我的PCR反应管在实验后变形了。问题在哪里？
热循环仪的热盖经过设计可保证对微孔板施加合适的压力，以获得高效的PCR反应。使用PCR反应管时，热盖对反应管的压力过大可导致反应管变形。为避免出现这种情况，我们建议使用下述托盘/固定装置：
•货号4381850，用于Veriti系统的MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），和Veriti热循环仪、SimpliAmp热循环仪和Proflex PCR系统兼容。
•货号403081，MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），可用于7000系统、2720热循环仪和GeneAmp 9700型PCR系统。
•货号4379983，用于VeriFlex模块的MicroAmp 96孔托盘——这一托盘适用于MicroAmp联管（或MicroAmp带盖反应管，0.1 mL），可用于Veriti热循环仪、Veriti快速热循环仪、SimpliAmp热循环仪和Proflex PCR系统。
为何SimpliAmp热循环仪入门套装包含了两种不同的托盘？
两种托盘适用于不同的反应管：
•适用于Veriti系统的MicroAmp 96孔托盘/固定装置（货号4381850） - 这一托盘适用于0.2mL不带盖单管（货号N8010533, N8011533），0.2 mL 带圆盖的8连管(货号A30589)和0.2 mL无盖连管(Cat 4316567) 。
•用于VeriFlex模块的MicroAmp 96孔托盘(货号4379983)-此托盘与0.1 mL带盖的单管或连管一起使用。
Why are my PCR tubes crushed/deformed after my PCR run?
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
Why does the SimpliAmp Thermal Cycler starter kit contain two different types of trays?
The two different types of trays are for use with different tubes:
-MicroAmp 96-well Tray/Retainer Set for Veriti Systems (Cat. No. 4381850) - this tray is for use with 0.2 mL single tubes without caps (Cat. Nos. N8010533, N8011533), 0.2 mL 8-tube strip with attached dome caps (Cat. No. A30589), and 0.2 mL tube strips without caps (Cat. No. 4316567)
-MicroAmp 96-well Tray for VeriFlex Blocks (Cat. No. 4379983) - this tray is for use with 0.2 mL single tubes with caps (Cat. Nos. N8010540, N8011540)
Can I run strip tubes on the ViiA 7 instrument?
Yes. Make sure to use the appropriate matching parts. For the 96-well standard block, use the standard 96-well plate adapter (Cat. No. 4459845), the retainer tray for (0.2 mL) tubes, and Optical 8-Tube Strips and Caps (Cat. No. 4316567 and 4323032). For the 96-well Fast block, use the Fast 96-well plate adapter (Cat. No. 4462078), the retainer tray (Cat. No. 4379983), and Fast (0.1 mL) Optical 8-tube Caps and Strips (Cat. Nos. 4323032 and 4358293).
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
