Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions
Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More
Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
AuthorsSessions, DR; Vick, MM; Fitzgerald, BP
JournalJOURNAL OF ANIMAL SCIENCE
PubMed ID
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.
AuthorsMeyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J
JournalJ Neurosci
PubMed ID16672647
Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.
AuthorsRomieu-Mourez R, Solis M, Nardin A, Goubau D, Baron-Bodo V, Lin R, Massie B, Salcedo M, Hiscott J
JournalCancer Res
PubMed ID17079482
When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we ... More
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
AuthorsItoh S, Kanno S, Gai Z, Suemoto H, Kawakatsu M, Tanishima H, Morimoto Y, Nishioka K, Hatamura I, Yoshida M, Muragaki Y
JournalGenes Cells
PubMed ID18363966
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with ... More
Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
AuthorsGee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
JournalJ Biol Chem
PubMed ID16920714
Human immunodeficiency virus (HIV)-Tat plays an important role in virus replication and in various aspects of host immune responses, including dysregulation of cytokine production. IL-10, an anti-inflammatory cytokine, is up-regulated during the course of HIV infection representing an important pathway by which HIV may induce immunodeficiency. Here we show that ... More
Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.
AuthorsLee JH, Tate CM, You JS, Skalnik DG
JournalJ Biol Chem
PubMed ID17355966
We previously identified a mammalian Set1A complex analogous to the yeast Set1/COMPASS histone H3-Lys4 methyltransferase complex (Lee, J.-H., and Skalnik, D. G. (2005) J. Biol. Chem. 280, 41725-41731). Data base analysis indicates that human Set1A protein shares 39% identity with an uncharacterized SET domain protein, KIAA1076, hereafter denoted ... More
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina
Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 ... More
Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
AuthorsShan, T; Wu, T; Reng, Y; Wang, Y
JournalANIMAL GENETICS
PubMed ID
Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty breed) and Landrace pigs (a leaner breed). In addition, ... More
Temporal changes in bacterial rRNA and rRNA genies in Delaware (USA) coastal waters
The diversity and abundance of bacterial 16S rRNA genes (rDNA) and associated 16S rRNA were assessed to examine community structure and potential activity in a 2 yr seasonal study of marine surface waters along the Delaware (USA) coast. The majority of the ribotypes appearing as rRNA or rDNA were present ... More
Expression profiling and electrophysiological studies suggest a major role for Orai1 in the store-operated Ca(2+) influx pathway of platelets and megakaryocytes
Store-operated Ca(2+) influx represents a major route by which cytosolic Ca(2+) can be elevated during platelet activation, yet its molecular identity in this cell type remains highly controversial. Using quantitative RT-PCR analysis of candidate receptor-operated cation entry pathways in human platelets, we show a 30-fold higher expression of message for ... More
Immune Correlates of Disseminated BCG Infection in IL12RB1-Deficient Mice.
Interleukin-12 receptor β1 (IL12RB1)-deficient individuals show increased susceptibilities to local or disseminated BCG infection and environmental mycobacteria infection. However, the low clinical penetrance of IL12RB1 deficiency and low recurrence rate of mycobacteria infection suggest that protective immunity still exists in this population. In this study, we investigated the mechanism of ... More
Viral Subpopulation Screening Guides in Designing a High Interferon-Inducing Live Attenuated Influenza Vaccine by Targeting Rare Mutations in NS1 and PB2 Proteins.
Influenza A viruses continue to circulate among wild birds and poultry worldwide, posing constant pandemic threats to humans. Effective control of emerging influenza viruses requires new broadly protective vaccines. Live attenuated influenza vaccines with truncations in nonstructural protein 1 (NS1) have shown broad protective efficacies in birds and mammals, which ... More
PWAR6 interacts with miR‑106a‑5p to regulate the osteogenic differentiation of human periodontal ligament stem cells.
AuthorsXiang J,Bian Y
JournalMolecular medicine reports
PubMed ID33576453
Human periodontal ligament stem cells (hPDLSCs) associated with bone regeneration serve an important role in the treatment of periodontal disease. Long non-coding RNAs are involved in the osteogenesis of multiple stem cells and can act as a sponge of microRNAs (miRs). The present study aimed to investigate the interaction between ... More
The Invasion Plasmid Antigen J (IpaJ) from Salmonella Inhibits NF-κB Activation by Suppressing IκBα Ubiquitination.
AuthorsLi Q,Xu L,Yin C,Liu Z,Li Y,Yuan Y,Hu Y,Jiao X
JournalInfection and immunity
PubMed ID31843963
Salmonella enterica serovar Pullorum is the pathogen of pullorum disease, which leads to severe economic losses in many developing countries. In contrast to the strong inflammatory response induced by Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteritidis, S. Pullorum causes systemic infection with little inflammation. The effector proteins secreted ... More
Unmasking a new prognostic marker and therapeutic target from the GDNF-RET/PIT1/p14ARF/p53 pathway in acromegaly.
BACKGROUND: Acromegaly is produced by excess growth hormone secreted by a pituitary adenoma of somatotroph cells (ACRO). First-line therapy, surgery and adjuvant therapy with somatostatin analogs, fails in 25% of patients. There is no predictive factor of resistance to therapy. New therapies are investigated using few dispersed tumor cells in ... More
GFRα 1-2-3-4 co-receptors for RET Are co-expressed in Pituitary Stem Cells but Individually Retained in Some Adenopituitary Cells.
The RET tyrosine kinase receptor is expressed by the endocrine somatotroph cells of the pituitary where it has important functions regulating survival/apoptosis. However, RET is also expressed by the GPS pituitary stem cells localized in a niche between the adenopituitary and the intermediate lobe. To bind any of its four ... More
RNA Immunoprecipitation Assay to Determine the Specificity of SRSF3 Binding to Nanog mRNA.
AuthorsRatnadiwakara M,Änkö ML
JournalBio-protocol
PubMed ID34532532
Mammalian cells express hundreds of RNA binding proteins (RBPs) that are essential regulators of RNA metabolism. RBP activity plays a central role in the control of gene expression programs and identification of RNA-protein interactions is critical for comprehensive understanding of gene regulation in cells. In recent years, various tools and ... More
Assessment of Lactobacillus rhamnosus mediated protection against arsenic-induced toxicity in zebrafish: a qPCR-based analysis of Firmicutes and Bacteroidetes groups and embryonic development.
AuthorsJain A,Jain R,Jain SK
JournalArchives of microbiology
PubMed ID37608161
KRAS and ERBB-family genetic alterations affect response to PD-1 inhibitors in metastatic nonsquamous NSCLC.
BACKGROUND: Programmed cell death 1 (PD-1) and PD-ligand 1 (PD-L1) inhibitors represent novel therapeutic options for advanced non-small cell lung cancer (NSCLC). However, approximately 50% of patients do not benefit from therapy and experience rapid disease progression. PD-L1 expression is the only approved biomarker of benefit to anti-PD-1/PD-L1 therapy. However, ... More
mRNA Stability Assay Using transcription inhibition by Actinomycin D in Mouse Pluripotent Stem Cells.
AuthorsRatnadiwakara M,Änkö ML
JournalBio-protocol
PubMed ID34532533
Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells is determined by the balance between transcription and degradation. Thus, the regulation of mRNA stability is a key post-transcriptional event that can greatly affect the net level of ... More
Ankylosis progressive homolog upregulation inhibits cell viability and mineralization during fibroblast ossification by regulating the Wnt/β‑catenin signaling pathway.
AuthorsHe X,Dong Y
JournalMolecular medicine reports
PubMed ID33173993
Ankylosis progressive homolog (ANKH) is associated with fibroblast ossification in ankylosing spondylitis (AS). As the human ANKH gene is poorly characterized relative to its murine counterpart, the aim of the present study was to examine ANKH expression in ligament tissue isolated from patients with AS and the role played by ... More
Phenotypes of STAT3 gain-of-function variant related to disruptive regulation of CXCL8/STAT3, KIT/STAT3, and IL-2/CD25/Treg axes.
Background: Vascular inflammation plays a crucial role in peripheral arterial disease (PAD), although the role of the mediators involved has not yet been properly defined. The aim of this work is to investigate gene expression and plasma biomarkers in chronic limb-threating ischemia (CLTI). Methods: Using patients from the GHAS trial, ... More
Directional X Chromosome Skewing of White Blood Cells from Subjects with Heterozygous Mosaicism for the Variant IRAK1 Haplotype.
AuthorsMorcillo P,Qin Y,Peña G,Mosenthal AC,Livingston DH,Spolarics Z
JournalInflammation
PubMed ID31748848
Long intergenic ncRNA 00473 improves the invasion of trophoblastic cells via miR-16-5p.
AuthorsWang L,Zhang J
JournalPregnancy hypertension
PubMed ID33422740
Formaldehyde-assisted isolation of regulatory DNA elements from Arabidopsis leaves.
AuthorsBaum S,Reimer-Michalski EM,Jaskiewicz MR,Conrath U
JournalNature protocols
PubMed ID32042178
An Inexpensive CRISPR-Based Point-of-Care Test for the Identification of Meat Species and Meat Products.
The growing demand for and supply of meat and meat products has led to a proportional increase in cases of meat adulteration. Adulterated meat poses serious economic and health consequences globally. Current laboratory methods for meat species identification require specialized equipment with limited field applications. This study developed an inexpensive, ... More
Ovarian transcriptome profile from pre-laying period to broody period of Xupu goose.
AuthorsQin H,Li X,Wang J,Sun G,Mu X,Ji R
JournalPoultry science
PubMed ID34425555
Xupu goose, a breed from Hunan province, produces high quality and quantity of meat and liver. However, its egg production rate is low, with poor reproductive traits but strong broody performance. These characteristics decrease the economic value of Xupu goose significantly. Here, RNA-seq was used to analyze the transcriptome changes ... More
Differential regulation of miR-146a/FAS and miR-21/FASLG axes in autoimmune lymphoproliferative syndrome due to FAS mutation (ALPS-FAS).
AuthorsMarega LF,Teocchi MA,Dos Santos Vilela MM
JournalClinical and experimental immunology
PubMed ID27060458
Most cases of autoimmune lymphoproliferative syndrome (ALPS) have an inherited genetic defect involving apoptosisârelated genes of the FAS pathway. MicroRNAs (miRNAs) are a class of small nonâcoding regulatory RNAs playing a role in the control of gene expression. This is the first report on miRNAs in ALPS patients. We studied ... More
Generation of a novel human cytomegalovirus bacterial artificial chromosome tailored for transduction of exogenous sequences.
AuthorsClose WL,Bhandari A,Hojeij M,Pellett PE
JournalVirus research
PubMed ID28912069
Expression of histone methyltransferases as novel biomarkers for renal cell tumor diagnosis and prognostication.
Renal cell tumors (RCTs) are the most lethal of the common urological cancers. The widespread use of imaging entailed an increased detection of small renal masses, emphasizing the need for accurate distinction between benign and malignant RCTs, which is critical for adequate therapeutic management. Histone methylation has been implicated in ... More
Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.
AuthorsPrince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF, Ailles LE
JournalProc Natl Acad Sci U S A
PubMed ID17210912
'Like many epithelial tumors, head and neck squamous cell carcinoma (HNSCC) contains a heterogeneous population of cancer cells. We developed an immunodeficient mouse model to test the tumorigenic potential of different populations of cancer cells derived from primary, unmanipulated human HNSCC samples. We show that a minority population of CD44(+) ... More
Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
AuthorsDagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
JournalJ Biol Chem
PubMed ID19279012
'Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy ... More
Hypoxia transcriptionally induces macrophage-inflammatory protein-3alpha/CCL-20 in primary human mononuclear phagocytes through nuclear factor (NF)-kappaB.
AuthorsBattaglia F, Delfino S, Merello E, Puppo M, Piva R, Varesio L, Bosco MC
JournalJ Leukoc Biol
PubMed ID18089854
'Hypoxia, a condition of low oxygen tension, occurring in many pathological processes, modifies the mononuclear phagocyte transcriptional profile. Here, we demonstrate hypoxic up-regulation of the CCL20 chemokine in primary human monocytes (Mn) and macrophages. mRNA induction was paralleled by protein secretion and dependent on gene transcription activation. Functional studies of ... More
Inhibition of constitutively activated nuclear factor-kappaB induces reactive oxygen species- and iron-dependent cell death in cutaneous T-cell lymphoma.
AuthorsKiessling MK, Klemke CD, Kaminski MM, Galani IE, Krammer PH, Gulow K
JournalCancer Res
PubMed ID19258503
'Aberrant signaling of the nuclear facotr (NF-kappaB) pathway has been identified as a mediator of survival and apoptosis resistance in leukemias and lymphomas. Here, we report that cell death of cutaneous T-cell lymphoma cell lines induced by inhibition of the NF-kappaB pathway is independent of caspases or classic death ... More
CNTNAP2 and NRXN1 Are Mutated in Autosomal-Recessive Pitt-Hopkins-like Mental Retardation and Determine the Level of a Common Synaptic Protein in Drosophila
AuthorsZweier, C; de Jong, EK; Zweier, M; Orrico, A; Ousager, LB; Collins, AL; Bijlsma, EK; Oortveld, MAW; Ekici, AB; Reis, A; Schenck, A; Rauch, A
Journal
PubMed ID
'Heterozygous copy-number variants and SNPs of CNTNAP2 and NRXN1, two distantly related members of the neurexin superfamily, have been repeatedly associated with a wide spectrum of neuropsychiatric disorders, such as developmental language disorders, autism spectrum disorders, epilepsy, and schizophrenia. We now identified homozygous and compound-heterozygous deletions and mutations via molecular ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
AuthorsLisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal
PubMed ID
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
Kaposi's sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implications.
AuthorsSivakumar R, Sharma-Walia N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R, Chandran B
JournalJ Virol
PubMed ID18057235
'Kaposi's sarcoma (KS), a vascular tumor associated with human immunodeficiency virus type 1 infection, is characterized by spindle-shaped endothelial cells, inflammatory cells, cytokines, growth and angiogenic factors, and angiogenesis. KS spindle cells are believed to be of the lymphatic endothelial cell (LEC) type. Kaposi's sarcoma-associated herpesvirus (KSHV, or human herpesvirus ... More
The influence of fibrin based hydrogels on the chondrogenic differentiation of human bone marrow stromal cells
AuthorsHo, STB; Cool, SM; Hui, JH; Hutmacher, DW
Journal
PubMed ID
'Mesenchymal Stem Cells (MSC) are frequently incorporated into osteochondral implants and cell seeding is often facilitated with hydrogels which exert a profound influence on the chondrogenic differentiation of MSC. An attempt was made to elucidate this effect by comparing the chondrogenic differentiation of Bone Mar-row Stromal Cells (BMSC) in fibrin ... More
The Rho guanine nucleotide exchange factor AKAP13 (BRX) is essential for cardiac development in mice.
AuthorsMayers CM, Wadell J, McLean K, Venere M, Malik M, Shibata T, Driggers PH, Kino T, Guo XC, Koide H, Gorivodsky M, Grinberg A, Mukhopadhyay M, Abu-Asab M, Westphal H, Segars JH
JournalJ Biol Chem
PubMed ID20139090
'A fundamental biologic principle is that diverse biologic signals are channeled through shared signaling cascades to regulate development. Large scaffold proteins that bind multiple proteins are capable of coordinating shared signaling pathways to provide specificity to activation of key developmental genes. Although much is known about transcription factors and target ... More
Novel epigenetic mechanisms that control pluripotency and quiescence of adult bone marrow-derived Oct4(+) very small embryonic-like stem cells
AuthorsShin, DM; Zuba-Surma, EK; Wu, W; Ratajczak, J; Wysoczynski, M; Ratajczak, MZ; Kucia, M
Journal
PubMed ID
'Recently, we identified in adult tissues a population of Oct4(+) SSEA-1(+) Sca-1(+) lin(-)CD45(-) very small embryonic-like stem cells (VSELs). First, to address recent controversies on Oct4 expression in cells isolated from adult organs, we show here evidence that Oct4 promoter in bone marrow (BM)-derived VSELs has an open chromatin structure ... More
A genetic code alteration is a phenotype diversity generator in the human pathogen Candida albicans.
AuthorsMiranda I, Rocha R, Santos MC, Mateus DD, Moura GR, Carreto L, Santos MA
JournalPlos One
PubMed ID17912373
'BACKGROUND: The discovery of genetic code alterations and expansions in both prokaryotes and eukaryotes abolished the hypothesis of a frozen and universal genetic code and exposed unanticipated flexibility in codon and amino acid assignments. It is now clear that codon identity alterations involve sense and non-sense codons and can occur ... More
Role of NF-kappaB in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-alpha.
AuthorsGauss KA, Nelson-Overton LK, Siemsen DW, Gao Y, DeLeo FR, Quinn MT
JournalJ Leukoc Biol
PubMed ID17537988
'Macrophages play an important role in the pathogenesis of chronic inflammatory disease. Activation of these phagocytes induces the production of proinflammatory cytokines, such as IL-1 and TNF-alpha and the generation of reactive oxygen species (ROS), such as superoxide anion (O2*-). Recently, we found that TNF-alpha treatment of human monocytic cells ... More
Sirtuin 1 is required for antagonist-induced transcriptional repression of androgen-responsive genes by the androgen receptor.
AuthorsDai Y, Ngo D, Forman LW, Qin DC, Jacob J, Faller DV
JournalMol Endocrinol
PubMed ID17505061
'Androgen antagonists or androgen deprivation is a primary therapeutic modality for the treatment of prostate cancer. Invariably, however, the disease becomes progressive and unresponsive to androgen ablation therapy (hormone refractory). The molecular mechanisms by which the androgen antagonists inhibit prostate cancer proliferation are not fully defined. In this report, we ... More
Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster.
AuthorsLi M, Belozerov VE, Cai HN
JournalMol Cell Biol
PubMed ID19995906
'Chromatin boundaries facilitate independent gene regulation by insulating genes from the effects of enhancers or organized chromatin. However, the mechanisms of boundary action are not well understood. To investigate whether boundary function depends on a higher order of chromatin organization, we examined the function of several Drosophila melanogaster ... More
Norwalk virus RNA is infectious in mammalian cells.
AuthorsGuix S, Asanaka M, Katayama K, Crawford SE, Neill FH, Atmar RL, Estes MK
JournalJ Virol
PubMed ID17855551
'Human noroviruses are positive-sense RNA viruses and are the leading cause of epidemic acute viral gastroenteritis in developed countries. The absence of an in vitro cell culture model for human norovirus infection has limited the development of effective antivirals and vaccines. Human histo-blood group antigens have been regarded as receptors ... More
Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny
'Background: Border disease virus (BDV) causes important reproductive losses, and eradication strategies focus on the identification and removal of persistently infected animals arising after in uterine infection. BDV infection dynamics were studied in 13 ewes experimentally infected with BDV-4 genotype at 3 phases of pregnancy [days 108 (group A), 76 ... More
β-Carotene and β-cryptoxanthin but not lutein evoke redox and immune changes in RAW264 murine macrophages
AuthorsKatsuura, S; Imamura, T; Bando, N; Yamanishi, R
Journal
PubMed ID
'The mechanism of immunological benefits induced by carotenoids has not been fully elucidated. Here, we investigated some of the immunity-related properties of β-carotene and two other carotenoids, β-cryptoxanthin, and lutein, on the murine macrophages cell line RAW264. β-Carotene added to the culture medium accumulated in the cells in a time- ... More
Interaction of Sindbis virus non-structural protein 3 with poly(ADP-ribose) polymerase 1 in neuronal cells.
AuthorsPark E, Griffin DE
JournalJ Gen Virol
PubMed ID19515826
'The alphavirus non-structural protein 3 (nsP3) has a conserved N-terminal macro domain and a variable highly phosphorylated C-terminal domain. nsP3 forms complexes with cellular proteins, but its role in virus replication is poorly understood and protein interaction domains have not been defined. As the N-terminal macro domain can bind poly(ADP-ribose) ... More
Sensitivity of the ViroSeq HIV-1 genotyping system for detection of the K103N resistance mutation in HIV-1 subtypes A, C, and D.
AuthorsChurch JD, Jones D, Flys T, Hoover D, Marlowe N, Chen S, Shi C, Eshleman JR, Guay LA, Jackson JB, Kumwenda N, Taha TE, Eshleman SH
JournalJ Mol Diagn
PubMed ID16931582
'The US Food and Drug Administration-cleared ViroSeq HIV-1 Genotyping System (ViroSeq) and other population sequencing-based human immunodeficiency virus type 1 (HIV-1) genotyping methods detect antiretroviral drug resistance mutations present in the major viral population of a test sample. These assays also detect some mutations in viral variants that are ... More
Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies
AuthorsHandy, SM; Demir, E; Hutchins, DA; Portune, KJ; Whereat, EB; Hare, CE; Rose, JM; Warner, M; Farestad, M; Cary, SC; Coyne, KJ
Journal
PubMed ID
'The Delaware Inland Bays (DIB) have experienced harmful algal blooms of dinoflagellates and raphidophytes in recent years. We used quantitative polymerase chain reaction (QPCR) techniques to investigate the community dynamics of three DIB dinoflagellates (Karlodinium veneficum, Gyrodinium instriatum, and Prorocentrum minimum) and one raphidophyte (Heterosigma akashiwo) at a single site ... More
Sakuranetin induces adipogenesis of 3T3-L1 cells through enhanced expression PPARγ2
AuthorsSaito, T; Abe, D; Sekiya, K
Journal
PubMed ID
'Sakuranetin (5,4''-dihydroxy-7-methoxyflavone) belongs to the flavanone class of polyphenols predominantly known as phytoalexin in rice plant. In this study, we demonstrate that sakuranetin strongly induces differentiation of 3T3-L1 preadipocytes, as evidenced by increased triglyceride accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity. In addition, even in the absence of adipogenic hormonal stimuli, ... More
Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa
AuthorsLi, HWR; Chiu, PCN; Cheung, MPL; Yeung, WSB; O, WS
Journal
PubMed ID
'Leptin is a polypeptide hormone with important roles in reproduction. It has been detected in human seminal plasma as well as on human ejaculated spermatozoa. This study aimed at studying the possible role of leptin in regulating human sperm functions. Immunofluorescent staining was used to study the expression of leptin ... More
Structure-Function Investigation of Vsp Serotypes of the Spirochete Borrelia hermsii
AuthorsMehra, R; Londono, D; Sondey, M; Lawson, C; Cadavid, D
Journal
PubMed ID
'Background: Relapsing fever (RF) spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. An additional role in tissue localization is suggested by the finding that isogenic serotypes 1 (Bt1) and 2 (Bt2) of the RF spirochete Borrelia turicatae, which differ only ... More
Effects of cold stress on the messenger ribonucleic acid levels of peroxisome proliferator-activated receptor-{gamma} in spleen, thymus, and bursa of Fabricius of chickens.
AuthorsWang JT, Li S, Li JL, Zhang JW, Xu SW
JournalPoult Sci
PubMed ID19903953
'This study was to investigate the expression trait of the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) gene and the effect of cold stress on the mRNA levels of PPAR-gamma in spleen, thymus, and bursa of Fabricius of chickens. Eighty-four 1-d-old male chickens were randomly allocated to 12 groups (7 chickens per group). ... More
Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts.
AuthorsOhnishi YN, Sakumi K, Yamazaki K, Ohnishi YH, Miura T, Tominaga Y, Nakabeppu Y
JournalMol Biol Cell
PubMed ID18753407
'Among fos family genes encoding components of activator protein-1 complex, only the fosB gene produces two forms of mature transcripts, namely fosB and DeltafosB mRNAs, by alternative splicing of an exonic intron. The former encodes full-length FosB. The latter encodes DeltaFosB and Delta2DeltaFosB by alternative translation initiation, and both of ... More
Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target.
AuthorsWang Y, Lee AT, Ma JZ, Wang J, Ren J, Yang Y, Tantoso E, Li KB, Ooi LL, Tan P, Lee CG
JournalJ Biol Chem
PubMed ID18319255
'Like other cancers, aberrant gene regulation features significantly in hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) were recently found to regulate gene expression at the post-transcriptional/translational levels. The expression profiles of 157 miRNAs were examined in 19 HCC patients, and 19 up-regulated and 3 down-regulated miRNAs were found to be associated with ... More
Oncogene 6b from Agrobacterium tumefaciens induces abaxial cell division at late stages of leaf development and modifies vascular development in petioles.
AuthorsTerakura S, Kitakura S, Ishikawa M, Ueno Y, Fujita T, Machida C, Wabiko H, Machida Y
JournalPlant Cell Physiol
PubMed ID16547081
'The 6b gene in the T-DNA region of the Ti plasmids of Agrobacterium tumefaciens and A. vitis is able to generate shooty calli in phytohormone-free culture of leaf sections of tobacco transformed with 6b. In the present study, we report characteristic morphological abnormalities of the leaves of transgenic tobacco and ... More
Lead Induces the Expression of Endoplasmic Reticulum Chaperones GRP78 and GRP94 in Vascular Endothelial Cells via the JNK-AP-1 Pathway.
AuthorsShinkai Y, Yamamoto C, Kaji T
JournalToxicol Sci
PubMed ID20071421
'Lead, a ubiquitous heavy metal, is an important industrial and environmental pollutant that can target the vascular endothelium. To clarify the effects of lead on the unfolded protein response (UPR) and their significance in cytotoxicity, we examined the expression and function of endoplasmic reticulum (ER) chaperones glucose-regulated protein 78 (GRP78) ... More
Delayed and acute effects of interferon-gamma on activity of an inwardly rectifying K+ channel in cultured human proximal tubule cells.
AuthorsNakamura K, Komagiri Y, Kojo T, Kubokawa M
JournalAm J Physiol Renal Physiol
PubMed ID18945831
'The activity of an inwardly rectifying K(+) channel in cultured human renal proximal tubule cells (RPTECs) is stimulated and inhibited by nitric oxide (NO) at low and high concentrations, respectively. In this study, we investigated the effects of IFN-gamma, one of the cytokines which affect the expression of inducible ... More
Plasmid DNA sequences present in conventional herpes simplex virus amplicon vectors cause rapid transgene silencing by forming inactive chromatin.
AuthorsSuzuki M, Kasai K, Saeki Y
JournalJ Virol
PubMed ID16537596
'The herpes simplex virus (HSV)-based amplicon vector, a bacterial-viral-mammalian cell shuttle system, holds promise as a versatile gene delivery vehicle because of its large transgene capacity. However, amplicon-mediated transgene expression is often transient. We hypothesized that the presence of prokaryotic DNA sequences within the packaged vector genome can trigger ... More
The Histone Demethylase JMJD2C Is Stage-Specifically Expressed in Preimplantation Mouse Embryos and Is Required for Embryonic Development
AuthorsWang, JL; Zhang, M; Zhang, Y; Kou, ZH; Han, ZM; Chen, DY; Sun, QY; Gao, SR
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PubMed ID
'Epigenetic modifications play a pivotal role in embryonic development by dynamically regulating DNA methylation and chromatin modifications. Although recent studies have shown that core histone methylation is reversible, very few studies have investigated the functions of the newly discovered histone demethylases during embryonic development. In the present study, we investigated ... More
Tryptophan-derived metabolites are required for antifungal defense in the arabidopsis mlo2 mutant.
AuthorsConsonni C, Bednarek P, Humphry M, Francocci F, Ferrari S, Harzen A, Ver Loren van Themaat E, Panstruga R
JournalPlant Physiol
PubMed ID20023151
'Arabidopsis (Arabidopsis thaliana) genes MILDEW RESISTANCE LOCUS O2 (MLO2), MLO6, and MLO12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. We show that early chlorosis and necrosis of rosette leaves in ... More
The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils.
AuthorsJacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K
JournalJ Leukoc Biol
PubMed ID18285402
'The secretory leukocyte protease inhibitor (SLPI) re-establishes homeostasis at sites of infection by virtue of its ability to exert antimicrobial activity, to suppress LPS-induced cellular immune responses, and to reduce tissue damage through inhibition of serine proteases released by polymorphonuclear neutrophil granulocytes (PMNs). Microarray analysis of bone marrow (BM) populations ... More
FCRL2 mRNA expression is inversely associated with clinical progression in chronic lymphocytic leukemia
'Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. Therefore, we investigated FCRL2 mRNA expression in a large cohort with 152 CLL patients in order to assess its role in risk prediction in B-CLL. FCRL2 mRNA expression was found ... More
Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells.
AuthorsHigashio H, Nishimura N, Ishizaki H, Miyoshi J, Orita S, Sakane A, Sasaki T
JournalJ Immunol
PubMed ID18354201
'The Doc2 family comprises the brain-specific Doc2alpha and the ubiquitous Doc2beta and Doc2gamma. With the exception of Doc2gamma, these proteins exhibit Ca(2+)-dependent phospholipid-binding activity in their Ca(2+)-binding C2A domain and are thought to be important for Ca(2+)-dependent regulated exocytosis. In excitatory neurons, Doc2alpha interacts with Munc13-1, a member of the ... More
The NIP7 protein is required for accurate pre-rRNA processing in human cells.
'Eukaryotic ribosome biogenesis requires the function of a large number of trans-acting factors which interact transiently with the nascent pre-rRNA and dissociate as the ribosomal subunits proceed to maturation and export to the cytoplasm. Loss-of-function mutations in human trans-acting factors or ribosome components may lead to genetic syndromes. In a ... More
BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate.
AuthorsJuszczynski P, Kutok JL, Li C, Mitra J, Aguiar RC, Shipp MA
JournalMol Cell Biol
PubMed ID16809771
'BAL1 is a transcription modulator that is overexpressed in chemoresistant, diffuse large B-cell lymphomas (DLBCLs). BAL1 complexes with a recently described DELTEX family member termed BBAP. Herein, we characterized BAL1 and BBAP expression in primary DLBCL subtypes defined by their comprehensive transcriptional profiles. BAL1 and BBAP were most abundant in ... More
Evidence for a network transcriptional control of promiscuous gene expression in medullary thymic epithelial cells
'The expression of peripheral tissue antigens (PTAs) in the thymus by medullary thymic epithelial cells (mTECs) is essential for the central self-tolerance in the generation of the T cell repertoire. Due to heterogeneity of autoantigen representation, this phenomenon has been termed promiscuous gene expression (PGE), in which the autoimmune regulator ... More
E3 ubiquitin ligase GRAIL controls primary T cell activation and oral tolerance.
AuthorsKriegel MA, Rathinam C, Flavell RA
JournalProc Natl Acad Sci U S A
PubMed ID19805371
'T cell unresponsiveness or anergy is one of the mechanisms that maintain inactivity of self-reactive lymphocytes. E3 ubiquitin ligases are important mediators of the anergic state. The RING finger E3 ligase GRAIL is thought to selectively function in anergic T cells but its mechanism of action and its role ... More
DNA methylation and histone modifications cause silencing of Wnt antagonist gene in human renal cell carcinoma cell lines
AuthorsKawamoto, K; Hirata, H; Kikuno, N; Tanaka, Y; Nakagawa, M; Dahiya, R
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PubMed ID
'Secreted frizzled-related protein 2 (sFRP2) is a negative modulator of the Wingless-type (Wnt) signaling pathway, and shown to be inactivated in renal cell carcinoma (RCC). However, the molecular mechanism of silencing of sFRP2 is not fully understood. Our study was designed to elucidate the silencing mechanism of sFRP2 in RCC. ... More
AuthorsKumamoto M, Nishiwaki T, Matsuo N, Kimura H, Matsushima K
JournalEur Respir J
PubMed ID19324956
'Clinical use of bone marrow mesenchymal stem cells (BMMSCs) holds great promise for regenerative medicine in intractable lung diseases, such as lung fibrosis or acute respiratory distress syndrome. However, a severe obstacle to the clinical application of BMMSC transplantation is the time-consuming, laborious processes required for cell culture. In ... More
The effects of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high-light treatment in cold conditions.
AuthorsRapacz M, Wolanin B, Hura K, Tyrka M
JournalAnn Bot
PubMed ID18245808
'BACKGROUND AND AIMS: Cold acclimation modifies the balance of the energy absorbed and metabolized in the dark processes of photosynthesis, which may affect the expression of cold-regulated (COR) genes. At the same time, a gradual acclimation to the relatively high light conditions is observed, thereby minimizing the potential for photo-oxidative ... More
The cytotoxicity of vitamin E is both vitamer- and cell-specific and involves a selectable trait.
AuthorsMcCormick CC, Parker RS
JournalJ Nutr
PubMed ID15570034
'During a study of the effect of vitamin E in activated mouse macrophages, we observed a reduction in the viability of cells treated with various forms of vitamin E. We show in this report that some tocopherols (both gamma- and delta-tocopherol) are cytotoxic to some but not all cell types. ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
AuthorsChen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
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PubMed ID
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo.
AuthorsLienenklaus S, Cornitescu M, Zietara N, Lyszkiewicz M, Gekara N, Jablonska J, Edenhofer F, Rajewsky K, Bruder D, Hafner M, Staeheli P, Weiss S
JournalJ Immunol
PubMed ID19667093
'Type I IFN is a major player in innate and adaptive immune responses. Besides, it is involved in organogenesis and tumor development. Generally, IFN responses are amplified by an autocrine loop with IFN-beta as the priming cytokine. However, due to the lack of sensitive detection systems, where and how ... More
Epigenetic alterations in RASSF1A in human aberrant crypt foci.
'CpG island methylation (CIM) is an epigenetic mechanism for transcriptional silencing that occurs at various stages of colon tumorigenesis. CIM has been found in serrated adenomas and hyperplastic polyps. There is also evidence for hypermethylation in aberrant crypt foci (ACF) that are found in resected colons from cancer patients. Our ... More
Histone deacetylases 1 and 2 form a developmental switch that controls excitatory synapse maturation and function.
AuthorsAkhtar MW, Raingo J, Nelson ED, Montgomery RL, Olson EN, Kavalali ET, Monteggia LM
JournalJ Neurosci
PubMed ID19553468
'The structural assembly of synapses can be accomplished in a rapid time frame, although most nascent synapses formed during early development are not fully functional and respond poorly to presynaptic action potentials. The mechanisms that are responsible for this delay in synapse maturation are unknown. Histone deacetylases (HDACs) regulate the ... More
A single vegetative actin isovariant overexpressed under the control of multiple regulatory sequences is sufficient for normal Arabidopsis development.
AuthorsKandasamy MK, McKinney EC, Meagher RB
JournalPlant Cell
PubMed ID19304937
'The relative significance of gene regulation and protein isovariant differences remains unexplored for most gene families, particularly those participating in multicellular development. Arabidopsis thaliana encodes three vegetative actins, ACT2, ACT7, and ACT8, in two ancient and highly divergent subclasses. Mutations in any of these differentially expressed actins revealed only ... More
Bruton's tyrosine kinase separately regulates NFkappaB p65RelA activation and cytokine interleukin (IL)-10/IL-12 production in TLR9-stimulated B Cells.
AuthorsLee KG, Xu S, Wong ET, Tergaonkar V, Lam KP
JournalJ Biol Chem
PubMed ID18276597
'B lymphocytes express both B cell receptor and Toll-like receptors (TLR). We show here that Bruton''s tyrosine kinase (Btk), a critical component in B cell receptor signaling, is also involved in TLR9 signaling in B cells. Stimulation of B cells with TLR9 ligand CpG oligodeoxynucleotide (ODN) leads to transient ... More
IL-23-dependent and -independent enhancement pathways of IL-17A production by lactic acid.
AuthorsYabu M, Shime H, Hara H, Saito T, Matsumoto M, Seya T, Akazawa T, Inoue N
JournalInt Immunol
PubMed ID21131367
'Interleukin-17A (IL-17A) is a cytokine produced by T(h)17 cells that plays an important role in inflammatory and autoimmune diseases and cancer. Stimulation with IL-6, transforming growth factor-beta , IL-21, IL-1beta and IL-23 is required for differentiation of T(h)17 cells and the production of IL-17A. Recently, we reported that tumor-derived lactic ... More
Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export.
AuthorsWoo JL, Berk AJ
JournalJ Virol
PubMed ID17079297
'Theadenovirus type 5 (Ad5) E1B-55K and E4orf6 proteins are required together to stimulate viral late nuclear mRNA export to the cytoplasm and to restrict host cell nuclear mRNA export during the late phase of infection. Previous studies have shown that these two viral proteins interact with the cellular proteins elongins ... More
Essential and overlapping functions for mammalian Argonautes in microRNA silencing.
AuthorsSu H, Trombly MI, Chen J, Wang X
JournalGenes Dev
PubMed ID19174539
'MicroRNA (miRNA) silencing fine-tunes protein output and regulates diverse biological processes. Argonaute (Ago) proteins are the core effectors of the miRNA pathway. In lower organisms, multiple Agos have evolved specialized functions for distinct RNA silencing pathways. However, the roles of mammalian Agos have not been well characterized. Here we show ... More
Cyclic AMP-dependent and Epac-mediated activation of R-Ras by G protein-coupled receptors leads to phospholipase D stimulation.
AuthorsLopez De Jesus M, Stope MB, Oude Weernink PA, Mahlke Y, Borgermann C, Ananaba VN, Rimmbach C, Rosskopf D, Michel MC, Jakobs KH, Schmidt M
JournalJ Biol Chem
PubMed ID16754664
'The activation of the Ras-related GTPase R-Ras, which has been implicated in the regulation of various cellular functions, by G protein-coupled receptors (GPCRs) was studied in HEK-293 cells stably expressing the M3 muscarinic acetylcholine receptor (mAChR), which can couple to several types of heterotrimeric G proteins. Activation of ... More
GATA transcription factors regulate the expression of the human eosinophil-derived neurotoxin (RNase 2) gene.
AuthorsQiu Z, Dyer KD, Xie Z, Radinger M, Rosenberg HF
JournalJ Biol Chem
PubMed ID19279013
'The transcription factors GATA-1 and GATA-2 have been implicated in promoting differentiation of eosinophilic leukocytes. In this study, we examined the roles of GATA-1 and GATA-2 in activating transcription of the secretory ribonuclease, the eosinophil-derived neurotoxin (EDN/RNase 2). Augmented expression of both GATA-1 and GATA-2 was detected in eosinophil ... More
FXR promotes endothelial cell motility through coordinated regulation of FAK and MMP-9.
AuthorsDas A, Yaqoob U, Mehta D, Shah VH
JournalArterioscler Thromb Vasc Biol
PubMed ID19150878
'OBJECTIVE: Farnesoid X Receptor (FXR) mediates important signaling functions of bile acids in diverse cell types including those residing in the vascular wall. Indeed, recent work has identified FXR as a potential regulator of vascular structure and function in part through transcriptional activation of MMP-9. However, the signal transduction pathways ... More
Changes in cholesterol biosynthetic and transport pathways after excitotoxicity
AuthorsKim, JH; Jittiwat, J; Ong, WY; Farooqui, AA; Jenner, AM
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PubMed ID
'The present study was carried out to elucidate changes in the gene expression and activity of cholesterol biosynthetic enzymes and transporters in the rat hippocampus after kainate excitotoxicity. Significantly increased cholesterol level was detected in the degenerating hippocampus, reaching double normal levels at 1 week after kainate injury. RT-PCR analyses ... More
Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family.
AuthorsMotoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M
JournalClin Cancer Res
PubMed ID18413822
'PURPOSE: The high mobility group A2 (HMGA2) nonhistone chromosomal protein can modulate transcription by altering chromatin architecture. HMGA2 is highly expressed during embryogenesis and in various benign and malignant tumors. Recent studies report that HMGA2 is negatively regulated by the let-7 microRNA (miRNA) family. However, no studies have examined the ... More
Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat.
AuthorsAppleford NE, Wilkinson MD, Ma Q, Evans DJ, Stone MC, Pearce SP, Powers SJ, Thomas SG, Jones HD, Phillips AL, Hedden P, Lenton JR
JournalJ Exp Bot
PubMed ID17916639
'Ectopic expression of a gibberellin 2-oxidase gene (PcGA2ox1) decreased the content of bioactive gibberellins (GAs) in transgenic wheat, producing a range of dwarf plants with different degrees of severity. In at least one case, a single transformation event gave rise to T(1) plants with different degrees of dwarfism, the phenotypes ... More
A SNP in the HSP90AA1 gene 5' flanking region is associated with the adaptation to differential thermal conditions in the ovine species
AuthorsMarcos-Carcavilla, A; Mutikainen, M; Gonzalez, C; Calvo, JH; Kantanen, J; Sanz, A; Marzanov, NS; Perez-Guzman, MD; Serrano, M
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PubMed ID
'Molecular chaperones have long been understood to be preferentially transcribed in response to multiple perturbations of the cellular homeostasis. In this study, several polymorphisms in the gene encoding the inducible form of the cytoplasmic Hsp90 (HSP90AA1) were addressed in 24 sheep breeds reared in different climatic regions of Europe, Africa, ... More
Double-stranded RNA induces shedding of the 34-kDa soluble TNFR1 from human airway epithelial cells via TLR3-TRIF-RIP1-dependent signaling: roles for dual oxidase 2- and caspase-dependent pathways.
AuthorsYu M, Lam J, Rada B, Leto TL, Levine SJ
JournalJ Immunol
PubMed ID21148036
'TNF, an important mediator of inflammatory and innate immune responses, can be regulated by binding to soluble TNF receptors. The 55-kDa type 1 TNFR (TNFR1), the key receptor for TNF signaling, is released to the extracellular space by two mechanisms, the inducible cleavage and shedding of 34-kDa soluble TNFR1 (sTNFR1) ... More
Blocking the Metabolism of Starch Breakdown Products in Arabidopsis Leaves Triggers Chloroplast Degradation
'In most plants, a large fraction of photo-assimilated carbon is stored in the chloroplasts during the day as starch and remobilized during the subsequent night to support metabolism. Mutations blocking either starch synthesis or starch breakdown in Arabidopsis thaliana reduce plant growth. Maltose is the major product of starch breakdown ... More
A novel membrane fusion-mediated plant immunity against bacterial pathogens
AuthorsHatsugai, N; Iwasaki, S; Tamura, K; Kondo, M; Fuji, K; Ogasawara, K; Nishimura, M; Hara-Nishimura, I
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PubMed ID
'Plants have developed their own defense strategies because they have no immune cells. A common plant defense strategy involves programmed cell death (PCD) at the infection site, but how the PCD-associated cell-autonomous immunity is executed in plants is not fully understood. Here we provide a novel mechanism underlying cell-autonomous immunity, ... More
Transforming growth factor-beta (TGF-beta)-inducible gene TMEPAI converts TGF-beta from a tumor suppressor to a tumor promoter in breast cancer.
AuthorsSingha PK, Yeh IT, Venkatachalam MA, Saikumar P
JournalCancer Res
PubMed ID20610632
'TMEPAI is a transforming growth factor-beta (TGF-beta)-induced transmembrane protein that is overexpressed in several cancers. How TMEPAI expression relates to malignancy is unknown. Here, we report high expression of TMEPAI in estrogen receptor/progesterone receptor-negative and human epidermal growth factor receptor-2-negative breast cancer cell lines and primary breast cancers that was ... More
PreS Deletion Mutations of Hepatitis B Virus in Chronically Infected Patients With Simultaneous Seropositivity for Hepatitis-B Surface Antigen and Anti-HBs Antibodies
'Hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (anti-HBs) may coexist in certain chronic hepatitis B (CHB) patients. This study was designed to further explore the relationship between this coexistence and hepatitis B Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg and anti-HBs (Group 1) and those ... More
Relationship of dimethylglycine, choline, and betaine with oxoproline in plasma of pregnant women and their newborn infants.
AuthorsFriesen RW, Novak EM, Hasman D, Innis SM
JournalJ Nutr
PubMed ID18029477
'Choline and glycine are inter-related through their roles in methyl metabolism. Choline is metabolized to betaine, which donates a methyl group to homocysteine to form methionine, also generating dimethylglycine, which is further metabolized to glycine. Choline is transported across the placenta and is higher in fetal than maternal plasma. Placental ... More
The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis.
AuthorsSeo PJ, Xiang F, Qiao M, Park JY, Lee YN, Kim SG, Lee YH, Park WJ, Park CM
JournalPlant Physiol
PubMed ID19625633
'Plant adaptive responses to drought are coordinated by adjusting growth and developmental processes as well as molecular and cellular activities. The root system is the primary site that perceives drought stress signals, and its development is profoundly affected by soil water content. Various growth hormones, particularly abscisic acid (ABA) and ... More
Hepatitis C Virus (HCV) Proteins Induce NADPH Oxidase 4 Expression in a Transforming Growth Factor β-Dependent Manner: a New Contributor to HCV-Induced Oxidative Stress
'Viral hepatitis-induced oxidative stress accompanied by increased levels of transforming growth factor β (TGF-β) and hepatic fibrosis are hallmarks of hepatitis C virus (HCV) infection. The mechanisms of redox regulation in the pathogenesis of HCV-induced liver disease are not clearly understood. The results of our current studies suggest that reactive ... More
Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil.
AuthorsJit S, Dadhwal M, Prakash O, Lal R
JournalInt J Syst Evol Microbiol
PubMed ID18599713
'A Gram-negative, non-spore-forming, cream-yellow-pigmented bacterial strain, IP-10(T), was isolated from soil samples from a waste site highly contaminated with hexachlorocyclohexane in Ummari village, India. The organism showed the highest 16S rRNA gene sequence similarity of 92.7 % with Flavobacterium soli KCTC 12542(T) and levels of 87-92 % with the type ... More