Power SYBR™ Green PCR 预混液
<i>Power</i> SYBR&trade; Green PCR 预混液
Applied Biosystems™

Power SYBR™ Green PCR 预混液

采用 Applied Biosystems™ Power SYBR™ Green 预混液获得出色的核酸定量。Power SYBR™ 预混液提供出色的灵敏度和重现性,能在较宽的模板浓度范围内检测出低至两个拷贝数的靶基因了解更多信息
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货号数量
43676591 x 5 mL
43685771 x 1 mL
43687062 x 5 mL
43687025 x 5 mL
436870810 x 5 mL
43676601 x 50 mL
货号 4367659
价格(CNY)
4,545.00
Each
添加至购物车
数量:
1 x 5 mL
价格(CNY)
4,545.00
Each
添加至购物车
采用 Applied Biosystems™ Power SYBR™ Green 预混液获得出色的核酸定量。Power SYBR™ 预混液提供出色的灵敏度和重现性,能在较宽的模板浓度范围内检测出低至两个拷贝数的靶基因。

• 以便捷的单管形式包含了基于 SYBR™ Green 染料的 –PCR 扩增和检测所需的一切成分。Power SYBR™ Green PCR 预混液包含除模板和引物外的全部组分,可用于基于 SYBR™ Green 试剂的卓越实时 PCR 分析
• 其优化的配方含有高纯度的 AmpliTaq Gold™ DNA 聚合酶 LD,可实现比经典 SYBR™ Green PCR 预混液更高的灵敏度
Power SYBR™ Green PCR 预混液含有兼容 AmpErase™ UNG 的 dTTP/dUTP 混合物,以尽量减少遗留污染
• 包括专用版本的 ROX™ 染料,它是一种内部被动参比,可以对非 PCR–相关的荧光波动进行标准化,以尽量减少由于各种原因(如移液错误和样品蒸发)而产生的孔间差异
• 基于 PowerSYBR™Green 试剂的 PCR 化学试剂产品可以轻松替代 Applied Biosystems™ 方案中现有的 SYBR™ Green PCR 预混液,且构建和热循环条件完全相同

替代产品
试用 PowerUp SYBR Green 预混液,这是我们新推出的高性能、基于 SYBR 染料的预混液,以极具竞争力的价格提供出色的性能。借助 PowerUp SYBR Green 预混液,我们已经能够充分发挥 Power SYBR Green PCR 预混液的优势,并为您的基因表达分析增加了更多功能。
仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7300 系统、7500 系统、7700 系统、7900HT Fast 系统、Applied Biosystems StepOnePlus™ Fast 实时荧光定量 PCR 系统、QuantStudio™ 12k Flex、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOne™(标准模式)、StepOnePlus™(标准模式)、ViiA™ 7 系统
产品规格管装
反应次数200 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶、AmpliTaq Gold LD
产品线SYBR™
产品类型实时荧光定量 PCR SYBR 预混液
纯度或质量等级LD(低 DNA 含量)
数量1 x 5 mL
样品类型DNA(基因组)、cDNA
运输条件湿冰
足够用于200次反应,每次 50 μL
容量1 x 5 mL
最大浓度2X
检测方法SYBR
适用于(应用)基因表达
高 GC PCR 扩增效果
标签或染料SYBR Green
PCR 方法qPCR
反应速度标准
Unit SizeEach
内容与储存
2X 混合物含有 SYBR™ Green 1 染料、AmpliTaq Gold DNA 聚合酶 LD、含 dUTP/dTTP 混合物的 dNTP、惰性参比染料 1 和经优化的缓冲液组分。含有 1 × 5 mL,足以进行200次 50 µl 反应。

在 -20°C 下储存。

保证的有效期至少为60天(确切有效期印于产品和检验报告书上)。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.