TaqMan™ MicroRNA 逆转录试剂盒
TaqMan™ MicroRNA 逆转录试剂盒
Applied Biosystems™

TaqMan™ MicroRNA 逆转录试剂盒

Applied Biosystems™ TaqMan™ MicroRNA 逆转录试剂盒可提供 TaqMan™ MicroRNA 检测试剂盒实现卓越性能所需的1000个反应组分。该试剂盒的组分与 TaqMan™了解更多信息
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货号足够用于
43665971000 次反应
4366596200 次反应
货号 4366597
价格（CNY)
13,074.00
Each
添加至购物车
足够用于:
1000 次反应
价格（CNY)
13,074.00
Each
添加至购物车
Applied Biosystems™ TaqMan™ MicroRNA 逆转录试剂盒可提供 TaqMan™ MicroRNA 检测试剂盒实现卓越性能所需的1000个反应组分。该试剂盒的组分与 TaqMan™ MicroRNA 检测试剂盒提供的 RT 引物共同用于将 miRNA 转换成 cDNA。

高特异性试剂盒，仅用于定量成熟 miRNA，排除前体 miRNA。一种保存有限样品的灵敏试剂盒，只需要 1-10 毫微克总 RNA。快速、简单、可放大，两步定量 RT-PCR 分析法可在3小时内产生高质量结果。
仅供科研使用。不可用于诊断程序。
规格
保真度（相对于 Taq）10 X
最终产品类型cDNA
反应次数1000 次反应
最佳反应温度42°C
产品线TaqMan™
产品类型MicroRNA 逆转录试剂盒
数量1,000 reactions
反应形式分离组分
逆转录酶MultiScribe™
样品类型RNA
运输条件干冰
尺寸（最终产品）200 bp 或更小
足够用于1000 次反应
最大浓度10X
检测方法引物-探针
适用于（应用）miRNA 分析
高 GC PCR 扩增效果高
PCR 方法两步法 RT-qPCR
反应速度快速
Unit SizeEach
内容与储存
含有能够进行1000个反应的 10X RT 缓冲液、dNTP 混合物 w/dTTp（共 100 mM）、RNase 抑制剂 (20 U/µL) 和 MultiScribe™ RT 酶 (50 U/µL)。储存于 -15 到 -25°C。

常见问题解答 (FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Is it possible to combine several miRNA RT primers together for reverse transcription, and if so, how?

Yes. If you are using individual TaqMan MicroRNA Assays, you can prepare your own RT and PreAmp pools. Refer to Protocol for Creating Custom RT and Preamplification Pools using TaqMan MicroRNA Assays (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4465407_CustomRT-preamp_UG.pdf) for detailed information. Although this protocol has been tested by our development group, we recommend that you validate the performance of the particular pool that you are interested in working with. At a minimum, we recommend running a No Template Control (NTC). The NTC is crucial to identify any primer interactions that may increase the background.

Why does the TaqMan MicroRNA Reverse Transcription Kit (4366597) protocol have an initial 16 degree C step before the reverse transcription step at 42 degrees C?

The 30 min incubation at 16°C allows the RT primer to anneal to miRNA template properly. See the complete protocol at this link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What concentration of cDNA or range do we recommend for miRNA assays when using the TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)?

1-10 ng of total RNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all TaqMan™ MicroRNA 逆转录试剂盒 FAQs