Plates and Modules with Affinity Binding Surfaces, 400μL, solid plate, black glutathione - FAQs

5 个常见问题解答

What is the detection limit of proteins with Immobilizer Glutathione plates?

The background is extremely low when using the Immobilizer Glutathione plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).

How long are Immobilizer Glutathione plates stable?

The Immobilizer Glutathione plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.

With Immobilizer Glutathione plates, why should I use Tween-20 surfactant in the washing step and in the buffers containing the antibodies?

We recommend using 0.05% Tween-20 surfactant (as the non-ionic detergent) to improve the signal-to-noise ratio of the assay and thus decrease the background.

Should I optimize the concentration of the purified GST or GST-tagged protein to be used with Immobilizer Glutathione plates?

Yes. We recommend using a solution of the purified GST or GST-tagged protein diluted in Phosphate Buffered Saline (PBS), pH 7.2 in a concentration ranging from 0.01-1 µg/mL. During the coupling, non-ionic detergents like Tween-20 surfactant should not be present, as these will suppress the coupling.

Which type of molecules can I use with Immobilizer Glutathione plates?

The Immobilizer Glutathione plates are designed for optimal detection of glutathione-S-transferase (GST)-tagged proteins or purified GST.