TaqMan™ RNase P 仪器验证板，Fast 96 孔（用于 0.1 mL 模块）

The TaqMan® RNase P Fast 96-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7500了解更多信息

货号	描述	适用于（设备）	数量
4351979	96孔	7500 Fast 系统、7900HT Fast 系统、QuantStudio™ 12k Flex、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOnePlus™、快速模式、ViiA™ 7 系统	1 块板

货号 4351979

价格（CNY)

适用于（设备）:

7500 Fast 系统、7900HT Fast 系统、QuantStudio™ 12k Flex、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOnePlus™、快速模式、ViiA™ 7 系统

The TaqMan® RNase P Fast 96-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7500 Fast, Applied Biosystems® 7900 HT Fast, StepOnePlus™, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex real-time PCR system equipped with a 96-well block. The TaqMan® RNase P Fast 96-Well Instrument Verification Plate is a sealed Optical 96-Well Fast Thermal Cycling Plate preloaded with necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template.

The TaqMan® RNase P Fast 96-Well Instrument Verification Plate satisfies the same requirements as the real-time PCR system installation. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, we recommend that the performance of your instrument be verified every six months.

仅供科研使用。不可用于诊断程序。

已校准染料FAM

校准类型RNase P 校准，仪器验证板

描述96孔

检测方法荧光

适用于（应用）实时 PCR (qPCR)

适用于（设备）7500 Fast 系统、7900HT Fast 系统、QuantStudio™ 12k Flex、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOnePlus™、快速模式、ViiA™ 7 系统

产品线TaqMan™

数量1 块板

运输条件干冰

产品类型仪器验证反应板

Unit SizeEach

内容与储存

包含一个用于 0.1 mL 模块的 96 孔快速反应板，使用 TaqMan™ 试剂（包括 RNase P 探针和引物）预加装并密封。

在 -15°C 至 -25°C 下储存。避光。

常见问题解答 (FAQ)

How can I convert a file from AQ to RQ, or from RQ to AQ file with the 7900HT Fast Real-Time PCR System?

SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.
To do so, simply go to: Tools then AQ RQ Converter
-Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
-Next click on ‘Add' and browse to the plate file location in the new window. and click ‘Open'.
-Click on ‘Convert'. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.

If you do not have v2.4, you can upgrade your software here (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html).

One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study, for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why are there no RQ values for all the samples in my study in the 7900HT Fast Real-Time PCR System software?

The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/dataassist-software.html?icid=fr-realtimesoftware-5) instead, or use the Thermo Fisher Cloud (https://www.thermofisher.com/us/en/home/cloud.html). This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any Ct or RQ values from my ddCT experiment in the 7900HT Fast Real-Time PCR System software?

In the 7900HT Fast Real-Time PCR System software, v2.3 or later, CT or RQ values are only provided in the RQ Manager software. RQ Manager is installed on the computer along with SDS v2.x.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What can I use for data analysis on the 7900HT Fast Real-Time PCR System?

For genotyping data, we recommend TaqMan Genotyper Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/taqman-genotyper-software.html?icid=fr-software-1).
For relative quantitation, we recommend ExpressionSuite Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/expressionsuite-software.html). You may also use the Thermo Fisher Cloud for either application. https://www.thermofisher.com/us/en/home/cloud.html

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What dyes can I use on the 7900HT Fast Real-Time PCR System?

The Applied Biosystems 7900HT Fast Real-Time PCR Systems use the following dye sets for calibration: FAM, SYBR, VIC, ROX, NED, TAMRA, JOE, TET dyes. Custom dyes can also be used that read between 500 nm and 660 nm, although you will have to calibrate the system first for any new dye.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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