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查看更多产品信息 Applied Biosystems™ 2720 Thermal Cycler - FAQs (4359659)
19 个常见问题解答
热循环仪的热盖经过设计可保证对微孔板施加合适的压力，以获得高效的PCR反应。使用PCR反应管时，热盖对反应管的压力过大可导致反应管变形。为避免出现这种情况，我们建议使用下述托盘/固定装置：
•货号4381850，用于Veriti系统的MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），和Veriti热循环仪、SimpliAmp热循环仪和Proflex PCR系统兼容。
•货号403081，MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），可用于7000系统、2720热循环仪和GeneAmp 9700型PCR系统。
•货号4379983，用于VeriFlex模块的MicroAmp 96孔托盘——这一托盘适用于MicroAmp联管（或MicroAmp带盖反应管，0.1 mL），可用于Veriti热循环仪、Veriti快速热循环仪、SimpliAmp热循环仪和Proflex PCR系统。
如果使用了错误的塑料耗材，或者没有使用正确的托盘/固定器，可能会发生这种情况。请查看说明书中关于正确上样的指南部分。此外，您还可以从这里(https://www.thermofisher .com/us/en/home/lifescience/pcr/pcr-plastics.html)查看兼容的塑料耗材：
Veriti热循环仪 - 上样(手册第3-5页)
ProFlex PCR系统 -上样(手册第25页)
2720热循环仪 - 上样(手册第3-5页)
请参考-2720 PCR仪专用的 实验方案指南（https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf）。
如需设置永久恒定温度，请将时间设置为“99.59”。屏幕将在此温度处默认显示（∞）图标。
没有，2720 热循环仪未整合梯度控温功能。我们的新款热循环仪 - ProFlex PCR 系统 与 Veriti 热循环仪 - 拥有超越梯度控温技术的VeriFlex模块。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。
2720 PCR仪器的最大模块变温速率是2.3 °C/秒。
可在此列表（https://www.thermofisher.com/cn/zh/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/2720-plastics.html）中选择可兼容的孔板和反应管。
2720热循环器包含一个96孔的标准模块，可以与0.2 mL的管子和孔板一起使用。
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).
Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)
Please refer to the guidelines in this protocol (https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the 2720 Thermal Cycler.
To add an infinite hold, set the time for the hold to ‘99.59'. The screen should then default to the infinity symbol for your hold.
No, the 2720 does not have a gradient feature. Our newer thermal cyclers including the ProFlex PCR System and Veriti Thermal Cycler have better-than-gradient VeriFlex blocks.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The max block ramp rate of the 2720 Thermal Cycler is 2.3 degrees C/sec.
Please see the list here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/2720-plastics.html) for compatible plates and tubes.
The 2720 Thermal Cycler contains a 96-well standard block, which can be used with 0.2 mL tubes and plates.
We recommend using the following thermal cyclers with Cytoscan HD:
For pre-PCR, we recommend 2720 Thermal Cycler (Cat. No. 4359659)
For pre- and post-PCR , we recommend the following thermal cyclers:
- GeneAmp PCR System 9700 Gold (Cat. No. 4314878) (Note: This instrument has been discontinued and therefore cannot be ordered)
- GeneAmp PCR System 9700 Silver (Cat. No. N8050001) (Note: This instrument has been discontinued and therefore cannot be ordered)
- Applied Biosystems Veriti Dx 96-Well Thermal Cycler (Cat. No. 4452300)
- Applied Biosystems Veriti 96-Well Thermal Cycler (Cat. No. 4375786)
- Bio-Rad® DNA Engine® PTC-200 Thermal Cycler
- Eppendorf® Mastercycler® Pro S Thermal Cycler
Note: You must use thermal cyclers with silver or gold-plated silver blocks. Do not use thermal cyclers with aluminum blocks.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.