Cancer exosomes trigger fibroblast to myofibroblast differentiation.
AuthorsWebber J, Steadman R, Mason MD, Tabi Z, Clayton A
JournalCancer Res
PubMed ID21098712
There is a growing interest in the cell-cell communication roles in cancer mediated by secreted vesicles termed exosomes. In this study, we examined whether exosomes produced by cancer cells could transmit information to normal stromal fibroblasts and trigger a cellular response. We found that some cancer-derived exosomes could trigger elevated ... More
Blastema induction in aneurogenic state and Prrx-1 regulation by MMPs and FGFs in Ambystoma mexicanum limb regeneration.
AuthorsSatoh A,makanae A,Hirata A,Satou Y
JournalDevelopmental biology
PubMed ID21539833
Deregulated LAP2α expression in cervical cancer associates with aberrant E2F and p53 activities.
AuthorsWard MC,van der Watt PJ,Tzoneva G,Leaner VD
JournalIUBMB life
PubMed ID21990273
Resistance to a novel antichlamydial compound is mediated through mutations in Chlamydia trachomatis secY.
A novel and quantitative high-throughput screening approach was explored as a tool for the identification of novel compounds that inhibit chlamydial growth in mammalian cells. The assay is based on accumulation of a fluorescent marker by intracellular chlamydiae. Its utility was demonstrated by screening 42,000 chemically defined compounds against Chlamydia ... More
Sex specific changes in placental growth and MAPK following short term maternal dexamethasone exposure in the mouse.
AuthorsCuffe JS,Dickinson H,Simmons DG,Moritz KM
JournalPlacenta
PubMed ID21974799
Interferon lambda inhibits dengue virus replication in epithelial cells.
AuthorsPalma-Ocampo HK,Flores-Alonso JC,Vallejo-Ruiz V,Reyes-Leyva J,Flores-Mendoza L,Herrera-Camacho I,Rosas-Murrieta NH,Santos-López G
JournalVirology journal
PubMed ID26411318
BACKGROUND: In viral disease, infection is controlled at the cellular level by type I interferon (IFN-I), but dengue virus (DENV) has the ability to inhibit this response. Type III interferon, also known as lambda IFN (IFN-III or IFN-λ), is a complementary pathway to the antiviral response by IFN-I. This work ... More
Oral gene application using chitosan-DNA nanoparticles induces transferable tolerance.
AuthorsGoldmann K,Ensminger SM,Spriewald BM
JournalClinical and vaccine immunology : CVI
PubMed ID22933401
Oral tolerance is a promising approach to induce unresponsiveness to various antigens. The development of tolerogenic vaccines could be exploited in modulating the immune response in autoimmune disease and allograft rejection. In this study, we investigated a nonviral gene transfer strategy for inducing oral tolerance via antigen-encoding chitosan-DNA nanoparticles (NP). ... More
Increased microRNA-221/222 and decreased estrogen receptor α in the cervical portion of the uterosacral ligaments from women with pelvic organ prolapse.
AuthorsShi Z,Zhang T,Zhang L,Zhao J,Gong J,Zhao C
JournalInternational urogynecology journal
PubMed ID22527538
Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2.
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID22802639
Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, the in vitro directed differentiation of human pluripotent stem cells into mature hepatocytes remains challenging. Little attention has so far been paid to variations among hiPSC lines in terms of their hepatic ... More
Cytokine expression in response to root canal infection in gnotobiotic mice.
AuthorsMaciel KF,Neves de Brito LC,Tavares WL,Moreira G,Nicoli JR,Vieira LQ,Ribeiro Sobrinho AP
JournalInternational endodontic journal
PubMed ID22233143
Anti-senescence effects of DNA methyltransferase inhibitor RG108 in human bone marrow mesenchymal stromal cells.
AuthorsOh YS,Jeong SG,Cho GW
JournalBiotechnology and applied biochemistry
PubMed ID25952632
XPC deficiency is related to APE1 and OGG1 expression and function.
BACKGROUND: Sarcopenia is defined as the ageârelated loss of skeletal muscle mass and function. While all humans lose muscle with age, 2â5% of elderly adults develop functional consequences (disabilities). The aim of this study was to investigate muscle myogenesis in healthy elderly adults, with or without sarcopenia, compared with middleâaged ... More
Heat stress induces ferroptosis-like cell death in plants.
In plants, regulated cell death (RCD) plays critical roles during development and is essential for plant-specific responses to abiotic and biotic stresses. Ferroptosis is an iron-dependent, oxidative, nonapoptotic form of cell death recently described in animal cells. In animal cells, this process can be triggered by depletion of glutathione (GSH) ... More
Circulating fibrocyte mobilization in negative pressure wound therapy.
AuthorsChen D,Zhao Y,Li Z,Shou K,Zheng X,Li P,Qi B,Yu A
JournalJournal of cellular and molecular medicine
PubMed ID28211211
Nonâhealing diabetic wounds are difficult to treat. They also create heavy financial burdens for both patients and society. Negative pressure wound therapy (NPWT) has been adopted to treat intractable wounds and has proved to be effective. However, the mechanisms that underlie the effects of this treatment are not entirely understood. ... More
Tryptanthrin reduces mast cell proliferation promoted by TSLP through modulation of MDM2 and p53.
Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization.
AuthorsWu TH,Kuo YY,Lee HH,Kuo JC,Ou MH,Chang ZF
JournalScientific reports
PubMed ID27350000
It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a significant ... More
Regulation of Schwann cell proliferation and migration by miR-1 targeting brain-derived neurotrophic factor after peripheral nerve injury.
AuthorsYi S,Yuan Y,Chen Q,Wang X,Gong L,Liu J,Gu X,Li S
JournalScientific reports
PubMed ID27381812
Peripheral nerve injury is a global problem that causes disability and severe socioeconomic burden. Brain-derived neurotrophic factor (BDNF) benefits peripheral nerve regeneration and becomes a promising therapeutic molecule. In the current study, we found that microRNA-1 (miR-1) directly targeted BDNF by binding to its 3â²-UTR and caused both mRNA degradation ... More
miR-769-5p suppressed cell proliferation, migration and invasion by targeting TGFBR1 in non-small cell lung carcinoma.
MicroRNAs (miRNAs) are key regulators of multiple cancers, including non-small cell lung carcinoma (NSCLC). The aim of this study was to determine the expression pattern of miR-769-5p in NSCLC and to investigate its biological role during tumorigenesis. We showed that miR-769-5p was significantly downregulated and predicted poor prognosis in NSCLC ... More
Euryops arabicus Promotes Healing of Excised Wounds in Rat Skin: Emphasis on Its Collagen-Enhancing, Antioxidant, and Anti-Inflammatory Activities.
AuthorsAbdel-Lateff A,Abdel-Naim AB,Alarif WM,Algandaby MM,Alburae NA,Alghamdi AM,Nasrullah MZ,Fahmy UA
JournalOxidative medicine and cellular longevity
PubMed ID33574987
Euryops arabicus Steud (E. arabicus) belongs to the family Asteraceae. It has several uses in folk medicine in the Arabian Peninsula. The current study aimed at evaluating the wound healing properties of the E. arabicus extract in rats. Primarily, E. arabicus successfully accelerated cell migration in vitro and it also ... More
Impact of dietary ω3 polyunsaturated fatty acid supplementation on brown and brite adipocyte function.
The recent characterization of functional brown adipose tissue in adult humans has opened new perspectives for regulation of energy expenditure with respect to obesity and diabetes. Furthermore, dietary recommendations have taken into account the insufficient dietary intake of Ï3 PUFAs and the concomitant excessive intake of Ï6 PUFA associated with ... More
The Effect of Testosterone Replacement on Intramedullary, Inguinal and Visceral Fat in Ovariectomized Rats.
JournalRevista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia
PubMed ID32107765
ObjectiveâThe present article aims to evaluate the impact of testosterone treatment on the expansion of visceral, subcutaneous and intramedullary adipose tissue of ovariectomized rats and the visceral and subcutaneous fat expression of peroxisome proliferator-activated receptors (PPARs) gamma. MethodsâIn total 48 female Wistar rats were castrated and randomly divided into 6 ... More
Lack of association between viral load and severity of acute bronchiolitis in infants.
JournalJornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia
PubMed ID27832233
OBJECTIVE: To investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. METHODS: This was a two-year, cross-sectional study of infants ⤠12 months of age with bronchiolitis at the time of admission to a tertiary hospital. For the identification of respiratory ... More
The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids.
AuthorsLee JS,Ryu HR,Cha JY,Baik HS
JournalJournal of microbiology (Seoul, Korea)
PubMed ID26428924
Hypoxia increases the heterogeneity of melanoma cell populations and affects the response to vemurafenib.
AuthorsPucciarelli D,Lengger N,Takáčová M,Csaderova L,Bartosova M,Breiteneder H,Pastorekova S,Hafner C
JournalMolecular medicine reports
PubMed ID26936534
The MAZ transcription factor is a downstream target of the oncoprotein Cyr61/CCN1 and promotes pancreatic cancer cell invasion via CRAF-ERK signaling.
Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC). However, the mechanism of action of MAZ in PDAC progression is largely unknown. Here, we present evidence that MAZ mRNA ... More
Overexpression of ATPase Na+/+ transporting alpha 1 polypeptide, ATP1A1, correlates with clinical diagnosis and progression of esophageal squamous cell carcinoma.
This study aims to identify new upregulated genes related to secretory or membranous proteins to help detect esophageal squamous cell carcinoma (ESCC). First, we performed microarray-based screening of esophageal tumors from both N-nitrosomethylbenzylamine- and arecoline-induced F344 rats and seventeen human ESCC specimens. Candidate genes were validated by quantitative PCR (qPCR) ... More
CYP2E1 regulates the development of radiation-induced pulmonary fibrosis via ER stress- and ROS-dependent mechanisms.
AuthorsSon B,Kwon T,Lee S,Han I,Kim W,Youn H,Youn B
JournalAmerican journal of physiology. Lung cellular and molecular physiology
PubMed ID28798253
Deletion at the GCNT2 Locus Causes Autosomal Recessive Congenital Cataracts.
PURPOSE: The aim of this study is to identify the molecular basis of autosomal recessive congenital cataracts (arCC) in a large consanguineous pedigree. METHODS: All participating individuals underwent a detailed ophthalmic examination. Each patientâs medical history, particularly of cataracts and other ocular abnormalities, was compiled from available medical records and ... More
Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies.
Rottlerin is a natural polyphenolic compound that was initially indicated as a PKCδ inhibitor. However, it was recently revealed that it may target a number of molecules and have biological effects on various cell types and is considered as a possible agent for tumor and cell proliferative diseases. Psoriasis is ... More
3,5,7,3',4'-Pentamethoxyflavone Enhances the Barrier Function through Transcriptional Regulation of the Tight Junction in Human Intestinal Caco-2 Cells.
Molecular Typing of Mastadenoviruses in Simultaneously Collected Nasopharyngeal Swabs and Stool Samples from Children Hospitalized for Acute Bronchiolitis, Acute Gastroenteritis, and Febrile Seizures.
This study determines and compares the frequency of human mastadenovirus (HAdV) presence in children with acute bronchiolitis (AB), acute gastroenteritis (AGE), and febrile seizures (FS), ascertains types of HAdVs associated with each individual syndrome and contrasts the findings with a control group of children. The presence of HAdVs was ascertained ... More
Orosomucoid-like 3 Supports Rhinovirus Replication in Human Epithelial Cells.
JournalAmerican journal of respiratory cell and molecular biology
PubMed ID32078788
Polymorphism at the 17q21 gene locus and wheezing responses to rhinovirus (RV) early in childhood conspire to increase the risk of developing asthma. However, the mechanisms mediating this geneâenvironment interaction remain unclear. In this study, we investigated the impact of one of the 17q21-encoded genes, ORMDL3 (orosomucoid-like 3), on RV ... More
Physicochemical, Pre-Clinical, and Biological Evaluation of Viscosity Optimized Sodium Iodide-Incorporated Paste.
This study aimed to investigate the impact of different viscosities of silicone oil on the physicochemical, pre-clinical usability, and biological properties of a sodium iodide paste. Six different paste groups were created by mixing therapeutic molecules, sodium iodide (D30) and iodoform (I30), with calcium hydroxide and one of the three ... More
Hepcidin inhibits Smad3 phosphorylation in hepatic stellate cells by impeding ferroportin-mediated regulation of Akt.
Hepatic stellate cell (HSC) activation on liver injury facilitates fibrosis. Hepatokines affecting HSCs are largely unknown. Here we show that hepcidin inhibits HSC activation and ameliorates liver fibrosis. We observe that hepcidin levels are inversely correlated with exacerbation of fibrosis in patients, and also confirm the relationship in animal models. ... More
Macauba (Acrocomia aculeata) Pulp Oil Prevents Adipogenesis, Inflammation and Oxidative Stress in Mice Fed a High-Fat Diet.
AuthorsSant' Ana CT,Agrizzi Verediano T,Grancieri M,Toledo RCL,Tako E,Costa NMB,Martino HSD,de Barros FAR
JournalNutrients
PubMed ID36904250
Macauba is a palm tree native to Brazil, which fruits are rich in oil. Macauba pulp oil has high contents of oleic acid, carotenoids, and tocopherol, but its effect on health is unknown. We hypothesized that macauba pulp oil would prevent adipogenesis and inflammation in mice. Thus, the purpose of ... More
Inducible Major Vault Protein Plays a Pivotal Role in Double-Stranded RNA- or Virus-Induced Proinflammatory Response.
AuthorsPeng N,Liu S,Xia Z,Ren S,Feng J,Jing M,Gao X,Wiemer EA,Zhu Y
JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID26843330
A Three-Dimensional Chondrocyte-Macrophage Coculture System to Probe Inflammation in Experimental Osteoarthritis.
AuthorsSamavedi S,Diaz-Rodriguez P,Erndt-Marino JD,Hahn MS
JournalTissue engineering. Part A
PubMed ID27736317
The goal of the present study was to develop a fully three-dimensional (3D) coculture system that would allow for systematic evaluation of the interplay between activated macrophages (AMs) and chondrocytes in osteoarthritic disease progression and treatment. Toward this end, our coculture system was first validated against existing in vitro osteoarthritis ... More
Down-Regulation of MicroRNA-184 Is Associated With Corneal Neovascularization.
BACKGROUND: Decades of cytotoxic and more recently immunotherapy treatments for malignant glioma have had limited success due to dynamic intra-tumoral heterogeneity. The dynamic interplay of cancer cell subpopulations has been found to be under the control of proteins in the cancer microenvironment. EGF-containing fibulin-like extracellular matrix protein (EFEMP1) (also fibulin-3) ... More
Development and validation of an RBP gene signature for prognosis prediction in colorectal cancer based on WGCNA.
BACKGROUND: RNA binding proteins (RBPs) have been implicated in oncogenesis and progression in various cancers. However, the potential value of RBPs as prognostic indicators and therapeutic targets in colorectal cancer (CRC) requires further investigation. METHODS: Four thousand eighty two RBPs were collected from literature. The weighted gene co-expression network analysis ... More
Ependymal polarity defects coupled with disorganized ciliary beating drive abnormal cerebrospinal fluid flow and spine curvature in zebrafish.
Idiopathic scoliosis (IS) is the most common spinal deformity diagnosed in childhood or early adolescence, while the underlying pathogenesis of this serious condition remains largely unknown. Here, we report zebrafish ccdc57 mutants exhibiting scoliosis during late development, similar to that observed in human adolescent idiopathic scoliosis (AIS). Zebrafish ccdc57 mutants ... More
Stomatal properties of Arabidopsis cauline and rice flag leaves and their contributions to seed production and grain yield.
AuthorsDing M,Zhu Y,Kinoshita T
JournalJournal of experimental botany
PubMed ID36520996
Cauline leaves on the inflorescence stem of Arabidopsis thaliana may play important roles in supplying photosynthetic products to sinks, such as floral organs. Flag leaves in rice (Oryza sativa) have a higher photosynthetic capacity than other leaves, and are crucial for increasing grain yield. However, the detailed properties of stomata ... More
The Monascus metabolite monascin against TNF-α-induced insulin resistance via suppressing PPAR-γ phosphorylation in C2C12 myotubes.
AuthorsLee BH,Hsu WH,Liao TH,Pan TM
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
PubMed ID21777645
Identification of active denitrifiers in rice paddy soil by DNA- and RNA-based analyses.
AuthorsYoshida M,Ishii S,Fujii D,Otsuka S,Senoo K
JournalMicrobes and environments
PubMed ID22972387
Denitrification occurs markedly in rice paddy fields; however, few microbes that are actively involved in denitrification in these environments have been identified. In this study, we used a laboratory soil microcosm system in which denitrification activity was enhanced. DNA and RNA were extracted from soil at six time points after ... More
Cytokine analysis in lesions refractory to endodontic treatment.
AuthorsHenriques LC,de Brito LC,Tavares WL,Vieira LQ,Ribeiro Sobrinho AP
JournalJournal of endodontics
PubMed ID22099900
Chronic stress targets posttranscriptional mechanisms to rapidly upregulate α1C-subunit of Cav1.2b calcium channels in colonic smooth muscle cells.
AuthorsLi Q,Sarna SK
JournalAmerican journal of physiology. Gastrointestinal and liver physiology
PubMed ID21051529
Chronic stress elevates plasma norepinephrine, which enhances expression of the α(1C)-subunit of Ca(v)1.2b channels in colonic smooth muscle cells within 1 h. Transcriptional upregulation usually does not explain such rapid protein synthesis. We investigated whether chronic stress-induced release of norepinephrine utilizes posttranscriptional mechanisms to enhance the α(1C)-subunit. We performed experiments ... More
Cytomegalovirus CC chemokine promotes immune cell migration.
Cytomegaloviruses manipulate the host chemokine/receptor axis by altering cellular chemokine expression and by encoding multiple chemokines and chemokine receptors. Similar to human cytomegalovirus (HCMV), rat cytomegalovirus (RCMV) encodes multiple CC chemokine-analogous proteins, including r129 (HCMV UL128 homologue) and r131 (HCMV UL130 and MCMV m129/130 homologues). Although these proteins play a ... More
Stimulation of the hypothalamic ventromedial nuclei by pituitary adenylate cyclase-activating polypeptide induces hypophagia and thermogenesis.
AuthorsResch JM,Boisvert JP,Hourigan AE,Mueller CR,Yi SS,Choi S
JournalAmerican journal of physiology. Regulatory, integrative and comparative physiology
PubMed ID21957159
Numerous studies have demonstrated that the hypothalamic ventromedial nuclei (VMN) regulate energy homeostasis by integrating and utilizing behavioral and metabolic mechanisms. The VMN heavily express pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptors (PAC1R). Despite the receptor distribution, most PACAP experiments investigating affects on feeding have focused on intracerebroventricular administration ... More
Yakammaoto inhibits enterovirus 71 infection by reducing viral attachment, internalization, replication, and translation.
PURPOSE. To identify the changes in postnatal mouse conjunctival forniceal gene expression and their regulation by Klf4 during the eye-opening stage when the goblet cells first appear. METHODS. Laser microdissection (LMD) was used to collect conjunctival forniceal cells from postnatal (PN) day 9, PN14 and PN20 wild-type (WT), and PN14 ... More
Individual transcriptional activity of estrogen receptors in primary breast cancer and its clinical significance.
To predict the efficacy of hormonal therapy at the individual-level, immunohistochemical methods are used to analyze expression of classical molecular biomarkers such as estrogen receptor (ER), progesterone receptor (PgR), and HER2. However, the current diagnostic standard is not perfect for the individualization of diverse cases. Therefore, establishment of more accurate ... More
A Genetic Variation in the Y Chromosome Among Modern Japanese Males Related to Several Physiological and Psychological Characteristics.
AuthorsMatsunaga M,Ohtsubo Y,Masuda T,Noguchi Y,Yamasue H,Ishii K
JournalFrontiers in behavioral neuroscience
PubMed ID34924970
Previous studies in population genetics have proposed that the Y-chromosomal (Y-DNA) haplogroup D ancestor likely originated from Africa. The haplogroup D branch next started Out-of-Africa migration, rapidly expanded across Eurasia, and later diversified in East Asia. Y-DNA haplogroup D-M55, one of the branches of haplogroup D, is only found in ... More
Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC.
AuthorsKimura S,Naganuma S,Susuki D,Hirono Y,Yamaguchi A,Fujieda S,Sano K,Itoh H
JournalOncology reports
PubMed ID20428818
Wnt-5a-regulated miR-101b controls COX2 expression in hippocampal neurons.
AuthorsCodocedo JF,Inestrosa NC
JournalBiological research
PubMed ID26895946
BACKGROUND: Wnt-5a is a member of the WNT family of secreted lipoglycoproteins, whose expression increases during development; moreover, Wnt-5a plays a key role in synaptic structure and function in the adult nervous system. However, the mechanism underlying these effects is still elusive. MicroRNAs (miRNAs) are a family of small non-coding ... More
Inhibitory effects of T/L-type calcium channel blockers on tubulointerstitial fibrosis in obstructed kidneys in rats.
Interactions between RNA binding proteins (RBPs) and genes are not well understood, especially in regulation of angiogenesis. The RBP HuR binds to the AU-rich (ARE) regions of labile mRNAs, facilitating their translation into protein and has been hypothesized to be a tumor-maintenance gene. Elevated levels of cytoplasmic HuR directly correlate ... More
Suppressing memory T cell activation induces islet allograft tolerance in alloantigen-primed mice.
JournalTransplant international : official journal of the European Society for Organ Transplantation
PubMed ID20536791
Early-life nicotine or cotinine exposure produces long-lasting sleep alterations and downregulation of hippocampal corticosteroid receptors in adult mice.
Early-life exposure to environmental toxins like tobacco can permanently re-program body structure and function. Here, we investigated the long-term effects on mouse adult sleep phenotype exerted by early-life exposure to nicotine or to its principal metabolite, cotinine. Moreover, we investigated whether these effects occurred together with a reprogramming of the ... More
Effect of enhanced expression of connexin 43 on sunitinib-induced cytotoxicity in mesothelioma cells.
AuthorsUzu M,Sato H,Yamada R,Kashiba T,Shibata Y,Yamaura K,Ueno K
JournalJournal of pharmacological sciences
PubMed ID26003083
Evaluation of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in infected tomato plants.
AuthorsMascia T,Santovito E,Gallitelli D,Cillo F
JournalMolecular plant pathology
PubMed ID21029324
The quantification of messenger RNA expression levels by realâtime reverseâtranscription polymerase chain reaction requires the availability of reference genes that are stably expressed regardless of the experimental conditions under study. We examined the expression variations of a set of eight candidate reference genes in tomato leaf and root tissues subjected ... More
Transient receptor potential canonical 3 (TRPC3) mediates thrombin-induced astrocyte activation and upregulates its own expression in cortical astrocytes.
JournalThe Journal of neuroscience : the official journal of the Society for Neuroscience
PubMed ID20881130
Reactive astrogliosis, defined by abnormal morphology and excessive cell proliferation, is a characteristic response of astrocytes to CNS injuries, including intracerebral hemorrhage. Thrombin, a major blood-derived serine protease, leaks into the brain parenchyma upon bloodâbrain barrier disruption and can induce brain injury and astrogliosis. Transient receptor potential canonical (TRPC) channels, ... More
Pachymic acid stimulates glucose uptake through enhanced GLUT4 expression and translocation.
Extracellular cystine influences human preadipocyte differentiation and correlates with fat mass in healthy adults.
AuthorsElkafrawy H,Mehanna R,Ali F,Barghash A,Dessouky I,Jernerén F,Turner C,Refsum H,Elshorbagy A
JournalAmino acids
PubMed ID34519922
Plasma cysteine is associated with human obesity, but it is unknown whether this is mediated by reduced, disulfide (cystine and mixed-disulfides) or protein-bound (bCys) fractions. We investigated which cysteine fractions are associated with adiposity in vivo and if a relevant fraction influences human adipogenesis in vitro. In the current study, ... More
8-Hydroxydaidzein Downregulates JAK/STAT, MMP, Oxidative Phosphorylation, and PI3K/AKT Pathways in K562 Cells.
AuthorsWu PS,Wang CY,Chen PS,Hung JH,Yen JH,Wu MJ
JournalBiomedicines
PubMed ID34944720
A metabolite isolated from fermented soybean, 8-hydroxydaidzein (8-OHD, 7,8,4â²-trihydroxyisoflavone, NSC-678112), is widely used in ethnopharmacological research due to its anti-proliferative and anti-inflammatory effects. We reported previously that 8-OHD provoked reactive oxygen species (ROS) overproduction, and induced autophagy, apoptosis, breakpoint cluster region-Abelson murine leukemia viral oncogene (BCR-ABL) degradation, and differentiation in ... More
Analysis of gut microbiota and intestinal integrity markers of inpatients with major depressive disorder.
JournalProgress in neuro-psychopharmacology & biological psychiatry
PubMed ID32827611
Staphylococcus aureus AI-2 quorum sensing associates with the KdpDE two-component system to regulate capsular polysaccharide synthesis and virulence.
AuthorsZhao L,Xue T,Shang F,Sun H,Sun B
JournalInfection and immunity
PubMed ID20498265
Autoinducer 2 (AI-2) is widely recognized as a signal molecule for intra- and interspecies communication in Gram-negative bacteria, but its signaling function in Gram-positive bacteria, especially in Staphylococcus aureus, remains obscure. Here we reveal the role of LuxS in the regulation of capsular polysaccharide synthesis in S. aureus NCTC8325 and ... More
Development of PCR assays to detect iridovirus infections among captive and wild populations of Missouri River sturgeon.
AuthorsKurobe T,Kwak KT,MacConnell E,McDowell TS,Mardones FO,Hedrick RP
JournalDiseases of aquatic organisms
PubMed ID21290894
Anti-CD44 monoclonal antibody inhibits heart transplant rejection mediated by alloantigen-primed CD4(+) memory T cells in nude mice.
AuthorsWang F,Chen J,Shao W,Xie B,Wang Y,Lan T,Thorlacius H,Qi Z
JournalImmunological investigations
PubMed ID20718664
Spatiotemporal nuclear factor interleukin-6 expression in the rat brain during lipopolysaccharide-induced fever is linked to sustained hypothalamic inflammatory target gene induction.
AuthorsDamm J,Luheshi GN,Gerstberger R,Roth J,Rummel C
JournalThe Journal of comparative neurology
PubMed ID21192080
Glyceraldehyde-3-phosphate dehydrogenase present in extracellular vesicles from Leishmania major suppresses host TNF-alpha expression.
AuthorsDas P,Mukherjee A,Adak S
JournalThe Journal of biological chemistry
PubMed ID34534548
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) fulfills various physiological roles that are unrelated to its glycolytic function. However, to date, the nonglycolytic function of GAPDH in trypanosomal parasites is absent from the literature. Exosomes secreted from Leishmania, like entire parasites, were found to have a significant impact on macrophage cell signaling and function, ... More
Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers.
AuthorsImafuku K,Kamaguchi M,Natsuga K,Nakamura H,Shimizu H,Iwata H
JournalCell and tissue research
PubMed ID33635425
Weighted Gene Co-Expression Network Analysis Identifies ANGPTL4 as a Key Regulator in Diabetic Cardiomyopathy via FAK/SIRT3/ROS Pathway in Cardiomyocyte.
BACKGROUND: Diabetic cardiomyopathy (DbCM) is characterized by initial impairment of left ventricular relaxation followed by contractile dysfunction. Despite intensive research, the exact mechanism remains so far unsolved. METHODS: We constructed weighted gene co-expression network analysis (WGCNA) to screen gene modules that were closely related with DbCM based on the GSE5606 ... More
Stanniocalcin-1 regulates re-epithelialization in human keratinocytes.
AuthorsYeung BH,Wong CK
JournalPloS one
PubMed ID22069492
Stanniocalcin-1 (STC1), a glycoprotein hormone, is believed to be involved in various biological processes such as inflammation, oxidative responses and cell migration. Riding on these emerging evidences, we hypothesized that STC1 may participate in the re-epithelialization during wound healing. Re-epithelialization is a critical step that involves keratinocyte lamellipodia (e-lam) formation, ... More
Improving Generation of Cardiac Organoids from Human Pluripotent Stem Cells Using the Aurora Kinase Inhibitor ZM447439.
AuthorsLee SJ,Kim HA,Kim SJ,Lee HA
JournalBiomedicines
PubMed ID34944767
Drug-induced cardiotoxicity reduces the success rates of drug development. Thus, the limitations of current evaluation methods must be addressed. Human cardiac organoids (hCOs) derived from induced pluripotent stem cells (hiPSCs) are useful as an advanced drug-testing model; they demonstrate similar electrophysiological functionality and drug reactivity as the heart. How-ever, similar ... More
GPRC5B promotes collagen production in myofibroblasts.
AuthorsTakizawa N,Hironaka T,Mae K,Ueno T,Horii Y,Nagasaka A,Nakaya M
JournalBiochemical and biophysical research communications
PubMed ID34023784
Respiratory syncytial virus induces phosphorylation of mTOR at ser2448 in CD8 T cells from nasal washes of infected infants.
Respiratory syncytial virus (RSV)âspecific CD8(+) T cell responses do not protect against reinfection. Activation of mammalian target of rapamycin (mTOR) impairs memory CD8(+) T cell differentiation. Our hypothesis was that RSV inhibits the formation of CD8(+) T cells memory responses through mTOR activation. To explore this, human and mouse T ... More
Phylogeny of Plant CAMTAs and Role of AtCAMTAs in Nonhost Resistance to Xanthomonas oryzae pv. oryzae.
Calmodulin-binding transcription activator (CAMTA) constitutes one of the most important Ca(2+)/CaM-regulated transcription factor families in plants. Nevertheless, the phylogeny, protein interaction network, and role in nonhost resistance of plant CAMTAs are not well understood. In this study, 200 CAMTA genes were identified from 35 species representing four major plant lineages. ... More
Toxic effects of ZnSe/ZnS quantum dots on the reproduction and genotoxiticy of rare minnow (Gobiocypris rarus).
AuthorsDing Y,Yang Y,Chen J,Chen H,Wu Y,Jin L
JournalComparative biochemistry and physiology. Toxicology & pharmacology : CBP
PubMed ID33915279
Sphingosine kinase 1 is upregulated with lysophosphatidic acid receptor 2 in human colorectal cancer.
AuthorsShida D,Inoue S,Yoshida Y,Kodaka A,Tsuji T,Tsuiji M
JournalWorld journal of gastroenterology
PubMed ID26937138
AIM: To examine the expression of SphK1, an oncogenic kinase that produces sphingosine 1-phosphate (S1P), and its correlation with the expression of LPAR2, a major lysophosphatidic acid (LPA) receptor overexpressed in various cancers, in human colorectal cancer. METHODS: Real-time reverse-transcription polymerase chain reaction was used to measure the mRNA expression ... More
Orientation and expression of methicillin-resistant Staphylococcus aureus small RNAs by direct multiplexed measurements using the nCounter of NanoString technology.
AuthorsBeaume M,Hernandez D,Docquier M,Delucinge-Vivier C,Descombes P,François P
JournalJournal of microbiological methods
PubMed ID21195730
Effect of endothelial progenitor cell-derived extracellular vesicles on endothelial cell ferroptosis and atherosclerotic vascular endothelial injury.
AuthorsLi L,Wang H,Zhang J,Chen X,Zhang Z,Li Q
JournalCell death discovery
PubMed ID34493702
Atherosclerosis (AS) is a chronic inflammatory disorder characterized by endothelial dysfunction. Endothelial progenitor cells (EPCs) can overcome endothelial dysfunction and reduce AS risk. This study focused on the role of EPC-secreted extracellular vesicles (EPC-EVs) in AS. First, mouse EPCs and mouse aortic endothelial cells (MAECs) were isolated and identified. EVs ... More
Solanum nigrum Protects against Hepatic Fibrosis via Suppression of Hyperglycemia in High-Fat/Ethanol Diet-Induced Rats.
AuthorsTai CJ,Choong CY,Shi YC,Lin YC,Wang CW,Lee BH,Tai CJ
JournalMolecules (Basel, Switzerland)
PubMed ID26927042
Background: Advanced glycation end products (AGEs) signal through the receptor for AGE (RAGE), which can lead to hepatic fibrosis in hyperglycemia and hyperlipidemia. We investigated the inhibitory effect of aqueous extracts from Solanum nigrum (AESN) on AGEs-induced RAGE signaling and activation of hepatic stellate cells (HSCs) and hyperglycemia induced by ... More
Inositol requiring mutant 80 (INO80) is a chromatin remodeler that regulates pluripotency maintenance of embryonic stem cells and reprogramming of somatic cells into pluripotent stem cells. However, the roles and mechanisms of INO80 in porcine pre-implantation embryonic development remain largely unknown. Here, we show that INO80 modulates trophectoderm epithelium permeability ... More
Single-cell transcriptome atlas of human mesenchymal stem cells exploring cellular heterogeneity.
BACKGROUND: The heterogeneity of mesenchymal stem cells (MSCs) is poorly understood, thus limiting clinical application and basic research reproducibility. Advanced singleâcell RNA sequencing (scRNAâseq) is a robust tool used to analyse for dissecting cellular heterogeneity. However, the comprehensive singleâcell atlas for human MSCs has not been achieved. METHODS: This study ... More
A demethylating agent enhances chemosensitivity to vinblastine in a xenograft model of renal cell carcinoma.
miR-145 transgenic mice develop cardiopulmonary complications leading to postnatal death.
AuthorsThomas S,Manivannan S,Sawant D,Kodigepalli KM,Garg V,Conway SJ,Lilly B
JournalPhysiological reports
PubMed ID34523259
BACKGROUND: Both downregulation and elevation of microRNA miRâ145Â has been linked to an array of cardiopulmonary phenotypes, and a host of studies suggest that it is an important contributor in governing the differentiation of cardiac and vascular smooth muscle cell types. METHODS AND RESULTS: To better understand the role of elevated ... More
ALL blasts drive primary mesenchymal stromal cells to increase asparagine availability during asparaginase treatment.
Mechanisms underlying the resistance of acute lymphoblastic leukemia (ALL) blasts to l-asparaginase are still incompletely known. Here we demonstrate that human primary bone marrow mesenchymal stromal cells (MSCs) successfully adapt to l-asparaginase and markedly protect leukemic blasts from the enzyme-dependent cytotoxicity through an amino acid trade-off. ALL blasts synthesize and ... More
PTP4A3 Is a Prognostic Biomarker Correlated With Immune Infiltrates in Papillary Renal Cell Carcinoma.
AuthorsSong Q,Zheng Y,Wu J,Wang S,Meng L,Yao Q,Li Z,Lian S
JournalFrontiers in immunology
PubMed ID34630392
PTP4A3 plays an important role in the tumorigenesis and metastasis of multiple tumors, but its prognostic role in renal cancer is not well understood. We utilized the Oncomine and Tumor Immunoassay Resource databases to examine the differential expression of PTP4A3 in tumor tissues and normal tissues in breast, urinary tract, ... More
In vitro enhanced differentiation of neural networks in ES gut-like organ from mouse ES cells by a 5-HT4-receptor activation.
AuthorsTakaki M,Misawa H,Matsuyoshi H,Kawahara I,Goto K,Zhang GX,Obata K,Kuniyasu H
JournalBiochemical and biophysical research communications
PubMed ID21333625
Cloning, expression and characterization of arcelin and its impact on digestive enzymes of the stored product insect pest, Callosobruchus maculatus (F.).
AuthorsHilda K,Bhuvaragavan S,Kamatchi R,Meenakumari M,Janarthanan S
JournalPesticide biochemistry and physiology
PubMed ID34955175
Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells.
β-catenin is the key component of the canonical Wnt pathway and plays a crucial role in a multitude of developmental and homeostatic processes. The different tasks of β-catenin are orchestrated by its subcellular localization and participation in multiprotein complexes. To gain a better understanding of β-catenin's role in living cells ... More
Comparative de novo transcriptome analysis identifies salinity stress responsive genes and metabolic pathways in sugarcane and its wild relative Erianthus arundinaceus [Retzius] Jeswiet.
Erianthus arundinaceus [Retzius] Jeswiet, a wild relative of sugarcane has a high biomass production potential and a reservoir of many genes for superior agronomic traits and tolerance to biotic and abiotic stresses. A comparative physiological, anatomical and root transcriptome analysis were carried out to identify the salt-responsive genes and metabolic pathways associated ... More
Quantitative analysis of plant polyamines including thermospermine during growth and salinity stress.
AuthorsNaka Y,Watanabe K,Sagor GH,Niitsu M,Pillai MA,Kusano T,Takahashi Y
JournalPlant physiology and biochemistry : PPB
PubMed ID20137962
Inhibition of TGF-β signaling enables human corneal endothelial cell expansion in vitro for use in regenerative medicine.
AuthorsOkumura N,Kay EP,Nakahara M,Hamuro J,Kinoshita S,Koizumi N
JournalPloS one
PubMed ID23451286
Corneal endothelial dysfunctions occurring in patients with Fuchs' endothelial corneal dystrophy, pseudoexfoliation syndrome, corneal endotheliitis, and surgically induced corneal endothelial damage cause blindness due to the loss of endothelial function that maintains corneal transparency. Transplantation of cultivated corneal endothelial cells (CECs) has been researched to repair endothelial dysfunction in animal ... More
PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.
AuthorsWu Z,Liu D,Yue N,Song H,Luo J,Zhang Z
JournalInternational journal of molecular sciences
PubMed ID34638794
Environmental acclimation ability plays a key role in plant growth, although the mechanism remains unclear. Here, we determined the involvement of Arabidopsis thaliana PLANT DEFENSIN 1 gene AtPDF1.5 in the adaptation to low nitrogen (LN) levels and cadmium (Cd) stress. Histochemical analysis revealed that AtPDF1.5 was mainly expressed in the ... More
Effects of omega-3 fatty acids and metformin combination on diabetic cardiomyopathy in rats through autophagic pathway.