'Adenosine 5''-triphosphate is a universal molecule in all living cells, where it functions in bioenergetics and cell signaling. To understand how the concentration of ATP is regulated by cell metabolism and in turn how it regulates the activities of enzymes in the cell it would be beneficial if we could ... More
A graphical simulation model of the entire DNA process associated with the analysis of short tandem repeat loci.
AuthorsGill P, Curran J, Elliot K
JournalNucleic Acids Res
PubMed ID15681615
'The use of expert systems to interpret short tandem repeat DNA profiles in forensic, medical and ancient DNA applications is becoming increasingly prevalent as high-throughput analytical systems generate large amounts of data that are time-consuming to process. With special reference to low copy number (LCN) applications, we use a graphical ... More
Measuring nasal bacterial load and its association with otitis media.
AuthorsSmith-Vaughan H, Byun R, Nadkarni M, Jacques NA, Hunter N, Halpin S, Morris PS, Leach AJ
JournalBmc Ear Nose Throat Disord
PubMed ID16686940
'""BACKGROUND: Nasal colonisation with otitis media (OM) pathogens, particularly Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis, is a precursor to the onset of OM. Many children experience asymptomatic nasal carriage of these pathogens whereas others will progress to otitis media with effusion (OME) or suppurative OM. We observed a ... More
Diagnostic role of circulating free plasma DNA detection in patients with localized prostate cancer.
AuthorsAltimari A, Grigioni AD, Benedettini E, Gabusi E, Schiavina R, Martinelli A, Morselli-Labate AM, Martorana G, Grigioni WF, Fiorentino M
JournalAm J Clin Pathol
PubMed ID18426736
"To analyze the potential diagnostic relevance of free plasma DNA (FPDNA), we enrolled 64 patients with localized prostate cancer (CaP). FPDNA was quantified by real-time polymerase chain reaction assessment of the HTERT gene in blood samples from 64 patients with CaP and 45 healthy males. Methylation of the GSTP1 ... More
Estimating the integrity of aged DNA samples by CE
A CE/UV method was developed to separate by a micellar system the four DNA bases and other five purinic-pyrimidinic compounds (5-methyl-cytosine, uracil, xanthyne, hypoxanthyne and 5-bromo-uracil). Selectivity, precision, accuracy and sensitivity were assessed and proved to be suitable for the analysis of the primary structure of DNA. This method was ... More
In silico and in vitro comparative analysis to select, validate and test SNPs for human identification.
AuthorsGiardina E, Pietrangeli I, Martone C, Asili P, Predazzi I, Marsala P, Gabriele L, Pipolo C, Ricci O, Solla G, Sineo L, Spinella A, Novelli G
JournalBmc Genomics
PubMed ID18076761
BACKGROUND: The recent advances in human genetics have recently provided new insights into phenotypic variation and genome variability. Current forensic DNA techniques involve the search for genetic similarities and differences between biological samples. Consequently the selection of ideal genomic biomarkers for human identification is crucial in order to ensure the ... More
The orphan nuclear receptor estrogen receptor-related receptor gamma negatively regulates BMP2-induced osteoblast differentiation and bone formation.
AuthorsJeong BC, Lee YS, Park YY, Bae IH, Kim DK, Koo SH, Choi HR, Kim SH, Franceschi RT, Koh JT, Choi HS
JournalJ Biol Chem
PubMed ID19324883
Estrogen receptor-related receptor gamma (ERRgamma/ERR3/NR3B3) is a member of the orphan nuclear receptor with important functions in development and homeostasis. Recently it has been reported that ERRalpha is involved in osteoblast differentiation and bone formation. In the present study we examined the role of ERRgamma in osteoblast differentiation. Here, we ... More
Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
AuthorsPalmer JC, Baig S, Kehoe PG, Love S
JournalAm J Pathol
PubMed ID19541930
Alzheimer's disease (AD) is thought to be caused by the accumulation of amyloid beta (Abeta) peptide within the brain. Endothelin-converting enzyme-2 (ECE-2), which is expressed in neural tissues, cleaves 'big endothelin' to produce the vasoconstrictor endothelin-1. ECE-2 also degrades Abeta. We have examined ECE-2 expression in the temporal cortex of ... More
Fluorescence energy transfer-labeled primers 4 for high-performance forensic DNA profiling
AuthorsYeung, SHI; Seo, TS; Crouse, CA; Greenspoon, SA; Chiesl, TN; Ban, JD; Mathies, RA
Journal
PubMed ID
A fluorescence energy transfer (ET) dye-labeled STR typing system (ET 16-plex) is developed for the markers used in the commercial STR typing kit PowerPlex 16, and its performance assessed using a 96-lane microfabricated capillary array electrophoresis (μCAE) system. The ET 16-plex amplicons displayed 1.6-9-fold higher fluorescence intensities compared to those ... More
Complete nucleotide sequence analysis of plasmids in strains of Staphylococcus aureus clone USA300 reveals a high level of identity among isolates with closely related core genome sequences.
A community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strain known as pulsed-field type USA300 (USA300) is epidemic in the United States. Previous comparative whole-genome sequencing studies demonstrated that there has been recent clonal emergence of a subset of USA300 isolates, which comprise the epidemic clone. Although the core genomes of these isolates ... More
Technical Note: Efficiency of Total Demineralization and Ion-Exchange Column for DNA Extraction from Bone
We investigated whether a combination of recently introduced methods, total demineralization and ion-exchange columns, would increase DNA recovery from old bone. Ten bone samples taken after a burial period of ∼60 years were used in this study. Bone powder was digested using total or incomplete demineralization. DNA was extracted by ... More