SYBR™ Green Universal 预混液
SYBR™ Green Universal 预混液
引用和文献 (1)
Applied Biosystems™

SYBR™ Green Universal 预混液

以便捷的单管形式包含了基于 SYBR™ Green 染料的 –PCR 扩增和检测所需的一切成分。Applied Biosystems™ SYBR™ Green了解更多信息
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货号数量
43643465 x 5 mL
43444631 x 1 mL
43091551 x 5 mL
43643442 x 5 mL
431270410 x 5 mL
43349731 x 50 mL
货号 4364346
价格（CNY)
17,715.00
Each
添加至购物车
数量:
5 x 5 mL
价格（CNY)
17,715.00
Each
添加至购物车
以便捷的单管形式包含了基于 SYBR™ Green 染料的 –PCR 扩增和检测所需的一切成分。Applied Biosystems™ SYBR™ Green PCR 预混液将 SYBR™ Green I 染料、AmpliTaq Gold™ DNA 聚合酶、加入 dUTP 的 dNTP、惰性参比1和优化的缓冲液都组合在便捷的单管中。

•在 2–8°C 下储存的预混组分可大幅缩短测定试剂配制时间
• SYBR™ Green I 染料可检测双链 DNA，因此无需使用特异性探针
• AmpliTaq Gold™ DNA 聚合酶可较大限度避免形成非特异性产物，以获得卓越的性能
• dUTP 与尿嘧啶-DNA 糖基化酶联用可大幅降低残留污染
• 专利缓冲液可增强性能和可靠性

极大限度提高灵活性和便捷性
Applied Biosystems™ SYBR™ Green PCR 预混液不再需要靶标特异性的 TaqMan™ 探针，可以更低的成本实现尽可能高的灵活性。SYBR™ Green I 染料是一种双链 DNA 结合染料，可检测 PCR 过程中产生的任何双链 DNA。热启动酶 AmpliTaq Gold™ DNA 聚合酶可尽量减少非特异性产物形成（包括引物二聚体），从而获得卓越的性能和灵敏度。提供被动内参1，可用于对非 PCR 相关荧光波动的标准化。这样可尽量减少各种原因（例如移液误差或样品蒸发）导致的孔间差异。SYBR™ Green I 染料是靶标鉴定（筛选测定）或需要有限数量的测定时的理想选择。

替代产品
试用 PowerUp SYBR Green 预混液，这是我们新推出的高性能、基于 SYBR 染料的预混液，以极具竞争力的价格提供出色的性能。借助 PowerUp SYBR Green 预混液，我们已经能够充分发挥 SYBR Green PCR 预混液的优势，并为您的基因表达分析增加了更多功能。
仅供科研使用。不可用于诊断程序。
规格
适用于（设备）7000 系统、7300 系统、7500 系统、7700 系统、7900HT 系统、Applied Biosystems StepOnePlus™ Fast 实时荧光定量 PCR 系统、QuantStudio™、StepOne™（标准模式）、StepOnePlus™（标准模式）、ViiA™ 7 系统
产品规格管装
反应次数1000 次反应
参比荧光染料ROX（预混）
聚合酶AmpliTaq Gold DNA 聚合酶
产品线SYBR
产品类型实时荧光定量 PCR SYBR 预混液
数量5 x 5 mL
样品类型DNA（基因组）、cDNA
足够用于1000次 50 μL 反应
容量5 x 5 mL
最大浓度2X
检测方法SYBR
适用于（应用）基因表达
高 GC PCR 扩增效果高
标签或染料SYBR Green
PCR 方法qPCR
反应速度标准
Unit SizeEach
内容与储存
5 x 5 mL 样品瓶中含有 SYBR Green 1 染料、AmpliTaq Gold™ DNA 聚合酶、含 dUTP 的 dNTP、惰性参比染料 1 (ROX) 和经优化的缓冲液成分的 2X 混合物。试剂足够进行1000次反应（基于 50 μl 的反应体积）。

在 -20°C 下储存。

保证的有效期至少为60天（确切有效期印于产品和检验报告书上）。

常见问题解答 (FAQ)

Can I use SYBR Green PCR Master Mix (Cat. No. 4309155, 4364344, 4364346, 4312704, 4334973) in Fast mode?

SYBR Green PCR Master Mix is not supported for use with Fast Mode thermal cycling conditions. When using SYBR Green PCR Master Mix on the StepOne, 7500 Fast, or 7900HT Fast instruments, use Standard mode thermal cycling conditions.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

When using SYBR Green dye chemistry on an Applied Biosystems 7900HT Fast, a 7500 or 7500 Fast, or a 7300 Real-Time PCR System, is there an option to set the quencher to reflect the fact that there is no TaqMan probe being used in the reaction?

Yes. On the Detector Manager for the System, set the Quencher to “Non Fluorescent”.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Is the use of AmpErase UNG necessary for performing reverse transcription reactions?

No. We do not recommend that you use AmpErase UNG when performing reverse transcription. When using a dNTP mix with dUTP in a RT reaction, uracil will get incorporated into the cDNA generated from your RNA template. AmpErase UNG (uracil N-glycosylase), through an enzymatic reaction, will cleave single or double stranded DNA dUTP containing sequences.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Can Power SYBR Green PCR Master Mix (Cat. No. 4368577, 4367659, 4368706, 4368702, 4368708, 4367660) be substituted directly for the SYBR Green PCR Master Mix (Cat. No. 4309155, 4364344, 4364346, 4312704, 4334973)?

The Power SYBR Green PCR Master Mix is a new and improved formulation of the regular SYBR Green PCR Master Mix. You may need to verify your assays in order to start using the Power SYBR Green PCR Master Mix. For more information on verifying your reaction with the Power SYBR Green PCR Master Mix, please refer to the Power SYBR Green PCR Master Mix protocol.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the difference in sensitivity between TaqMan chemistry vs. SYBR Green reagent chemistry?

Sensitivity can actually be equivalent when using TaqMan chemistry and SYBR Green reagent chemistry. It might seem that a TaqMan assay with fluorescent signal generated by a sequence-specific probe would always be more sensitive than a SYBR Green reagent assay, but a poorly designed TaqMan assay could theoretically be less specific than a well-designed SYBR Green reagent assay. However, the potential for detection of primer dimers and non-specific products using SYBR Green chemistry is more likely to result in loss of sensitivity when attempting to quantitate lower copy numbers.

For more information on Real-Time PCR chemistries, please refer to the following Application Notes, which you can find on our website through Technical Resources, or by entering the titles in the main Search field: “Real-Time PCR Vs. Traditional PCR”, “Essentials of Real Time PCR”, and “Selection of Reagents for Real-Time PCR”.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

View all SYBR™ Green PCR 预混液 FAQs

引用和文献 (1)

引用和文献
Abstract
Pharmacological ER stress promotes hepatic lipogenesis and lipid droplet formation.
Authors:Lee JS, Mendez R, Heng HH, Yang ZQ, Zhang K,
Journal:Am J Transl Res
PubMed ID:22347525
Endoplasmic Reticulum (ER) stress refers to a condition of accumulation of unfolded or misfolded proteins in the ER lumen. A variety of biochemical stimuli or pathophysiologic conditions can directly or indirectly induce ER stress, leading to activation of an ER-originated adaptive signaling response called Unfolded Protein Response (UPR). Recent studies ... More