatin-engineering (STITCH). By coupling STITCH with tetR linked to the KRAB domain to induce heterochromatin and disable the insulation, we developed a ... More
Genome-wide translational profiling of amygdala Crh-expressing neurons reveals role for CREB in fear extinction learning.
Fear and extinction learning are adaptive processes caused by molecular changes in specific neural circuits. Neurons expressing the corticotropin-releasing hormone gene (Crh) in central amygdala (CeA) are implicated in threat regulation, yet little is known of cell type-specific gene pathways mediating adaptive learning. We translationally profiled the transcriptome of CeA ... More
Denitrifying bacterial communities affect current production and nitrous oxide accumulation in a microbial fuel cell.
AuthorsVilar-Sanz A,Puig S,García-Lledó A,Trias R,Balaguer MD,Colprim J,Bañeras L
JournalPloS one
PubMed ID23717427
The biocathodic reduction of nitrate in Microbial Fuel Cells (MFCs) is an alternative to remove nitrogen in low carbon to nitrogen wastewater and relies entirely on microbial activity. In this paper the community composition of denitrifiers in the cathode of a MFC is analysed in relation to added electron acceptors ... More
Sirtuin 3 improves fatty acid metabolism in response to high nonesterified fatty acids in calf hepatocytes by modulating gene expression.
The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic testing. The World Health Organization (WHO) recommends a standard assay that includes an RNA extraction step from a nasopharyngeal (NP) swab followed by reverse transcriptionâquantitative polymerase chain reaction (RT-qPCR) to detect the purified SARS-CoV-2 RNA. The current global ... More
HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway.
The mortality rate of ovarian cancer is the highest among gynaecological cancers, primarily due to metastatic symptoms. Recent studies have shown that HOX genes are crucial in tumour progression, but the underlying mechanisms remain unclear. Here, HOXC10 expression was examined in ovarian cancer tissues. The function of HOXC10 in ovarian ... More
OmpW is positively regulated by iron via Fur, and negatively regulated by SoxS contribution to oxidative stress resistance in Escherichia coli.
AuthorsZhang P,Ye Z,Ye C,Zou H,Gao Z,Pan J
JournalMicrobial pathogenesis
PubMed ID31634530
Detection and Quantification Methods for Viable but Non-culturable (VBNC) Cells in Process Wash Water of Fresh-Cut Produce: Industrial Validation.
AuthorsTruchado P,Gil MI,Larrosa M,Allende A
JournalFrontiers in microbiology
PubMed ID32431672
The significance of viable but non-culturable (VBNC) cells in the food industry is not well known, mainly because of the lack of suitable detection methodologies to distinguish them from dead cells. The study aimed at the selection of the method to differentiate dead and VBNC cells of Listeria monocytogenes in ... More
Ophiopogonin D and EETs ameliorate Ang II-induced inflammatory responses via activating PPARα in HUVECs.
AuthorsHuang X,Wang Y,Zhang Z,Wang Y,Chen X,Wang Y,Gao Y
JournalBiochemical and biophysical research communications
PubMed ID28587983
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers.
AuthorsMaroniche GA,Sagadín M,Mongelli VC,Truol GA,del Vas M
JournalVirology journal
PubMed ID21679431
BACKGROUND: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers might unveil the molecular basis of viral transmission. Quantitative real-time RT-PCR (RT-qPCR) is currently the most accurate and sensitive ... More
Quantitative Analysis of Galectin-3 Expression in Benign and Malignant Thyroid Nodules.
AuthorsŠamija I,Mateša N,Tadinac S,Jukić T
JournalActa clinica Croatica
PubMed ID34219881
In this study, galectin-3 was analyzed as a potential marker for preoperative detection of malignant thyroid lesions. Galectin-3 expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) in preoperative thyroid fine-needle aspirates from 245 patients with thyroid nodules. Out of 245 samples, 238 were adequate for analysis ... More
Molecular cloning and functional characterization of multiple NADPH-cytochrome P450 reductases from Andrographis paniculata.
AuthorsLin H,Wang J,Qi M,Guo J,Rong Q,Tang J,Wu Y,Ma X,Huang L
JournalInternational journal of biological macromolecules
PubMed ID28410952
The transformation from anammox granules to deammonification granules in micro-aerobic system by facilitating indigenous ammonia oxidizing bacteria.
AuthorsWang X,Gao D
JournalBioresource technology
PubMed ID29195156
Methyl jasmonate promote protostane triterpenes accumulation by up-regulating the expression of squalene epoxidases in Alisma orientale.
Protostane triterpenes, which are found in Alisma orientale, are tetracyclic triterpenes with distinctive pharmacological activities. The natural distribution of protostane triterpenes is limited mainly to members of the botanical family Alismataceae. Squalene epoxidase (SE) is the key rate-limiting enzyme in triterpene biosynthesis. In this study, we report the characterization of ... More
LncRNA CANT1 suppresses retinoblastoma progression by repellinghistone methyltransferase in PI3Kγ promoter.
Retinoblastoma (RB) is the most common malignant intraocular tumor of childhood. Recent studies have shown that long noncoding RNAs (lncRNAs), which are longer than 200âbp and without protein-coding ability, are key regulators of tumorigenesis. However, the role of lncRNAs in retinoblastoma remains to be elucidated. In this study, we found ... More
The relationship between estrogen-induced phenotypic transformation and proliferation of vascular smooth muscle and hypertensive intracerebral hemorrhage.
AuthorsXia X,Zhou C,He X,Liu C,Wang G,Sun X
JournalAnnals of translational medicine
PubMed ID32647687
BACKGROUND: To explore the effect of estrogen on human cerebral vascular smooth muscle cells (VSMCs) and to clarify the molecular mechanism of estrogen inhibition of VSMC proliferation, which could provide an important reference basis for the clinical treatment of hypertensive intracerebral hemorrhage. METHOD: Firstly, the effects of different concentrations of ... More
Contribution of senescence in human endometrial stromal cells during proliferative phase to embryo receptivity†.
AuthorsTomari H,Kawamura T,Asanoma K,Egashira K,Kawamura K,Honjo K,Nagata Y,Kato K
JournalBiology of reproduction
PubMed ID32285109
Successful assisted reproductive technology pregnancy depends on the viability of embryos and endometrial receptivity. However, the literature has neglected effects of the endometrial environment during the proliferative phase on implantation success or failure. Human endometrial stromal cells (hESCs) were isolated from endometrial tissues sampled at oocyte retrieval during the proliferative ... More
Full mtDNA genome sequencing of Brazilian admixed populations: A forensic-focused evaluation of a MPS application as an alternative to Sanger sequencing methods.
Association of pro-inflammatory cytokines and iron regulatory protein 2 (IRP2) with Leishmania burden in canine visceral leishmaniasis.
Authorsdo Nascimento PR,Martins DR,Monteiro GR,Queiroz PV,Freire-Neto FP,Queiroz JW,Morais Lima AL,Jeronimo SM
JournalPloS one
PubMed ID24146743
Leishmania infantum infection in humans and dogs can evolve with a wide range of clinical presentations, varying from asymptomatic infections to visceral leishmaniasis. We hypothesized that the immune response elicited by L. infantum infection could modulate whether the host will remain asymptomatic or progress to disease. A total of 44 ... More
Correlation Between Single Nucleotide Polymorphisms of the rs664589 Locus in the Long-Chain Noncoding RNA Lung Adenocarcinoma Metastasis-Associated Gene 1, Hypertension, and Its Mechanism.
AuthorsMou X,Wang J,Wang L,Wang S
JournalGenetic testing and molecular biomarkers
PubMed ID32109146
Long non-coding RNA SNHG20 predicts a poor prognosis for HCC and promotes cell invasion by regulating the epithelial-to-mesenchymal transition.
Human Intestinal Tissue Explant Exposure to Silver Nanoparticles Reveals Sex Dependent Alterations in Inflammatory Responses and Epithelial Cell Permeability.
AuthorsGokulan K,Williams K,Orr S,Khare S
JournalInternational journal of molecular sciences
PubMed ID33374948
Consumer products manufactured with antimicrobial silver nanoparticles (AgNPs) may affect the gastrointestinal (GI) system. The human GI-tract is complex and there are physiological and anatomical differences between human and animal models that limit comparisons between species. Thus, assessment of AgNP toxicity on the human GI-tract may require tools that allow ... More
Glucosamine has an antiallergic effect in mice with allergic asthma and rhinitis.
AuthorsJung AY,Heo MJ,Kim YH
JournalInternational forum of allergy & rhinology
PubMed ID28558148
Reduction of depression-like behavior in rat model induced by ShRNA targeting norepinephrine transporter in locus coeruleus.
Depression may be associated with reduced monoamine neurotransmission, particularly serotonin and norepinephrine (NE). Reuptake of NE by the norepinephrine transporter (NET) is the primary mechanism by which many of the antidepressants are high-affinity substrates for NET. This study aimed to examine the effect of lentivirus-mediated shRNA targeting NET in locus ... More
Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice.
Neurons located in dorsal root ganglia (DRG) are crucial for transmitting peripheral sensations such as proprioception, touch, temperature, and nociception to the spinal cord before propagating these signals to higher brain structures. To date, difficulty in identifying modality-specific DRG neurons has limited our ability to study specific populations in detail. ... More
Simple hypertrophic tonsils have more active innate immune and inflammatory responses than hypertrophic tonsils with recurrent inflammation in children.
AuthorsHuang Q,Hua H,Li W,Chen X,Cheng L
JournalJournal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale
PubMed ID32487224
BACKGROUND: Tonsil hypertrophy has negative impact on childrenâs health, but its pathogenesis remains obscure despite the fact that numerous bacteriological studies have been carried out. Understanding the innate immune and inflammatory states of hypertrophic tonsils with different clinical manifestations is of great significance for defining the pathogenesis of tonsil hypertrophy ... More
T-type calcium channel inhibition restores sensitivity to MAPK inhibitors in de-differentiated and adaptive melanoma cells.
BACKGROUND: Drug resistance remains as one of the major challenges in melanoma therapy. It is well known that tumour cells undergo phenotypic switching during melanoma progression, increasing melanoma plasticity and resistance to mitogen-activated protein kinase inhibitors (MAPKi). METHODS: We investigated the melanoma phenotype switching using a partial reprogramming model to ... More
Role and therapeutic effects of skeletal muscle-derived non-myogenic cells in a rat myocardial infarction model.
AuthorsIseoka H,Miyagawa S,Saito A,Harada A,Sawa Y
JournalStem cell research & therapy
PubMed ID32070429
BACKGROUND: Transplantation of skeletal myoblast sheets is a promising strategy for the treatment of heart failure, and its therapeutic effects have already been proven in both animal disease models and clinical trials. Myoblast sheets reportedly demonstrate their therapeutic effects by producing many paracrine factors. Although the quality of processed cells ... More
Decreased expression of miR-150, miR146a and miR424 in type 1 diabetic patients: Association with ongoing islet autoimmunity.
AuthorsWang G,Gu Y,Xu N,Zhang M,Yang T
JournalBiochemical and biophysical research communications
PubMed ID28733034
Differential Expression and Significance of Circulating microRNAs in Cerebrospinal Fluid of Acute Encephalitis Patients Infected with Japanese Encephalitis Virus.
AuthorsGoswami S,Banerjee A,Kumari B,Bandopadhyay B,Bhattacharya N,Basu N,Vrati S,Banerjee A
JournalMolecular neurobiology
PubMed ID26860411
Impact of varied combinatorial mixture of non-fishmeal ingredients on growth, metabolism, immunity and gut microbiota of Lates calcarifer (Bloch, 1790) fry.
The search for suitable fish meal replacements in aqua-diets is a salient agenda in the constant effort of making aquaculture practices more sustainable. In this study, we tested four customised diets composed by systematic inclusion of pre-selected fish meal substitutes, lupin kernel meal, BSF meal, TH and PBM on growth, ... More
Intra-articular autologous uncultured adipose-derived stromal cell transplantation inhibited the progression of cartilage degeneration.
JournalJournal of orthopaedic research : official publication of the Orthopaedic Research Society
PubMed ID30378173
ADAMTS8 is frequently down-regulated in colorectal cancer and functions as a tumor suppressor.
AuthorsLi L,Yuan S,Zhao X,Luo T
JournalBiochemical and biophysical research communications
PubMed ID32033751
Inhibition of poly(ADP-ribose) polymerase-1 alters expression of mitochondria-related genes in PC12 cells: relevance to mitochondrial homeostasis in neurodegenerative disorders.
JournalBiochimica et biophysica acta. Molecular cell research
PubMed ID29128369
Analysis of deubiquitinase OTUD5 as a biomarker and therapeutic target for cervical cancer by bioinformatic analysis.
AuthorsBai M,Che Y,Lu K,Fu L
JournalPeerJ
PubMed ID32655987
OTU deubiquitinase 5 (OTUD5), as a member of the ovarian tumor protease (OTU) family, was previously reported to play important roles in DNA repair and immunity. However, little is known about its function in tumors. Cervical cancer is a malignant tumor that seriously endangers the lives of women. Here, we ... More
OsPLS4 Is Involved in Cuticular Wax Biosynthesis and Affects Leaf Senescence in Rice.
Leaf senescence is one of the most common factors that affects the growth and yield of rice. Although numerous genes affecting leaf senescence have been identified, few involved in cuticular wax synthesis have been described for rice premature leaf senescence. Here, we cloned and characterized Premature Leaf Senescence 4 (PLS4) ... More
Human conditionally immortalized neural stem cells improve locomotor function after spinal cord injury in the rat.
INTRODUCTION: A growing number of studies have highlighted the potential of stem cell and more-differentiated neural cell transplantation as intriguing therapeutic approaches for neural repair after spinal cord injury (SCI). METHODS: A conditionally immortalized neural stem cell line derived from human fetal spinal cord tissue (SPC-01) was used to treat ... More
Liposome-Protamine-DNA Nanoparticle-Mediated Delivery of Short Hairpin RNA Targeting Brachyury Inhibits Chordoma Cell Growth.
AuthorsHu Y,Singh R,Deng Z,Mintz A,Hsu W
JournalJournal of biomedical nanotechnology
PubMed ID29360338
Recent evidence suggests that brachyury is a crucial molecular driver in the initiation and propagation of chordoma. However, no small molecules have been used to specifically target brachyury. Short hairpin RNA (shRNA) has therapeutic promise for the genetic treatment of cancer, but the usage of shRNA therapeutics is limited by ... More
AuthorsAsano S,Arvapalli R,Manne ND,Maheshwari M,Ma B,Rice KM,Selvaraj V,Blough ER
JournalInternational journal of nanomedicine
PubMed ID26491293
The severe inflammation observed during sepsis is thought to cause diaphragm dysfunction, which is associated with poor patient prognosis. Cerium oxide (CeO(2)) nanoparticles have been posited to exhibit anti-inflammatory and antioxidative activities suggesting that these particles may be of potential use for the treatment of inflammatory disorders. To investigate this ... More
Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet.
AuthorsPeñaranda DS,Bäuerl C,Tomás-Vidal A,Jover-Cerdá M,Estruch G,Pérez Martínez G,Martínez Llorens S
JournalInternational journal of molecular sciences
PubMed ID33066515
The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in ... More
LINC01436 Promotes the Progression of Gastric Cancer via Regulating miR-513a-5p/APE1 Axis.
AuthorsLu MD,Liu D,Li YX
JournalOncoTargets and therapy
PubMed ID33116638
BACKGROUND: Gastric cancer (GC) is one of the deadliest cancer worldwide. Multiple long non-coding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumor suppressors in GC. In this study, we aimed to probe into the effect of LINC01436 on GC progression. METHODS: LINC01436 and miR-513a-5p expressions in GC ... More
LINC00319 acts as a microRNA-335-5p sponge to accelerate tumor growth and metastasis in gastric cancer by upregulating ADCY3.
AuthorsZou J,Wu K,Lin C,Jie ZG
JournalAmerican journal of physiology. Gastrointestinal and liver physiology
Cell-cell interactions are vital for embryonic organ development and normal function of differentiated cells and tissues. In this study we have developed a self-assembled monolayer-based co-culture system to study tooth morphogenesis. Specifically, we designed a 2-D microenvironment present in the dental tissue by creating a well-structured, laterally organized epithelial and ... More
Galectin‑3 induces the phenotype transformation of human vascular smooth muscle cells via the canonical Wnt signaling.
AuthorsTian L,Chen K,Cao J,Han Z,Wang Y,Gao L,Fan Y,Wang C
JournalMolecular medicine reports
PubMed ID28393190
Lithium response in bipolar disorder correlates with improved cell viability of patient derived cell lines.
AuthorsPaul P,Iyer S,Nadella RK,Nayak R,Chellappa AS,Ambardar S,Sud R,Sukumaran SK,Purushottam M,Jain S,ADBS Consortium (ADBS: The Accelerator program for Discovery in Brain disorders using Stem cells),Viswanath B
JournalScientific reports
PubMed ID32366893
Lithium is an effective, well-established treatment for bipolar disorder (BD). However, the mechanisms of its action, and reasons for variations in clinical response, are unclear. We used neural precursor cells (NPCs) and lymphoblastoid cell lines (LCLs), from BD patients characterized for clinical response to lithium (using the âAlda scaleâ and ... More
Identification and genetic analysis of qCL1.2, a novel allele of the "green revolution" gene SD1 from wild rice (Oryza rufipogon) that enhances plant height.
BACKGROUND: The exploitation of novel alleles from wild rice that were lost during rice cultivation could be very important for rice breeding and evolutionary studies. Plant height (PH) was a target of artificial selection during rice domestication and is still a target of modern breeding. The âgreen revolutionâ gene semi-dwarf ... More
Disturbed P53-MDM2 Feedback Loop Contributes to Thoracic Aortic Dissection Formation and May be a Result of TRIM25 Overexpression.
BACKGROUND: The aim of this study was to determine whether progesterone could inhibit the growth of lung adenocarcinoma cells via membrane progesterone receptor alpha (mPRα) and elucidate its potential mechanism. The relationship between mPRα expression and the survival prognosis of lung adenocarcinoma patients was studied. METHODS: A mPRα knockdown lung ... More
Down-regulated microRNA-375 expression as a predictive biomarker in non-small cell lung cancer brain metastasis and its prognostic significance.
Boron improves cardiac contractility and fibrotic remodeling following myocardial infarction injury.
AuthorsBouchareb R,Katz M,Saadallah N,Sassi Y,Ali S,Lebeche D
JournalScientific reports
PubMed ID33051505
Myocardial fibrosis is a major determinant of clinical outcomes in heart failure (HF) patients. It is characterized by the emergence of myofibroblasts and early activation of pro-fibrotic signaling pathways before adverse ventricular remodeling and progression of HF. Boron has been reported in recent years to augment the innate immune system ... More
Neonatal endotoxin stimulation is associated with a long-term bronchiolar epithelial expression of innate immune and anti-allergic markers that attenuates the allergic response.
AuthorsGarcía LN,Leimgruber C,Nicola JP,Quintar AA,Maldonado CA
JournalPloS one
PubMed ID32379832
Allergic asthma is the most common phenotype of the pathology, having an early-onset in childhood and producing a Th2-driven airways remodeling process that leads to symptoms and pathophysiological changes. The avoidance of aeroallergen exposure in early life has been shown to prevent asthma, but without repeated success and with the ... More
Assessing the Use of PCR To Screen for Shedding of Salmonella enterica in Infected Humans.
AuthorsLloyd T,Bender M,Huang S,Brown R,Shiau R,Yette E,Shemsu M,Pandori M
JournalJournal of clinical microbiology
PubMed ID32376667
Recovery from enteric bacterial illness often includes a phase of organismal shedding over a period of days to months. The monitoring of this process through laboratory testing forms the foundation of public health action to prevent further transmission. Regulations in most jurisdictions in the United States exclude individuals who continue ... More
Gene deletion of the Na(+)-glucose cotransporter SGLT1 ameliorates kidney recovery in a murine model of acute kidney injury induced by ischemia-reperfusion.
AuthorsNespoux J,Patel R,Hudkins KL,Huang W,Freeman B,Kim YC,Koepsell H,Alpers CE,Vallon V
JournalAmerican journal of physiology. Renal physiology
PubMed ID30995111
Renal Na(+)-glucose cotransporter SGLT1 mediates glucose reabsorption in the late proximal tubule, a hypoxia-sensitive tubular segment that enters the outer medulla. Gene deletion in mice (Sglt1(â/â)) was used to determine the role of the cotransporter in acute kidney injury induced by ischemia-reperfusion (IR), including the initial injury and subsequent recovery ... More
Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.
The NAD(+)-dependent deacetylase Sirtuin 1 (SIRT1) is down-regulated in triplenegative breast cancer. To determine the mechanistic basis by which reduced SIRT1 expression influences processes related to certain aggressive cancers, we examined the consequences of depleting breast cancer cells of SIRT1. We discovered that reducing SIRT1 levels decreased the expression of ... More
Deregulation of the endometrial stromal cell secretome precedes embryo implantation failure.
Down-regulation of COX-2 activity by 1α,25(OH)(2)D(3) is VDR dependent in endothelial cells transformed by Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor.
AuthorsTapia C,Zamarreño F,Salvador GA,Casali CI,Viso J,Fernandez MDC,White JH,González-Pardo V
JournalHeliyon
PubMed ID33072916
Our previous reports showed that 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)) has antiproliferative actions in endothelial cells stably expressing viral G protein-coupled receptor (vGPCR) associated with the pathogenesis of Kaposi's sarcoma. It has been reported that COX-2 enzyme, involved in the tumorigenesis of many types of cancers, is induced by vGPCR. Therefore, we ... More
Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
JournalInternational journal of molecular sciences
PubMed ID32380646
Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains. Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat. Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has ... More
Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming.
AuthorsGarcía-Martínez T,Vendrell-Flotats M,Martínez-Rodero I,Ordóñez-León EA,Álvarez-Rodríguez M,López-Béjar M,Yeste M,Mogas T
JournalInternational journal of molecular sciences
PubMed ID33066129
This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental ... More
The Impact of Adenosine Fast Induction of Myocardial Arrest during CABG on Myocardial Expression of Apoptosis-Regulating Genes Bax and Bcl-2.
Background. We studied the effect of fast induction of cardiac arrest with denosine on myocardial bax and bcl-2 expression. Methods and Results. 40 elective CABG patients were allocated into two groups. The adenosine group (n = 20) received 250âμg/kg adenosine into the aortic root followed by blood potassium cardioplegia. The ... More
The expression of microRNA-324-3p as a tumor suppressor in nasopharyngeal carcinoma and its clinical significance.
AuthorsZhang HQ,Sun Y,Li JQ,Huang LM,Tan SS,Yang FY,Li H
JournalOncoTargets and therapy
PubMed ID29066913
OBJECTIVE: This study aimed to determine the expression, clinical significance, and possible biologic function of microRNA-324-3p in nasopharyngeal carcinoma (NPC) tissues. METHODS: In total, 54 NPC and 35 control tissues were collected. The correlation between miR-324-3p expression and the clinicopathologic characteristics was analyzed. A dual-luciferase reporter gene assay was employed ... More
PAK5 facilitates the proliferation, invasion and migration in colorectal cancer cells.
Colorectal cancer (CRC) is the thirdâmost common cancer around the world, accounting for approximately 10% of cancerârelated mortality. Deeper molecular understanding of colorectal carcinogenesis will provide evidences for identification of early diagnostic indicators and novel therapeutic strategies for CRC treatment. The p21(cdc42/rac1)âactivated kinase 5 (PAK5) has been reported to be ... More
Reduced endogenous secretory RAGE in blood and bronchoalveolar lavage fluid is associated with poor prognosis in idiopathic pulmonary fibrosis.
BACKGROUND: The endogenous secretory receptor for advanced glycation end products (esRAGE) is a soluble isoform produced by alternative splicing of the RAGE gene. The isoform has anti-inflammatory properties due to its inhibition of the RAGE/ligand interaction and is reduced in the lung tissue of patients with idiopathic pulmonary fibrosis (IPF). ... More
Association of regulatory TPH2 polymorphisms with higher reduction in depressive symptoms in children and adolescents treated with fluoxetine.
Ergothioneine (ET) is a naturally occurring antioxidant that is synthesized by non-yeast fungi and certain bacteria. ET is not synthesized by animals, including humans, but is avidly taken up from the diet, especially from mushrooms. In the current study, we elucidated the effect of ET on the hCMEC/D3 human brain ... More
Transcriptomic Analysis of Host Immune Response in the Skin of Chickens Infected with Marek's Disease Virus.