Plates and Modules with Affinity Binding Surfaces, 350μL, C8 module and frame, clear - FAQs

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9 product FAQs found

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

Why am I getting a high background signal when using Nunc Immobilizer Streptavidin Plates/Strips?

High background signal could be due to inadequate washing. Make sure to pre-wash the Nunc Immobilizer Streptavidin Plates/Strips and increase the number of washing steps and the time between washes. Another possible cause is too high a concentration of the detection components.

How long will my Nunc Immobilizer Streptavidin Plates/Strips remain stable?

Our Nunc Immobilizer Streptavidin Plates/Strips are extremely stable over time, if stored at room temperature (20 degrees C to 25 degrees C). If kept in the unopened foil packaging, this product should have an 18 month shelf life after the date of manufacturing.

How long should I incubate the biotinylated and target molecule with the Nunc Immobilizer Streptavidin surface?

Coupling of the target molecule to the streptavidin proceeds very rapidly. Results show that incubation with gentle agitation for 2 hr at room temperature is typically sufficient to saturate all available active site.

What is the binding capacity for free biotin?

The total binding capacity for free biotin is:
- 20 pmol/well in a 96 well (coating volume 100 µL)
- 10 pmol/well in a 384 well (coating volume 50 µL)

Why should I pre-wash my Nunc Immobilizer Streptavidin?

We highly recommend pre-washing your Nunc Immobilizer Streptavidin. This will help ensure improved readouts and a very low coefficient of variation (CV% ≤ 5) from plate to plate and from well to well.

When using Nunc Immobilizer Streptavidin Plates/Strips for detection of biotinylated biomolecules, should I optimize the concentration of my target molecule?

We recommend that you optimize the amount of target molecule.
Typically, we suggest the following:
- Biotinylated oligonucleotides: 0.5 µM -0.01 µM diluted in 5 x SSCT buffer pH=7.0
- Biotinylated peptides: 1 µg/mL - 1 ng/mL diluted in a PBST buffer pH=7.2
- Biotinylated proteins: 5 µg/mL - 0.05 µg/mL diluted in a PBST buffer pH=7.2
More information regarding Nunc Immobilizer Streptavidin Plates/Strips can be found on the Thermo Scientific Nunc Immobilizer Streptavidin Instruction Protocol (link below):
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/D20693.pdf

Which types of Nunc Immobilizer Streptavidin Plates/Strips should I use for various detection methods?

We offer many types of Nunc Immobilizer Streptavidin Plates/Stripes for various applications.
We recommend:
- clear polystyrene plates and strips for colorimetric assays
- white polystyrene plates for bio- and chemiluminescence assays
- black polystyrene plates for fluorescence assays.

What types of target molecules are compatible with Nunc Immobilizer Streptavidin Plates/Strips?

When working with the Nunc Immobilizer Streptavidin instant coupling of biotinylated bio-molecules will occur. The Nunc Immobilizer Streptavidin is specifically designed and optimized for detecting biotinylated nucleic acids, peptides, and proteins.
More information regarding Nunc Immobilizer Streptavidin Plates/Strips can be found on the Thermo Scientific Nunc Immobilizer Streptavidin Instruction Protocol (link below):
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/D20693.pdf