采用 Applied Biosystems™ Power SYBR™ Green 预混液获得出色的核酸定量。Power SYBR™ 预混液提供出色的灵敏度和重现性,能在较宽的模板浓度范围内检测出低至两个拷贝数的靶基因了解更多信息
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货号
数量
4367659
1 x 5 mL
4368577
1 x 1 mL
4368706
2 x 5 mL
4368702
5 x 5 mL
4368708
10 x 5 mL
4367660
1 x 50 mL
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货号 4367659
价格(CNY)
4,545.00
Each
添加至购物车
数量:
1 x 5 mL
价格(CNY)
4,545.00
Each
添加至购物车
采用 Applied Biosystems™ Power SYBR™ Green 预混液获得出色的核酸定量。Power SYBR™ 预混液提供出色的灵敏度和重现性,能在较宽的模板浓度范围内检测出低至两个拷贝数的靶基因。
• 以便捷的单管形式包含了基于 SYBR™ Green 染料的 –PCR 扩增和检测所需的一切成分。Power SYBR™ Green PCR 预混液包含除模板和引物外的全部组分,可用于基于 SYBR™ Green 试剂的卓越实时 PCR 分析 • 其优化的配方含有高纯度的 AmpliTaq Gold™ DNA 聚合酶 LD,可实现比经典 SYBR™ Green PCR 预混液更高的灵敏度 • Power SYBR™ Green PCR 预混液含有兼容 AmpErase™ UNG 的 dTTP/dUTP 混合物,以尽量减少遗留污染 • 包括专用版本的 ROX™ 染料,它是一种内部被动参比,可以对非 PCR–相关的荧光波动进行标准化,以尽量减少由于各种原因(如移液错误和样品蒸发)而产生的孔间差异 • 基于 PowerSYBR™Green 试剂的 PCR 化学试剂产品可以轻松替代 Applied Biosystems™ 方案中现有的 SYBR™ Green PCR 预混液,且构建和热循环条件完全相同
替代产品 试用 PowerUp SYBR Green 预混液,这是我们新推出的高性能、基于 SYBR 染料的预混液,以极具竞争力的价格提供出色的性能。借助 PowerUp SYBR Green 预混液,我们已经能够充分发挥 Power SYBR Green PCR 预混液的优势,并为您的基因表达分析增加了更多功能。
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.