High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 1000 reactions - FAQs

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can I order the RNase Inhibitor from the High-Capacity cDNA Reverse Transcription Kit separately?

Yes. You can order the RNase Inhibitor separately as Cat. No. N8080119.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

• High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
• High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.