Plates and Modules with Covalent Binding Surfaces, 400μL, clear, solid plate, amino - FAQs

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4 个常见问题解答

Which type of Immobilizer Amino plates and strips should I use for the different detection methods?

We recommend using transparent polystyrene plates and strips for colorimetric assays, white opaque polystyrene plates and strips for bio and chemi-luminescence assays, and black opaque polystyrene plates and strips for fluorescence assays.

Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

What advantages do your covalent binding surfaces offer over passive surfaces?

While adsorption of biomolecules to our passive surfaces is more electrostatic in nature, our CovaLink and Immobilizer Amino surfaces present an option for true covalent bonding of molecules with specific free residues. CovaLink possesses a secondary amine structure, which will covalently bond to biomolecules with a free carboxyl or phosphate group. This allows for a much more robust and specific coating, which will often tolerate more rigorous washing steps and eliminate the need for blocking.