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View additional product information for Tempus™ Spin RNA Isolation Kit - FAQs (4380204)
6 product FAQs found
不能，由于该纯化柱的截留分子量较低（大约为200bp），小RNA将会在使用这种纯化方法时丢失。
不兼容，请遵循Tempus系统的RNA分离方法。Tempus收集管与RiboPure试剂盒不兼容。
No, unfortunately, small RNAs will be lost using this purification method due to the low molecular weight cutoff from the column (approximately 200 bp).
No, please follow the RNA isolation methods described with the Tempus system. The collection tubes are not compatible with the RiboPure kit.
Our general recommendations are that once blood has been collected in the Tempus Blood RNA Tubes, you can store the tube at room temperature for up to 5 days or at 4 degrees C for up to 7 days. For long term storage, the tube can be frozen at -80 degrees C for at least 1.5 years.
Yes, it is okay to add an on-column or post-elution DNase treatment.
For the on-column DNase treatment, use 350 µl of the following solution and incubate for 15 mins at room temperature:
2500 units DNAse I (RNAse-free) in 10 mL of solution containing:
10 mM Tris-HCl, pH 8
2.5 mM MgCl2 . 6H2O
0.1 mM CaCl2 . 2H2O
This would be done between the two Wash 2 steps, and it would be a good idea to add a third Wash 2 step to make sure all of the DNase is washed away.