TaqMan™ 基因表达预混液
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TaqMan™ 基因表达预混液
Applied Biosystems™

TaqMan™ 基因表达预混液

利用 TaqMan 基因表达预混液在九个对数的线性动态范围内获得精确可靠的实时 qPCR 定量。TaqMan 基因表达预混液是经过优化的2X 混合液,包含除模板和引物外的全部组分,用于实现低至一个靶标拷贝的灵敏检测。延长的室温稳定性为自动化液体处理系统提供一致结果,使之成为隔夜或跨周末高通量样品处理的选择TaqMan 基因表达预混液已通过了解更多信息
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货号数量
43700481 x 1 mL
436954210 x 5 mL
43695142 x 5 mL
43695105 x 5 mL
43690161 x 5 mL
43700741 x 50 mL
货号 4370048
价格(CNY)
1,382.00
Each
添加至购物车
数量:
1 x 1 mL
价格(CNY)
1,382.00
Each
添加至购物车
利用 TaqMan 基因表达预混液在九个对数的线性动态范围内获得精确可靠的实时 qPCR 定量。TaqMan 基因表达预混液是经过优化的2X 混合液,包含除模板和引物外的全部组分,用于实现低至一个靶标拷贝的灵敏检测。延长的室温稳定性为自动化液体处理系统提供一致结果,使之成为隔夜或跨周末高通量样品处理的选择TaqMan 基因表达预混液已通过 TaqMan 基因表达测定和 Applied Biosystems 实时系统进行验证,为各种基于基因表达的实时荧光定量 qPCR 应用提供精确定量,包括:
• 罕见转录物检测
• 基因表达分析
• 病原体检测和病毒载量量化
• 两个靶标的共扩增
• 基因家族成员的特异性检测

TaqMan 基因表达预混液包含 AmpliTaq Gold DNA 聚合酶 UP(超纯)(用于热启动活化和改进细菌靶标检测)、dNTP 与 dTTP/dUTP 和尿嘧啶-DNA 糖基化酶 (UDG) 的混合物(用于尽可能地减少残留的 PCR 污染)以及基于专有 ROX 染料的被动内参(用于在 Applied Biosystems 实时荧光定量 PCR 仪器上获得极高精度)。

替代产品:试用 TaqMan Fast Advanced 预混液,这是我们较高性能的探针预混液。借助 TaqMan Fast Advanced 预混液,我们已经能够充分发挥 TaqMan 基因表达预混液的优势,并为您的基因表达分析增加了更多功能。

仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7000系统,7300系统,7500系统,7900 HT 系统,Applied Biosystems StepOnePlus™ Fast 实时荧光定量 PCR 系统 StepOne™,标准模式,StepOnePlus™,标准模式
反应次数40 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶
产品线TaqMan™
纯度或质量等级UP (Ultra Pure)
数量1 x 1 mL
样品类型DNA(基因组)、cDNA
容量1 x 1 mL
检测方法引物-探针
适用于(应用)基因表达
形式液体
PCR 方法qPCR
反应速度标准
Unit SizeEach
内容与储存
1管 (1 mL) 2X TaqMan™ 基因表达预混液,足以进行40次反应。在 2°C 至 8°C 下储存。


TaqMan™ 基因表达预混液含有 AmpliTaq Gold™ DNA 聚合酶(超纯)、尿嘧啶-DNA 糖基化酶、dNTP(含 dUTP)、ROX™ 惰性参比染料和经优化的缓冲液成分。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.