7500 Fast Real-Time PCR System, tower, QST - FAQs

What run conditions should I use for the TaqMan® Advanced miRNA assays with the discontinued 7900HT Real-Time PCR System?

We recommend using the same thermal profile as listed for the 7500 and 7500 Fast systems (Table 8) in the TaqMan® Advanced miRNA Assays User Guide: https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2F100027897_TaqManAdv_miRNA_Assays_UG.pdf