替代产品:试用 TaqMan Fast Advanced 预混液,这是我们较高性能的探针预混液。借助 TaqMan Fast Advanced 预混液,我们已经能够充分发挥 TaqMan Fast Universal PCR 预混液的优势,并为您的基因表达分析增加了更多功能。
仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)2 X
适用于(设备)7500 Fast 系统、7900HT Fast 系统、StepOne™、Fast 模式、StepOnePlus™、Fast 模式
产品规格管装
反应次数1250 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq DNA 聚合酶
产品线AmpErase、TaqMan
产品类型Fast 通用型 PCR 预混液
数量1,250 reactions
样品类型DNA(基因组)、cDNA
足够用于250 次反应
容量1.25 mL
最大浓度2X
检测方法引物-探针
适用于(应用)基因表达, 基因分型, miRNA 分析
高 GC PCR 扩增效果高
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
此5盒装产品内含五盒试剂盒,每盒包含2管 2X TaqMan™ Fast 通用型 PCR 预混液(每管 1.25 ml)。
在 2°C 至 8°C 下储存。
保证的有效期至少为60天(确切有效期印于产品和检验报告书上)。
常见问题解答 (FAQ)
Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?
The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.
引用和文献 (43)
引用和文献
Abstract
Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro.
Authors:Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K, Saito S, Shimotohno KW, Suzuki T, Shimizu Y, Tobita R, Hijikata M, Takaku H, Shimotohno K
Journal:J Virol
PubMed ID:19369330
Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5' untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, ... More
Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I
Authors:Lisik, W; Tejpal, N; Gong, YQ; Skelton, TS; Ganachari, M; Bremer, EG; Kloc, M; Ghobrial, RM
Journal:
PubMed ID:
'Background: The allochimeric MHC class I molecule [α1h1/u]-RT1. Aa that contains donor-type (Wistar Furth, WF; RT1u) epitopes displayed on recipient-type (ACI, RT1a) administered in conjunction with sub-therapeutic dose of cyclosporine (CsA) induces indefinite survival of heterotopic cardiac allografts in rat model. In vascularized transplantation models, the spleen contributes to graft ... More
Activation of Macrophages by Polysaccharide-protein Complex from Lycium barbarum L.
Authors:Chen, ZS; Soo, MY; Srinivasan, N; Tan, BKH; Chan, SH
Journal:
PubMed ID:
'Macrophages play crucial roles in innate immunity. This paper reports that a polysaccharide-protein complex isolated from Lycium barbarum (LBP) is able to activate macrophages. LBP was isolated from Lycium barbarum fruit and separated to five homogenous fractions, designated LBPF1, LBPF2, LBPF3, LBPF4 and LBPF5. It was found that LBP (50 ... More
Fusion of Epstein-Barr virus nuclear antigen-1-derived glycine-alanine repeat to trans-dominant HIV-1 Gag increases inhibitory activities and survival of transduced cells in vivo
'Trans-dominant human immunodeficiency virus type 1 (HIV-1) Gag derivatives have been shown to efficiently inhibit late steps of HIV-1 replication in vitro by interfering with Gag precursor assembly, thus ranking among the interesting candidates for gene therapy approaches. However, efficient antiviral activities of corresponding transgenes are likely to be counteracted ... More
Prevalence of Chlamydophila psittaci in wild birds-potential risk for domestic poultry, pet birds, and public health?
Authors:Zweifel, D; Hoop, R; Sachse, K; Pospischil, A; Borel, N
Journal:
PubMed ID:
'To determine the prevalence of Chlamydophila psittaci in wild birds, cloacal swabs from 527 songbirds, 442 waterfowl, 84 feral pigeons, and 38 cormorants were examined by Chlamydiaceae-specific real-time polymerase chain reaction (PCR) and ArrayTube microarray assays for chlamydial species determination and genotyping of C. psittaci. Inconclusive cases were further characterized ... More