Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress: Use of APE1 small molecule inhibitors to delineate APE1 functions
Oxidative DNA damage has been implicated in a number of central nervous system pathologies. The base excision repair (BER) pathway is one of the most important cellular protection mechanisms that respond to oxidative DNA damage. Human apurinic (apyrimidinic) endonuclease/redox effector factor (APE1/Ref1 or APE1) is an essential enzyme in the ... More
Characterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 and their inhibitors in equine granulosa cells in vivo and in vitro
AuthorsSessions, DR; Vick, MM; Fitzgerald, BP
JournalJOURNAL OF ANIMAL SCIENCE
PubMed ID
Matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMP) regulate tissue remodeling events necessary for ovulation. Thus, changes in MMP and TIMP expression and protein enzyme activity were examined in vivo and in vitro during follicular development and atresia in the horse. Equine granulosa cells and follicular fluid from medium ... More
The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology.
AuthorsMeyer U, Nyffeler M, Engler A, Urwyler A, Schedlowski M, Knuesel I, Yee BK, Feldon J
JournalJ Neurosci
PubMed ID16672647
Disturbance to early brain development is implicated in several neuropsychiatric disorders including autism, schizophrenia, and mental retardation. Epidemiological studies have indicated that the risk of developing these disorders is enhanced by prenatal maternal infection, presumably as a result of neurodevelopmental defects triggered by cytokine-related inflammatory events. Here, we demonstrate that ... More
Distinct roles for IFN regulatory factor (IRF)-3 and IRF-7 in the activation of antitumor properties of human macrophages.
AuthorsRomieu-Mourez R, Solis M, Nardin A, Goubau D, Baron-Bodo V, Lin R, Massie B, Salcedo M, Hiscott J
JournalCancer Res
PubMed ID17079482
When properly activated, macrophages can be tumoricidal, thus making them attractive additions to standard cancer therapies. To this end, tolerance and activity of human autologous IFN-gamma-activated macrophages, produced in large scale for clinical use (MAK cells), have been assessed in pilot trials in cancer patients. In the present study, we ... More
Molecular cloning and characterization of porcine calcineurin-alpha subunit expression in skeletal muscle.
AuthorsDepreux FF, Scheffler JM, Grant AL, Bidwell CA, Gerrard DE
JournalJ Anim Sci
PubMed ID19897633
The calmodulin/Ca(2+)-dependent serine/threonine phophatase, calcineurin (CaN), has been implicated in controlling muscle fiber phenotype. However, little information is available concerning the expression of CaN in porcine skeletal muscle. Therefore, the porcine CaN alpha (CaN-A) was cloned by reverse transcription-PCR and its expression characterized in selected porcine skeletal muscles. We successfully ... More
Trps1 plays a pivotal role downstream of Gdf5 signaling in promoting chondrogenesis and apoptosis of ATDC5 cells.
AuthorsItoh S, Kanno S, Gai Z, Suemoto H, Kawakatsu M, Tanishima H, Morimoto Y, Nishioka K, Hatamura I, Yoshida M, Muragaki Y
JournalGenes Cells
PubMed ID18363966
Tricho-rhino-phalangeal syndrome (TRPS) is an autosomal dominant skeletal disorder caused by mutations of TRPS1. Based on the similar expression patterns of Trps1 and Gdf5, we hypothesized a possible functional interaction between these two molecules. Using a chondrogenic cell line (ATDC5), we investigated the association of Gdf5-mediated signaling pathways with ... More
Cell lines as candidate reference materials for quality control of ERBB2 amplification and expression assays in breast cancer.
AuthorsXiao Y, Gao X, Maragh S, Telford WG, Tona A
JournalClin Chem
PubMed ID19443566
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) is an important biomarker whose status plays a pivotal role in therapeutic decision-making for breast cancer patients and in determining their clinical outcomes. Ensuring the accuracy and reproducibility of HER2 assays by immunohistochemistry (IHC) and by fluorescence in situ hybridization (FISH) ... More
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
AuthorsSugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
JournalJ Virol
PubMed ID19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
HIV-1 transactivator protein induction of suppressor of cytokine signaling-2 contributes to dysregulation of IFN{gamma} signaling.
AuthorsCheng SM, Li JC, Lin SS, Lee DC, Liu L, Chen Z, Lau AS
JournalBlood
PubMed ID19279332
HIV infection remains a worldwide threat. HIV-1 transactivator protein Tat is one of the retroviral proteins identified as a key immunomodulator in AIDS pathogenesis. Although the primary function of Tat is to regulate HIV-1 replication in the infected cell, it also dysregulates cytokine production resulting in perturbation of the host ... More
Intracellular HIV-Tat expression induces IL-10 synthesis by the CREB-1 transcription factor through Ser133 phosphorylation and its regulation by the ERK1/2 MAPK in human monocytic cells.
AuthorsGee K, Angel JB, Ma W, Mishra S, Gajanayaka N, Parato K, Kumar A
JournalJ Biol Chem
PubMed ID16920714
Human immunodeficiency virus (HIV)-Tat plays an important role in virus replication and in various aspects of host immune responses, including dysregulation of cytokine production. IL-10, an anti-inflammatory cytokine, is up-regulated during the course of HIV infection representing an important pathway by which HIV may induce immunodeficiency. Here we show that ... More
PURPOSE: The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in an animal model. METHODS: Retinal tissues from animals ... More
Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.
AuthorsLee JH, Tate CM, You JS, Skalnik DG
JournalJ Biol Chem
PubMed ID17355966
We previously identified a mammalian Set1A complex analogous to the yeast Set1/COMPASS histone H3-Lys4 methyltransferase complex (Lee, J.-H., and Skalnik, D. G. (2005) J. Biol. Chem. 280, 41725-41731). Data base analysis indicates that human Set1A protein shares 39% identity with an uncharacterized SET domain protein, KIAA1076, hereafter denoted ... More
Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes.
AuthorsRozema DB, Lewis DL, Wakefield DH, Wong SC, Klein JJ, Roesch PL, Bertin SL, Reppen TW, Chu Q, Blokhin AV, Hagstrom JE, Wolff JA
JournalProc Natl Acad Sci U S A
PubMed ID17652171
Achieving efficient in vivo delivery of siRNA to the appropriate target cell would be a major advance in the use of RNAi in gene function studies and as a therapeutic modality. Hepatocytes, the key parenchymal cells of the liver, are a particularly attractive target cell type for siRNA delivery ... More
A novel one-armed anti-c-Met antibody inhibits glioblastoma growth in vivo.
AuthorsMartens T, Schmidt NO, Eckerich C, Fillbrandt R, Merchant M, Schwall R, Westphal M, Lamszus K
JournalClin Cancer Res
PubMed ID17062691
PURPOSE: Expression of the receptor tyrosine kinase c-Met and its ligand scatter factor/hepatocyte growth factor (SF/HGF) are strongly increased in glioblastomas, where they promote tumor proliferation, migration, invasion, and angiogenesis. We used a novel one-armed anti-c-Met antibody to inhibit glioblastoma growth in vivo. EXPERIMENTAL DESIGN: U87 glioblastoma cells (c-Met ... More
Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina
Infection with Equid Herpesvirus type 1 (EHV-1) leads to respiratory disease, abortion, and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A(2254)/G(2254)) in the genome region of the open reading frame 30 (ORF30), which results in an amino acid variation (N(752)/D(752)) of the EHV-1 ... More
Breed difference and regulation of the porcine adipose triglyceride lipase and hormone sensitive lipase by TNFα
AuthorsShan, T; Wu, T; Reng, Y; Wang, Y
JournalANIMAL GENETICS
PubMed ID
Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are major novel triglyceride lipases in animals. The aim of this study was to determine if there are differences in the porcine ATGL (pATGL) and HSL genes between Jinhua pigs (a fatty breed) and Landrace pigs (a leaner breed). In addition, ... More
Temporal changes in bacterial rRNA and rRNA genies in Delaware (USA) coastal waters
The diversity and abundance of bacterial 16S rRNA genes (rDNA) and associated 16S rRNA were assessed to examine community structure and potential activity in a 2 yr seasonal study of marine surface waters along the Delaware (USA) coast. The majority of the ribotypes appearing as rRNA or rDNA were present ... More
Expression profiling and electrophysiological studies suggest a major role for Orai1 in the store-operated Ca(2+) influx pathway of platelets and megakaryocytes
Store-operated Ca(2+) influx represents a major route by which cytosolic Ca(2+) can be elevated during platelet activation, yet its molecular identity in this cell type remains highly controversial. Using quantitative RT-PCR analysis of candidate receptor-operated cation entry pathways in human platelets, we show a 30-fold higher expression of message for ... More
Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.
AuthorsPrince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF, Ailles LE
JournalProc Natl Acad Sci U S A
PubMed ID17210912
'Like many epithelial tumors, head and neck squamous cell carcinoma (HNSCC) contains a heterogeneous population of cancer cells. We developed an immunodeficient mouse model to test the tumorigenic potential of different populations of cancer cells derived from primary, unmanipulated human HNSCC samples. We show that a minority population of CD44(+) ... More
Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
AuthorsDagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
JournalJ Biol Chem
PubMed ID19279012
'Mitochondrial dysregulation is strongly implicated in Parkinson disease. Mutations in PTEN-induced kinase 1 (PINK1) are associated with familial parkinsonism and neuropsychiatric disorders. Although overexpressed PINK1 is neuroprotective, less is known about neuronal responses to loss of PINK1 function. We found that stable knockdown of PINK1 induced mitochondrial fragmentation and autophagy ... More
Retinoic acid modulates chromatin to potentiate tumor necrosis factor alpha signaling on the DIF2 promoter.
AuthorsWitcher M, Pettersson F, Dupere-Richer D, Padovani A, Summers-Deluca L, Baldwin AS, Miller WH Jr
JournalNucleic Acids Res
PubMed ID18039708
'Transcriptional activation by nuclear hormone receptors is well characterized, but their cooperation with other signaling pathways to activate transcription remains poorly understood. Tumor necrosis factor alpha (TNFalpha) and all-trans retinoic acid (RA) induce monocytic differentiation of acute promyelocytic leukemia (APL) cells in a synergistic manner. We used the promoter of ... More
Clinically distinct epigenetic subgroups in Silver-Russell syndrome: the degree of H19 hypomethylation associates with phenotype severity and genital and skeletal anomalies.
AuthorsBruce S, Hannula-Jouppi K, Peltonen J, Kere J, Lipsanen-Nyman M
JournalJ Clin Endocrinol Metab
PubMed ID19017756
'CONTEXT: The H19 imprinting control region (ICR), located on chromosome 11p15.5, has been reported hypomethylated in 20-65% of Silver-Russell syndrome (SRS) patients. OBJECTIVE: We investigated the methylation status of 11p15.5 ICRs in SRS patients and children born small for gestational age (SGA) to clarify the relationship between phenotype and ... More
Hypoxia transcriptionally induces macrophage-inflammatory protein-3alpha/CCL-20 in primary human mononuclear phagocytes through nuclear factor (NF)-kappaB.
AuthorsBattaglia F, Delfino S, Merello E, Puppo M, Piva R, Varesio L, Bosco MC
JournalJ Leukoc Biol
PubMed ID18089854
'Hypoxia, a condition of low oxygen tension, occurring in many pathological processes, modifies the mononuclear phagocyte transcriptional profile. Here, we demonstrate hypoxic up-regulation of the CCL20 chemokine in primary human monocytes (Mn) and macrophages. mRNA induction was paralleled by protein secretion and dependent on gene transcription activation. Functional studies of ... More
Inhibition of constitutively activated nuclear factor-kappaB induces reactive oxygen species- and iron-dependent cell death in cutaneous T-cell lymphoma.
AuthorsKiessling MK, Klemke CD, Kaminski MM, Galani IE, Krammer PH, Gulow K
JournalCancer Res
PubMed ID19258503
'Aberrant signaling of the nuclear facotr (NF-kappaB) pathway has been identified as a mediator of survival and apoptosis resistance in leukemias and lymphomas. Here, we report that cell death of cutaneous T-cell lymphoma cell lines induced by inhibition of the NF-kappaB pathway is independent of caspases or classic death ... More
CNTNAP2 and NRXN1 Are Mutated in Autosomal-Recessive Pitt-Hopkins-like Mental Retardation and Determine the Level of a Common Synaptic Protein in Drosophila
AuthorsZweier, C; de Jong, EK; Zweier, M; Orrico, A; Ousager, LB; Collins, AL; Bijlsma, EK; Oortveld, MAW; Ekici, AB; Reis, A; Schenck, A; Rauch, A
Journal
PubMed ID
'Heterozygous copy-number variants and SNPs of CNTNAP2 and NRXN1, two distantly related members of the neurexin superfamily, have been repeatedly associated with a wide spectrum of neuropsychiatric disorders, such as developmental language disorders, autism spectrum disorders, epilepsy, and schizophrenia. We now identified homozygous and compound-heterozygous deletions and mutations via molecular ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
AuthorsLisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal
PubMed ID
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
Kaposi's sarcoma-associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological implications.
AuthorsSivakumar R, Sharma-Walia N, Raghu H, Veettil MV, Sadagopan S, Bottero V, Varga L, Levine R, Chandran B
JournalJ Virol
PubMed ID18057235
'Kaposi's sarcoma (KS), a vascular tumor associated with human immunodeficiency virus type 1 infection, is characterized by spindle-shaped endothelial cells, inflammatory cells, cytokines, growth and angiogenic factors, and angiogenesis. KS spindle cells are believed to be of the lymphatic endothelial cell (LEC) type. Kaposi's sarcoma-associated herpesvirus (KSHV, or human herpesvirus ... More
Blockade of the ubiquitin protease UBP43 destabilizes transcription factor PML/RARalpha and inhibits the growth of acute promyelocytic leukemia.
AuthorsGuo Y, Dolinko AV, Chinyengetere F, Stanton B, Bomberger JM, Demidenko E, Zhou DC, Gallagher R, Ma T, Galimberti F, Liu X, Sekula D, Freemantle S, Dmitrovsky E
JournalCancer Res
PubMed ID20935222
'More effective treatments for acute promyelocytic leukemia (APL) are needed. APL cell treatment with all-trans-retinoic acid (RA) degrades the chimeric, dominant-negative-acting transcription factor promyelocytic leukemia gene (PML)/RARalpha, which is generated in APL by chromosomal translocation. The E1-like ubiquitin-activating enzyme (UBE1L) associates with interferon-stimulated gene ISG15 that binds and represses PML/RARalpha ... More
The influence of fibrin based hydrogels on the chondrogenic differentiation of human bone marrow stromal cells
AuthorsHo, STB; Cool, SM; Hui, JH; Hutmacher, DW
Journal
PubMed ID
'Mesenchymal Stem Cells (MSC) are frequently incorporated into osteochondral implants and cell seeding is often facilitated with hydrogels which exert a profound influence on the chondrogenic differentiation of MSC. An attempt was made to elucidate this effect by comparing the chondrogenic differentiation of Bone Mar-row Stromal Cells (BMSC) in fibrin ... More
The Rho guanine nucleotide exchange factor AKAP13 (BRX) is essential for cardiac development in mice.
AuthorsMayers CM, Wadell J, McLean K, Venere M, Malik M, Shibata T, Driggers PH, Kino T, Guo XC, Koide H, Gorivodsky M, Grinberg A, Mukhopadhyay M, Abu-Asab M, Westphal H, Segars JH
JournalJ Biol Chem
PubMed ID20139090
'A fundamental biologic principle is that diverse biologic signals are channeled through shared signaling cascades to regulate development. Large scaffold proteins that bind multiple proteins are capable of coordinating shared signaling pathways to provide specificity to activation of key developmental genes. Although much is known about transcription factors and target ... More
Novel epigenetic mechanisms that control pluripotency and quiescence of adult bone marrow-derived Oct4(+) very small embryonic-like stem cells
AuthorsShin, DM; Zuba-Surma, EK; Wu, W; Ratajczak, J; Wysoczynski, M; Ratajczak, MZ; Kucia, M
Journal
PubMed ID
'Recently, we identified in adult tissues a population of Oct4(+) SSEA-1(+) Sca-1(+) lin(-)CD45(-) very small embryonic-like stem cells (VSELs). First, to address recent controversies on Oct4 expression in cells isolated from adult organs, we show here evidence that Oct4 promoter in bone marrow (BM)-derived VSELs has an open chromatin structure ... More
A genetic code alteration is a phenotype diversity generator in the human pathogen Candida albicans.
AuthorsMiranda I, Rocha R, Santos MC, Mateus DD, Moura GR, Carreto L, Santos MA
JournalPlos One
PubMed ID17912373
'BACKGROUND: The discovery of genetic code alterations and expansions in both prokaryotes and eukaryotes abolished the hypothesis of a frozen and universal genetic code and exposed unanticipated flexibility in codon and amino acid assignments. It is now clear that codon identity alterations involve sense and non-sense codons and can occur ... More
Role of NF-kappaB in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-alpha.
AuthorsGauss KA, Nelson-Overton LK, Siemsen DW, Gao Y, DeLeo FR, Quinn MT
JournalJ Leukoc Biol
PubMed ID17537988
'Macrophages play an important role in the pathogenesis of chronic inflammatory disease. Activation of these phagocytes induces the production of proinflammatory cytokines, such as IL-1 and TNF-alpha and the generation of reactive oxygen species (ROS), such as superoxide anion (O2*-). Recently, we found that TNF-alpha treatment of human monocytic cells ... More
Inhibition of cyclooxygenase-2 suppresses lymph node metastasis via reduction of lymphangiogenesis.
AuthorsIwata C, Kano MR, Komuro A, Oka M, Kiyono K, Johansson E, Morishita Y, Yashiro M, Hirakawa K, Kaminishi M, Miyazono K
JournalCancer Res
PubMed ID17974958
'Cyclooxygenase-2 (COX-2) inhibitor has been reported to suppress tumor progression. However, it is unclear whether this inhibitor can also prevent lymphatic metastasis. To determine the effects of COX-2 inhibitor on lymphatic metastasis, etodolac, a COX-2 inhibitor, was given p.o. to mice bearing orthotopic xenografts or with carcinomatous peritonitis induced with ... More
Sirtuin 1 is required for antagonist-induced transcriptional repression of androgen-responsive genes by the androgen receptor.
AuthorsDai Y, Ngo D, Forman LW, Qin DC, Jacob J, Faller DV
JournalMol Endocrinol
PubMed ID17505061
'Androgen antagonists or androgen deprivation is a primary therapeutic modality for the treatment of prostate cancer. Invariably, however, the disease becomes progressive and unresponsive to androgen ablation therapy (hormone refractory). The molecular mechanisms by which the androgen antagonists inhibit prostate cancer proliferation are not fully defined. In this report, we ... More
Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster.
AuthorsLi M, Belozerov VE, Cai HN
JournalMol Cell Biol
PubMed ID19995906
'Chromatin boundaries facilitate independent gene regulation by insulating genes from the effects of enhancers or organized chromatin. However, the mechanisms of boundary action are not well understood. To investigate whether boundary function depends on a higher order of chromatin organization, we examined the function of several Drosophila melanogaster ... More
MicroRNA-223 regulates Glut4 expression and cardiomyocyte glucose metabolism.
AuthorsLu H, Buchan RJ, Cook SA
JournalCardiovasc Res
PubMed ID20080987
'AIMS: MicroRNAs (miRNAs) are important for cardiac function and tissue metabolism. The aim of the present study is to investigate the role(s) of miRNAs in the insulin-resistant heart. METHODS AND RESULTS: Left ventricular biopsies were collected from patients with or without type 2 diabetes and from patients with left ... More
Norwalk virus RNA is infectious in mammalian cells.
AuthorsGuix S, Asanaka M, Katayama K, Crawford SE, Neill FH, Atmar RL, Estes MK
JournalJ Virol
PubMed ID17855551
'Human noroviruses are positive-sense RNA viruses and are the leading cause of epidemic acute viral gastroenteritis in developed countries. The absence of an in vitro cell culture model for human norovirus infection has limited the development of effective antivirals and vaccines. Human histo-blood group antigens have been regarded as receptors ... More
Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
AuthorsGillan S, O'Brien R, Hughes AD, Griffin JF
JournalClin Vaccine Immunol
PubMed ID19923568
'Johne''s disease, a chronic enteritis of ruminants, is caused by infection with Mycobacterium avium subsp. paratuberculosis. Three distinct forms have been observed in sheep: paucibacillary disease (PB), multibacillary disease (MB), and asymptomatic infection (AS). In this study, immune parameters for animals naturally infected with M. avium subsp. paratuberculosis and ... More
Dependence of stress resistance on a spore coat heteropolysaccharide in Dictyostelium.
AuthorsWest CM, Nguyen P, van der Wel H, Metcalf T, Sweeney KR, Blader IJ, Erdos GW
JournalEukaryot Cell
PubMed ID18996984
'In Dictyostelium, sporulation occurs synchronously as prespore cells approach the apex of the aerial stalk during culmination. Each prespore cell becomes surrounded by its own coat comprised of a core of crystalline cellulose and a branched heteropolysaccharide sandwiched between heterogeneous cysteine-rich glycoproteins. The function of the heteropolysaccharide, which consists of ... More
Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny
'Background: Border disease virus (BDV) causes important reproductive losses, and eradication strategies focus on the identification and removal of persistently infected animals arising after in uterine infection. BDV infection dynamics were studied in 13 ewes experimentally infected with BDV-4 genotype at 3 phases of pregnancy [days 108 (group A), 76 ... More
β-Carotene and β-cryptoxanthin but not lutein evoke redox and immune changes in RAW264 murine macrophages
AuthorsKatsuura, S; Imamura, T; Bando, N; Yamanishi, R
Journal
PubMed ID
'The mechanism of immunological benefits induced by carotenoids has not been fully elucidated. Here, we investigated some of the immunity-related properties of β-carotene and two other carotenoids, β-cryptoxanthin, and lutein, on the murine macrophages cell line RAW264. β-Carotene added to the culture medium accumulated in the cells in a time- ... More
Interaction of Sindbis virus non-structural protein 3 with poly(ADP-ribose) polymerase 1 in neuronal cells.
AuthorsPark E, Griffin DE
JournalJ Gen Virol
PubMed ID19515826
'The alphavirus non-structural protein 3 (nsP3) has a conserved N-terminal macro domain and a variable highly phosphorylated C-terminal domain. nsP3 forms complexes with cellular proteins, but its role in virus replication is poorly understood and protein interaction domains have not been defined. As the N-terminal macro domain can bind poly(ADP-ribose) ... More
Sensitivity of the ViroSeq HIV-1 genotyping system for detection of the K103N resistance mutation in HIV-1 subtypes A, C, and D.
AuthorsChurch JD, Jones D, Flys T, Hoover D, Marlowe N, Chen S, Shi C, Eshleman JR, Guay LA, Jackson JB, Kumwenda N, Taha TE, Eshleman SH
JournalJ Mol Diagn
PubMed ID16931582
'The US Food and Drug Administration-cleared ViroSeq HIV-1 Genotyping System (ViroSeq) and other population sequencing-based human immunodeficiency virus type 1 (HIV-1) genotyping methods detect antiretroviral drug resistance mutations present in the major viral population of a test sample. These assays also detect some mutations in viral variants that are ... More
Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies
AuthorsHandy, SM; Demir, E; Hutchins, DA; Portune, KJ; Whereat, EB; Hare, CE; Rose, JM; Warner, M; Farestad, M; Cary, SC; Coyne, KJ
Journal
PubMed ID
'The Delaware Inland Bays (DIB) have experienced harmful algal blooms of dinoflagellates and raphidophytes in recent years. We used quantitative polymerase chain reaction (QPCR) techniques to investigate the community dynamics of three DIB dinoflagellates (Karlodinium veneficum, Gyrodinium instriatum, and Prorocentrum minimum) and one raphidophyte (Heterosigma akashiwo) at a single site ... More
AuthorsSaito RA, Watabe T, Horiguchi K, Kohyama T, Saitoh M, Nagase T, Miyazono K
JournalCancer Res
PubMed ID19293183
'Thyroid transcription factor-1 (TTF-1) is expressed in lung cancer, but its functional roles remain unexplored. TTF-1 gene amplification has been discovered in a part of lung adenocarcinomas, and its action as a lineage-specific oncogene is highlighted. Epithelial-to-mesenchymal transition (EMT) is a crucial event for cancer cells to acquire ... More
Sakuranetin induces adipogenesis of 3T3-L1 cells through enhanced expression PPARγ2
AuthorsSaito, T; Abe, D; Sekiya, K
Journal
PubMed ID
'Sakuranetin (5,4''-dihydroxy-7-methoxyflavone) belongs to the flavanone class of polyphenols predominantly known as phytoalexin in rice plant. In this study, we demonstrate that sakuranetin strongly induces differentiation of 3T3-L1 preadipocytes, as evidenced by increased triglyceride accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity. In addition, even in the absence of adipogenic hormonal stimuli, ... More
Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates.
AuthorsLausten-Thomsen U, Madsen HO, Vestergaard TR, Hjalgrim H, Nersting J, Schmiegelow K
JournalBlood
PubMed ID20713965
't(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood ... More
Development and validation of a multiplex real-time PCR assay for simultaneous genotyping and human T-lymphotropic virus type 1, 2, and 3 proviral load determination.
AuthorsMoens B, Lopez G, Adaui V, Gonzalez E, Kerremans L, Clark D, Verdonck K, Gotuzzo E, Vanham G, Cassar O, Gessain A, Vandamme AM, Van Dooren S
JournalJ Clin Microbiol
PubMed ID19741085
'The human T-lymphotropic virus (HTLV) proviral load remains the best surrogate marker for disease progression. Real-time PCR techniques have been developed for detection and quantification of cosmopolitan HTLV type 1a (HTLV-1a) and HTLV-2. Since a growing level of diversity in subtypes and genotypes is observed, we developed a multiplex ... More
Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa
AuthorsLi, HWR; Chiu, PCN; Cheung, MPL; Yeung, WSB; O, WS
Journal
PubMed ID
'Leptin is a polypeptide hormone with important roles in reproduction. It has been detected in human seminal plasma as well as on human ejaculated spermatozoa. This study aimed at studying the possible role of leptin in regulating human sperm functions. Immunofluorescent staining was used to study the expression of leptin ... More
von Hippel-Lindau protein regulates transition from the fetal to the adult circulatory system in retina.
AuthorsKurihara T, Kubota Y, Ozawa Y, Takubo K, Noda K, Simon MC, Johnson RS, Suematsu M, Tsubota K, Ishida S, Goda N, Suda T, Okano H
JournalDevelopment
PubMed ID20388654
'In early neonates, the fetal circulatory system undergoes dramatic transition to the adult circulatory system. Normally, embryonic connecting vessels, such as the ductus arteriosus and the foramen ovale, close and regress. In the neonatal retina, hyaloid vessels maintaining blood flow in the embryonic retina regress, and retinal vessels take over ... More
Structure-Function Investigation of Vsp Serotypes of the Spirochete Borrelia hermsii
AuthorsMehra, R; Londono, D; Sondey, M; Lawson, C; Cadavid, D
Journal
PubMed ID
'Background: Relapsing fever (RF) spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. An additional role in tissue localization is suggested by the finding that isogenic serotypes 1 (Bt1) and 2 (Bt2) of the RF spirochete Borrelia turicatae, which differ only ... More
Activation of paternally expressed genes and perinatal death caused by deletion of the Gtl2 gene.
AuthorsZhou Y, Cheunsuchon P, Nakayama Y, Lawlor MW, Zhong Y, Rice KA, Zhang L, Zhang X, Gordon FE, Lidov HG, Bronson RT, Klibanski A
JournalDevelopment
PubMed ID20610486
'The Dlk1-Gtl2 imprinting locus is located on mouse distal chromosome 12 and consists of multiple maternally expressed non-coding RNAs and several paternally expressed protein-coding genes. The imprinting of this locus plays a crucial role in embryonic development and postnatal growth. At least one cis-element, the intergenic differentially methylated region (IG-DMR) ... More
Effects of cold stress on the messenger ribonucleic acid levels of peroxisome proliferator-activated receptor-{gamma} in spleen, thymus, and bursa of Fabricius of chickens.
AuthorsWang JT, Li S, Li JL, Zhang JW, Xu SW
JournalPoult Sci
PubMed ID19903953
'This study was to investigate the expression trait of the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) gene and the effect of cold stress on the mRNA levels of PPAR-gamma in spleen, thymus, and bursa of Fabricius of chickens. Eighty-four 1-d-old male chickens were randomly allocated to 12 groups (7 chickens per group). ... More
Antagonistic regulation of cell-matrix adhesion by FosB and DeltaFosB/Delta2DeltaFosB encoded by alternatively spliced forms of fosB transcripts.
AuthorsOhnishi YN, Sakumi K, Yamazaki K, Ohnishi YH, Miura T, Tominaga Y, Nakabeppu Y
JournalMol Biol Cell
PubMed ID18753407
'Among fos family genes encoding components of activator protein-1 complex, only the fosB gene produces two forms of mature transcripts, namely fosB and DeltafosB mRNAs, by alternative splicing of an exonic intron. The former encodes full-length FosB. The latter encodes DeltaFosB and Delta2DeltaFosB by alternative translation initiation, and both of ... More
Genome-wide analysis of mRNA targets for Caenorhabditis elegans FBF, a conserved stem cell regulator.
AuthorsKershner AM, Kimble J
JournalProc Natl Acad Sci U S A
PubMed ID20142496
'Stem cells are essential for tissue generation during the development of multicellular creatures, and for tissue homeostasis in adults. The great therapeutic promise of stem cells makes understanding their regulation a high priority. PUF RNA-binding proteins have a conserved role in promoting self-renewal of germline stem cells. Here we use ... More
Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target.
AuthorsWang Y, Lee AT, Ma JZ, Wang J, Ren J, Yang Y, Tantoso E, Li KB, Ooi LL, Tan P, Lee CG
JournalJ Biol Chem
PubMed ID18319255
'Like other cancers, aberrant gene regulation features significantly in hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) were recently found to regulate gene expression at the post-transcriptional/translational levels. The expression profiles of 157 miRNAs were examined in 19 HCC patients, and 19 up-regulated and 3 down-regulated miRNAs were found to be associated with ... More
Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species.
AuthorsAmmor MS, Florez AB, Alvarez-Martin P, Margolles A, Mayo B
JournalJ Antimicrob Chemother
PubMed ID18614524
'OBJECTIVES: The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria ... More
Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells.
AuthorsMaubach G, Lim MC, Zhuo L
JournalMol Biol Cell
PubMed ID18667530
'Activation of hepatic stellate cells during liver fibrosis is a major event facilitating an increase in extracellular matrix deposition. The up-regulation of smooth muscle alpha-actin and collagen type I is indicative of the activation process. The involvement of cysteine cathepsins, a class of lysosomal cysteine proteases, has not been studied ... More
Oncogene 6b from Agrobacterium tumefaciens induces abaxial cell division at late stages of leaf development and modifies vascular development in petioles.
AuthorsTerakura S, Kitakura S, Ishikawa M, Ueno Y, Fujita T, Machida C, Wabiko H, Machida Y
JournalPlant Cell Physiol
PubMed ID16547081
'The 6b gene in the T-DNA region of the Ti plasmids of Agrobacterium tumefaciens and A. vitis is able to generate shooty calli in phytohormone-free culture of leaf sections of tobacco transformed with 6b. In the present study, we report characteristic morphological abnormalities of the leaves of transgenic tobacco and ... More
Lead Induces the Expression of Endoplasmic Reticulum Chaperones GRP78 and GRP94 in Vascular Endothelial Cells via the JNK-AP-1 Pathway.
AuthorsShinkai Y, Yamamoto C, Kaji T
JournalToxicol Sci
PubMed ID20071421
'Lead, a ubiquitous heavy metal, is an important industrial and environmental pollutant that can target the vascular endothelium. To clarify the effects of lead on the unfolded protein response (UPR) and their significance in cytotoxicity, we examined the expression and function of endoplasmic reticulum (ER) chaperones glucose-regulated protein 78 (GRP78) ... More
Delayed and acute effects of interferon-gamma on activity of an inwardly rectifying K+ channel in cultured human proximal tubule cells.
AuthorsNakamura K, Komagiri Y, Kojo T, Kubokawa M
JournalAm J Physiol Renal Physiol
PubMed ID18945831
'The activity of an inwardly rectifying K(+) channel in cultured human renal proximal tubule cells (RPTECs) is stimulated and inhibited by nitric oxide (NO) at low and high concentrations, respectively. In this study, we investigated the effects of IFN-gamma, one of the cytokines which affect the expression of inducible ... More
Long-term administration of estradiol decreases expression of hepatic lipogenic genes and improves insulin sensitivity in ob/ob mice: a possible mechanism is through direct regulation of signal transducer and activator of transcription 3.
AuthorsGao H, Bryzgalova G, Hedman E, Khan A, Efendic S, Gustafsson JA, Dahlman-Wright K
JournalMol Endocrinol
PubMed ID16627594
'In this study, we used ob/ob mice as a model to investigate the effects of long-term estradiol administration on insulin sensitivity and to explore the mechanisms that underlie the antidiabetic effects of estrogen on mouse liver. Female ob/ob mice were randomly divided into two groups and given estradiol (100 ... More
Differential regulation of dopaminergic gene expression by Er81.
AuthorsCave JW, Akiba Y, Banerjee K, Bhosle S, Berlin R, Baker H
JournalJ Neurosci
PubMed ID20357122
'A recent study proposed that differentiation of dopaminergic neurons requires a conserved "dopamine motif" (DA-motif) that functions as a binding site for ETS DNA binding domain transcription factors. In the mammalian olfactory bulb (OB), the expression of a set of five genes [including tyrosine hydroxylase (Th)] that are necessary for ... More
Interleukin-10 attenuates vascular responses to endothelin-1 via effects on ERK1/2-dependent pathway.
AuthorsGiachini FR, Zemse SM, Carneiro FS, Lima VV, Carneiro ZN, Callera GE, Ergul A, Webb RC, Tostes RC
JournalAm J Physiol Heart Circ Physiol
PubMed ID19074677
'Interleukin-10 (IL-10) is an anti-inflammatory cytokine with protective actions on the vasculature. On the other hand, endothelin (ET)-1 has potent vasoconstrictor, mitogenic, and proinflammatory activities, which have been implicated in the pathophysiology of a number of cardiovascular diseases. We hypothesized that, in a condition where ET-1 expression is upregulated, i.e., ... More
Plasmid DNA sequences present in conventional herpes simplex virus amplicon vectors cause rapid transgene silencing by forming inactive chromatin.
AuthorsSuzuki M, Kasai K, Saeki Y
JournalJ Virol
PubMed ID16537596
'The herpes simplex virus (HSV)-based amplicon vector, a bacterial-viral-mammalian cell shuttle system, holds promise as a versatile gene delivery vehicle because of its large transgene capacity. However, amplicon-mediated transgene expression is often transient. We hypothesized that the presence of prokaryotic DNA sequences within the packaged vector genome can trigger ... More
The Histone Demethylase JMJD2C Is Stage-Specifically Expressed in Preimplantation Mouse Embryos and Is Required for Embryonic Development
AuthorsWang, JL; Zhang, M; Zhang, Y; Kou, ZH; Han, ZM; Chen, DY; Sun, QY; Gao, SR
Journal
PubMed ID
'Epigenetic modifications play a pivotal role in embryonic development by dynamically regulating DNA methylation and chromatin modifications. Although recent studies have shown that core histone methylation is reversible, very few studies have investigated the functions of the newly discovered histone demethylases during embryonic development. In the present study, we investigated ... More
Environmentally relevant concentrations of 17alpha-ethinylestradiol (EE2) interfere with the growth hormone (GH)/insulin-like growth factor (IGF)-I system in developing bony fish.
AuthorsShved N, Berishvili G, Baroiller JF, Segner H, Reinecke M
JournalToxicol Sci
PubMed ID18660547
'The aim of this study was to evaluate whether effects of environmental estrogens on fish growth and reproduction may be mediated via modulating the growth hormone (GH)/insulin-like growth factor I (IGF-I) system. To this end, developing male and female monosex populations of tilapia were exposed to 17alpha-ethinylestradiol (EE2) at ... More
Expression of hepatitis C virus (HCV) structural proteins in trans facilitates encapsidation and transmission of HCV subgenomic RNA.
'A characteristic of many positive-strand RNA viruses is that, whilst replication of the viral genome is dependent on the expression of the majority of non-structural proteins in cis, virus particle formation can occur when most or all of the structural proteins are co-expressed in trans. Making use of a ... More
Tryptophan-derived metabolites are required for antifungal defense in the arabidopsis mlo2 mutant.
AuthorsConsonni C, Bednarek P, Humphry M, Francocci F, Ferrari S, Harzen A, Ver Loren van Themaat E, Panstruga R
JournalPlant Physiol
PubMed ID20023151
'Arabidopsis (Arabidopsis thaliana) genes MILDEW RESISTANCE LOCUS O2 (MLO2), MLO6, and MLO12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. We show that early chlorosis and necrosis of rosette leaves in ... More
The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils.
AuthorsJacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K
JournalJ Leukoc Biol
PubMed ID18285402
'The secretory leukocyte protease inhibitor (SLPI) re-establishes homeostasis at sites of infection by virtue of its ability to exert antimicrobial activity, to suppress LPS-induced cellular immune responses, and to reduce tissue damage through inhibition of serine proteases released by polymorphonuclear neutrophil granulocytes (PMNs). Microarray analysis of bone marrow (BM) populations ... More
FCRL2 mRNA expression is inversely associated with clinical progression in chronic lymphocytic leukemia
'Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. Therefore, we investigated FCRL2 mRNA expression in a large cohort with 152 CLL patients in order to assess its role in risk prediction in B-CLL. FCRL2 mRNA expression was found ... More
Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells.
AuthorsHigashio H, Nishimura N, Ishizaki H, Miyoshi J, Orita S, Sakane A, Sasaki T
JournalJ Immunol
PubMed ID18354201
'The Doc2 family comprises the brain-specific Doc2alpha and the ubiquitous Doc2beta and Doc2gamma. With the exception of Doc2gamma, these proteins exhibit Ca(2+)-dependent phospholipid-binding activity in their Ca(2+)-binding C2A domain and are thought to be important for Ca(2+)-dependent regulated exocytosis. In excitatory neurons, Doc2alpha interacts with Munc13-1, a member of the ... More
Suppressor of cytokine signalling-3 is up-regulated by androgen in prostate cancer cell lines and inhibits androgen-mediated proliferation and secretion.
AuthorsNeuwirt H, Puhr M, Cavarretta IT, Mitterberger M, Hobisch A, Culig Z
JournalEndocr Relat Cancer
PubMed ID18045952
'Suppressors of cytokine signalling (SOCS) are induced by interleukins (ILs) and various peptide hormones and may prevent sustained activation of signalling pathways. We have previously shown that SOCS-3 antagonizes regulation of cellular events by cAMP and is expressed in human prostate cancer. To investigate possible effects of androgen on SOCS-3 ... More
Generation of mice transgenic for human CYP2C18 and CYP2C19: characterization of the sexually dimorphic gene and enzyme expression.
AuthorsLofgren S, Baldwin RM, Hiratsuka M, Lindqvist A, Carlberg A, Sim SC, Schulke M, Snait M, Edenro A, Fransson-Steen R, Terelius Y, Ingelman-Sundberg M
JournalDrug Metab Dispos
PubMed ID18276835
'CYP2C19 is an important enzyme for human drug metabolism, and it also participates in the metabolism of endogenous substrates, whereas the CYP2C18 enzyme is not expressed in human liver despite high mRNA expression. Mice transgenic for the human CYP2C18 and CYP2C19 genes were generated. Quantitative mRNA analysis showed CYP2C18 and ... More
Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds for antipoliovirus activity.
AuthorsArita M, Kojima H, Nagano T, Okabe T, Wakita T, Shimizu H
JournalJ Virol
PubMed ID21177810
'Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding region causing resistance to enviroxime ... More
The NIP7 protein is required for accurate pre-rRNA processing in human cells.
'Eukaryotic ribosome biogenesis requires the function of a large number of trans-acting factors which interact transiently with the nascent pre-rRNA and dissociate as the ribosomal subunits proceed to maturation and export to the cytoplasm. Loss-of-function mutations in human trans-acting factors or ribosome components may lead to genetic syndromes. In a ... More
BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate.
AuthorsJuszczynski P, Kutok JL, Li C, Mitra J, Aguiar RC, Shipp MA
JournalMol Cell Biol
PubMed ID16809771
'BAL1 is a transcription modulator that is overexpressed in chemoresistant, diffuse large B-cell lymphomas (DLBCLs). BAL1 complexes with a recently described DELTEX family member termed BBAP. Herein, we characterized BAL1 and BBAP expression in primary DLBCL subtypes defined by their comprehensive transcriptional profiles. BAL1 and BBAP were most abundant in ... More
Evidence for a network transcriptional control of promiscuous gene expression in medullary thymic epithelial cells
'The expression of peripheral tissue antigens (PTAs) in the thymus by medullary thymic epithelial cells (mTECs) is essential for the central self-tolerance in the generation of the T cell repertoire. Due to heterogeneity of autoantigen representation, this phenomenon has been termed promiscuous gene expression (PGE), in which the autoimmune regulator ... More
E3 ubiquitin ligase GRAIL controls primary T cell activation and oral tolerance.
AuthorsKriegel MA, Rathinam C, Flavell RA
JournalProc Natl Acad Sci U S A
PubMed ID19805371
'T cell unresponsiveness or anergy is one of the mechanisms that maintain inactivity of self-reactive lymphocytes. E3 ubiquitin ligases are important mediators of the anergic state. The RING finger E3 ligase GRAIL is thought to selectively function in anergic T cells but its mechanism of action and its role ... More
Regulation of soluble guanylyl cyclase-alpha1 expression in chronic hypoxia-induced pulmonary hypertension: role of NFATc3 and HuR.
'The nitric oxide/soluble guanylyl cyclase (sGC) signal transduction pathway plays an important role in smooth muscle relaxation and phenotypic regulation. However, the transcriptional regulation of sGC gene expression is largely unknown. It has been shown that sGC expression increases in pulmonary arteries from chronic hypoxia-induced pulmonary hypertensive animals. Since ... More
DNA methylation and histone modifications cause silencing of Wnt antagonist gene in human renal cell carcinoma cell lines
AuthorsKawamoto, K; Hirata, H; Kikuno, N; Tanaka, Y; Nakagawa, M; Dahiya, R
Journal
PubMed ID
'Secreted frizzled-related protein 2 (sFRP2) is a negative modulator of the Wingless-type (Wnt) signaling pathway, and shown to be inactivated in renal cell carcinoma (RCC). However, the molecular mechanism of silencing of sFRP2 is not fully understood. Our study was designed to elucidate the silencing mechanism of sFRP2 in RCC. ... More
AuthorsKumamoto M, Nishiwaki T, Matsuo N, Kimura H, Matsushima K
JournalEur Respir J
PubMed ID19324956
'Clinical use of bone marrow mesenchymal stem cells (BMMSCs) holds great promise for regenerative medicine in intractable lung diseases, such as lung fibrosis or acute respiratory distress syndrome. However, a severe obstacle to the clinical application of BMMSC transplantation is the time-consuming, laborious processes required for cell culture. In ... More
Gene expression profiling defines ATP as a key regulator of human dendritic cell functions.
AuthorsBles N, Horckmans M, Lefort A, Libert F, Macours P, El Housni H, Marteau F, Boeynaems JM, Communi D
JournalJ Immunol
PubMed ID17785789
'Extracellular ATP and PGE2 are two cAMP-elevating agents inducing semimaturation of human monocyte-derived dendritic cells (MoDCs). We have extensively compared the gene expression profiles induced by adenosine 5''-O-(3-thiotriphosphate) (ATPgammaS) and PGE2 in human MoDCs using microarray technology. At 6 h of stimulation, ATPgammaS initiated an impressive expression profile compared ... More
The effects of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high-light treatment in cold conditions.
AuthorsRapacz M, Wolanin B, Hura K, Tyrka M
JournalAnn Bot
PubMed ID18245808
'BACKGROUND AND AIMS: Cold acclimation modifies the balance of the energy absorbed and metabolized in the dark processes of photosynthesis, which may affect the expression of cold-regulated (COR) genes. At the same time, a gradual acclimation to the relatively high light conditions is observed, thereby minimizing the potential for photo-oxidative ... More
The cytotoxicity of vitamin E is both vitamer- and cell-specific and involves a selectable trait.
AuthorsMcCormick CC, Parker RS
JournalJ Nutr
PubMed ID15570034
'During a study of the effect of vitamin E in activated mouse macrophages, we observed a reduction in the viability of cells treated with various forms of vitamin E. We show in this report that some tocopherols (both gamma- and delta-tocopherol) are cytotoxic to some but not all cell types. ... More
Differential expression of microRNA-675, microRNA-139-3p and microRNA-335 in benign and malignant adrenocortical tumours.
'Background For the clinical management of adrenocortical neoplasms it is crucial to correctly distinguish between benign and malignant tumours. Even histomorphologically based scoring systems do not allow precise separation in single lesions, thus novel parameters are desired which offer a more accurate differentiation. The tremendous potential of microRNAs (miRNAs) as ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
AuthorsChen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
Journal
PubMed ID
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Mapping accessible chromatin regions using Sono-Seq.
AuthorsAuerbach RK, Euskirchen G, Rozowsky J, Lamarre-Vincent N, Moqtaderi Z, Lefrancois P, Struhl K, Gerstein M, Snyder M
JournalProc Natl Acad Sci U S A
PubMed ID19706456
'Disruptions in local chromatin structure often indicate features of biological interest such as regulatory regions. We find that sonication of cross-linked chromatin, when combined with a size-selection step and massively parallel short-read sequencing, can be used as a method (Sono-Seq) to map locations of high chromatin accessibility in promoter regions. ... More
Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo.
AuthorsLienenklaus S, Cornitescu M, Zietara N, Lyszkiewicz M, Gekara N, Jablonska J, Edenhofer F, Rajewsky K, Bruder D, Hafner M, Staeheli P, Weiss S
JournalJ Immunol
PubMed ID19667093
'Type I IFN is a major player in innate and adaptive immune responses. Besides, it is involved in organogenesis and tumor development. Generally, IFN responses are amplified by an autocrine loop with IFN-beta as the priming cytokine. However, due to the lack of sensitive detection systems, where and how ... More
A gain-of-function mutation of Arabidopsis cryptochrome1 promotes flowering.
AuthorsExner V, Alexandre C, Rosenfeldt G, Alfarano P, Nater M, Caflisch A, Gruissem W, Batschauer A, Hennig L
JournalPlant Physiol
PubMed ID20926618
'Plants use different classes of photoreceptors to collect information about their light environment. Cryptochromes are blue light photoreceptors that control deetiolation, entrain the circadian clock, and are involved in flowering time control. Here, we describe the cry1-L407F allele of Arabidopsis (Arabidopsis thaliana), which encodes a hypersensitive cryptochrome1 (cry1) protein. Plants ... More
Hypoglycemia from IGF2 overexpression associated with activation of fetal promoters and loss of imprinting in a metastatic hemangiopericytoma.
AuthorsLawson EA, Zhang X, Crocker JT, Wang WL, Klibanski A
JournalJ Clin Endocrinol Metab
PubMed ID19383775
'CONTEXT: The mechanism of IGF2 overexpression in non-islet-cell tumor hypoglycemia is not understood. OBJECTIVE: We investigated the imprinting control and promoter usage for IGF2 expression to identify a mechanism for increased IGF-II production in non-islet-cell tumor hypoglycemia. PATIENT AND METHODS: A patient with metastatic hemangiopericytoma was studied. Tissue from the ... More
Epigenetic alterations in RASSF1A in human aberrant crypt foci.
'CpG island methylation (CIM) is an epigenetic mechanism for transcriptional silencing that occurs at various stages of colon tumorigenesis. CIM has been found in serrated adenomas and hyperplastic polyps. There is also evidence for hypermethylation in aberrant crypt foci (ACF) that are found in resected colons from cancer patients. Our ... More
Histone deacetylases 1 and 2 form a developmental switch that controls excitatory synapse maturation and function.
AuthorsAkhtar MW, Raingo J, Nelson ED, Montgomery RL, Olson EN, Kavalali ET, Monteggia LM
JournalJ Neurosci
PubMed ID19553468
'The structural assembly of synapses can be accomplished in a rapid time frame, although most nascent synapses formed during early development are not fully functional and respond poorly to presynaptic action potentials. The mechanisms that are responsible for this delay in synapse maturation are unknown. Histone deacetylases (HDACs) regulate the ... More
A single vegetative actin isovariant overexpressed under the control of multiple regulatory sequences is sufficient for normal Arabidopsis development.
AuthorsKandasamy MK, McKinney EC, Meagher RB
JournalPlant Cell
PubMed ID19304937
'The relative significance of gene regulation and protein isovariant differences remains unexplored for most gene families, particularly those participating in multicellular development. Arabidopsis thaliana encodes three vegetative actins, ACT2, ACT7, and ACT8, in two ancient and highly divergent subclasses. Mutations in any of these differentially expressed actins revealed only ... More
The role of interleukin-6 in lipopolysaccharide-induced fever by mechanisms independent of prostaglandin E2.
AuthorsNilsberth C, Elander L, Hamzic N, Norell M, Lonn J, Engstrom L, Blomqvist A
JournalEndocrinology
PubMed ID19022895
'Fever has been shown to be elicited by prostaglandin E(2) (PGE(2)) binding to its receptors on thermoregulatory neurons in the anterior hypothalamus. The signals that trigger PGE(2) production are thought to include proinflammatory cytokines, such as IL-6. However, although the presence of IL-6 is critical for fever, IL-6 by itself ... More
Host structural carbohydrate induces vector transmission of a bacterial plant pathogen.
AuthorsKilliny N, Almeida RP
JournalProc Natl Acad Sci U S A
PubMed ID20018775
'Many insect-borne pathogens have complex life histories because they must colonize both hosts and vectors for successful dissemination. In addition, the transition from host to vector environments may require changes in gene expression before the pathogen''s departure from the host. Xylella fastidiosa is a xylem-limited plant-pathogenic bacterium transmitted by leafhopper ... More
Bruton's tyrosine kinase separately regulates NFkappaB p65RelA activation and cytokine interleukin (IL)-10/IL-12 production in TLR9-stimulated B Cells.
AuthorsLee KG, Xu S, Wong ET, Tergaonkar V, Lam KP
JournalJ Biol Chem
PubMed ID18276597
'B lymphocytes express both B cell receptor and Toll-like receptors (TLR). We show here that Bruton''s tyrosine kinase (Btk), a critical component in B cell receptor signaling, is also involved in TLR9 signaling in B cells. Stimulation of B cells with TLR9 ligand CpG oligodeoxynucleotide (ODN) leads to transient ... More
IL-23-dependent and -independent enhancement pathways of IL-17A production by lactic acid.
AuthorsYabu M, Shime H, Hara H, Saito T, Matsumoto M, Seya T, Akazawa T, Inoue N
JournalInt Immunol
PubMed ID21131367
'Interleukin-17A (IL-17A) is a cytokine produced by T(h)17 cells that plays an important role in inflammatory and autoimmune diseases and cancer. Stimulation with IL-6, transforming growth factor-beta , IL-21, IL-1beta and IL-23 is required for differentiation of T(h)17 cells and the production of IL-17A. Recently, we reported that tumor-derived lactic ... More
Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export.
AuthorsWoo JL, Berk AJ
JournalJ Virol
PubMed ID17079297
'Theadenovirus type 5 (Ad5) E1B-55K and E4orf6 proteins are required together to stimulate viral late nuclear mRNA export to the cytoplasm and to restrict host cell nuclear mRNA export during the late phase of infection. Previous studies have shown that these two viral proteins interact with the cellular proteins elongins ... More