带有 Fast 96 孔区块模块的 7900HT Fast 实时荧光定量 PCR 系统
带有 Fast 96 孔区块模块的 7900HT Fast 实时荧光定量 PCR 系统
Applied Biosystems™

带有 Fast 96 孔区块模块的 7900HT Fast 实时荧光定量 PCR 系统

7900HT Fast 实时荧光定量 PCR 系统是一种实时定量 PCR 系统,它将 96 和 384了解更多信息
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货号 4351405
价格(CNY)
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申请报价
7900HT Fast 实时荧光定量 PCR 系统是一种实时定量 PCR 系统,它将 96 和 384 孔板的兼容性和 TaqMan™ 低密度阵列与全自动机械臂上样功能相结合,现在还提供选配的 Fast 实时荧光定量 PCR 功能。

•Fast PCR 选项将标准 96 孔规格中的运行时间缩短至约 35 分钟,或将 384 孔板中的运行时间缩短至约 55 分钟。
•500-660 nm 的连续波长检测允许在单次反应中使用多个荧光基团。
•测量基因表达水平,检测和定量测定病原体,进行等位基因分型(SNP 基因分型)分析,以及对基因序列的存在进行评分。
•96 或 384 孔板兼容性(包括 TaqMan™ 低密度阵列)。
•选配的企业版软件提供支持 21 CFR 第 11 部分指南中数据完整性和安全性相关规定的数据分析工具。
• 免手动操作板加载和卸载可提供真正无人值守的自动化,使您可以提高实验室的工作效率。
• 经证实的检测开发指南可节省时间和资金

高通量
7900HT 系统是一种检测和定量测定核酸序列的高通量实时荧光定量 PCR 系统。自动化配件与 384 孔板功能相结合,使 7900HT 系统非常适合满足当今药物发明过程的高通量要求。主要应用包括基因表达定量测定和使用荧光 5' 核酸酶试验检测单核苷酸多态性 (SNP)。

灵活性
7900HT 系统是一个通用研究工具,可以满足任何实时荧光定量 PCR 需求。用户可互换的热循环模块规格可使您选择适合您项目的规格,使用行业标准 96 和 384 孔规格以及新型 384 孔 TaqMan™ 低密度阵列和新 Fast 96 孔模块,将运行时间从 2 小时缩短至约 30 分钟。简单的自动化升级路径允许您添加功能以满足通量需求。Fast PCR 选项可将运行时间从 2 小时缩短至约 30 分钟。

功能强大的软件
使用两种自动化软件工具(板实用程序和自动化控制器),大多数基因表达和 SNP 基因分型工作流程对于高通量应用程序是完全自动化的,并且仅需很少的用户干预。您现在可以处理 5,000 个 384 孔板(384 个标志),相当于 192 万个基因型。使用相对定量测定 (RQ) 管理器,您还可以处理 200 个 384 孔板(每个多通路内源性对照具有一式四份数据点的 96 个检测器),相当于 153,600 个数据点。SNP Manager 和 RQ Manager 完全集成到一个完整的企业版系统中,通过同时分析更多的平板,突破了高通量 SNP 和基因表达研究中的数据分析瓶颈。这可减少手动操作分析时间并简化数据分析工作流程。

仅供研究使用。不适用于诊断用途。
For Research Use Only. Not for use in diagnostic procedures.
规格
已校准染料SYBR™ Green I、ROX™、NED™、JOE™、TAMRA™、VIC™、FAM™、TET™
检测方法SYBR,引物探针检测
尺寸72 cm (W) x 84 cm (D) x 64 cm (H)(抽屉打开时)
动态范围高达9个对数级线性动态范围
适用于(设备)7900HT Fast 系统
产品规格96 孔板
高通量能力兼容高通量应用,多通路(技术)
光学延长寿命 488 nm 氩离子激光激发源,无滤光片(CCD 用作光谱仪,连续检测 500-650 nm)
参比荧光染料ROX(单独管)、无 ROX、其他惰性参比染料、ROX(预混合)
数量1 instrument
反应速度快速
反应体积范围10-30 µl
运行时间33分钟(快速模式下96孔)
样品变温速率快速模式:± 2.2°C⁄s,标准模式:± 1.6°C⁄s
灵敏度低至10拷贝
温度范围(公制)4 至 100°C
热量准确度显示温度 ± 0.25°C
热循环系统基于 Peltier 的系统
热量均匀性±0.5°C
通量每天 ≥5,000 次实时 PCR 反应(384孔模块和自动化配件),每小时 ≥10,000 次终点 PCR 反应(384孔模块和自动化配件)
重量114 kg(带自动化配件),82 kg(不带自动化配件)
模块规格可互换
容量多达84个384孔板(带选配的自动化配件和384孔模块),多达36个96孔板(带选配的自动化配件)
Unit SizeEach

常见问题解答 (FAQ)

What is the threshold on the Applied Biosystems real-time PCR instruments and how do I set it?

The threshold is the numerical value assigned for each run that reflects the average dRn (change in fluorescence) during the initial cycles of PCR (baseline). The threshold is set by determining a statistically significant point (or value) above the baseline. You can manually adjust the threshold should you desire, to the place in the geometric phase where your replicates are tightest. For more information on how to manually set a threshold, please refer to the tutorial entitled Data Analysis on the ABI PRISM 7700: Setting Baselines and Thresholds (P/N 4370923). While this tutorial is based on the ABI PRISM 7700 system, the concepts are still valid for the Applied Biosystems real-time PCR instruments. You can search the document on our website by using the part number above as the keyword.

If you choose not to manually adjust the baselines and thresholds, the SDS software on the Applied Biosystems real-time PCR instruments has an Auto Ct algorithm that can be used to automatically generate baseline and threshold values for individual detectors. The algorithm calculates baseline and threshold parameters for a detector based on the assumption that the data exhibits the "typical" amplification curve. Experimental error (i.e. contamination, pipetting inaccuracies) can produce amplification curves that deviate significantly from a typical amplification curve. The data from these irregularities can affect the Auto Ct algorithm by causing it to generate incorrect baseline and threshold parameters for the associated detector. Therefore, Applied Biosystems recommends that after analysis of experimental data, you review all baseline and threshold parameters determined by the Auto Ct algorithm.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Should I run a pure dye calibration each time I exchange the sample block of the Applied Biosystems 7900HT Fast Real-Time PCR System?

You have to run a calibration when a new sample block format is used in the 7900HT Fast Real-Time PCR System, but it is not necessary to run the pure dye calibration if the block has been used before. For example, if you use a 384-well sample block for the first time, then you have to run a 384-well block calibration. Later, should you switch to a 96-well block and then switch back to a 384-well block again, then there is no need to rerun the calibration of the 384-well sample block. We recommend that you run a background run each time you exchange the sample block because dust particles or other contaminants could get into the wells while the block is being stored outside of the instrument. Please consult the 7900HT User Manual, Cat. No. 4317596, for the recommended maintenance procedures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I got an error message on the Applied Biosystems 7900HT Fast Real-Time PCR System that says to check the log file. What is this and where do I find it?

The log file tracks events during the course of an instrument run. To locate the log file, open up the hard drive of the instrument computer. Open the Applied Biosystems folder, then the SDS 2.x.x folder, then the log folder. The log file is a Microsoft Word file in .doc format and is saved as the date the run was initiated.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How do I create an indefinite hold (i.e., hold temperature at 4 degrees C forever) on the Applied Biosystems 7900HT Fast Real-Time PCR System?

To program a long-term hold on the Applied Biosystems 7900HT Fast Real-Time PCR System, launch the SDS software and open a new plate. Click on the Instrument tab. Move the cursor to the end of the thermal cycling profile, below the time box, and click. This will create a solid vertical black line. Click on “Add Hold”. Change the temperature to 4.0 and the time to the highest possible time, 98:59. Although an infinity symbol will not appear, the instrument will run for 98 hours and 59 seconds. The run must be aborted when you want to analyze the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How much heat is dissipating from the Applied Biosystems 7900HT Fast Real-Time PCR System?

While the Applied Biosystems 7900HT Fast Real-Time PCR System is idle, it puts off 6600 BTU/hour. While it is cycling, it puts off 8800 BTU/hour.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.