AgPath-ID™ 一步法 RT-PCR 预混液(含说明书)
Product Image
Applied Biosystems™

AgPath-ID™ 一步法 RT-PCR 预混液(含说明书)

AgPath-ID™ 一步法 RT-PCR 试剂适用于使用快速单管 TaqMan™ 实时逆转录 PCR (RT-PCR) 策略对 RNA了解更多信息
Have Questions?
更改视图buttonViewtableView
货号反应次数
4387424M500 次反应
4387391M1000 次反应
AM1005M100 次反应
货号 4387424M
价格(CNY)
13,829.00
Each
添加至购物车
反应次数:
500 次反应
价格(CNY)
13,829.00
Each
添加至购物车

AgPath-ID™ 一步法 RT-PCR 试剂适用于使用快速单管 TaqMan™ 实时逆转录 PCR (RT-PCR) 策略对 RNA 靶标进行灵敏且稳健的扩增。
•建议用于 RNA 病原体扩增
•对 RNA 靶标实现一致、高特异性和高灵敏度的扩增
•经优化适合与您的靶特异性引物和探针配合使用
•含有 ROX,用于定量荧光信号归一化

AgPath-ID™ 一步法 RT-PCR 试剂可用于快速而简单的反应构建。反应混装在单管内,从而尽可能地减少样品处理错误并加快构建时间。混装后,可在大约一小时内获得结果。试剂盒中包括的 25X RT-PCR 酶混合物含有高效的 ArrayScript™ 逆转录酶(一种能够获得高 cDNA 得率的突变体 MMLV RT)以及 AmpliTaq Gold™ 聚合酶(用于特异性靶标扩增的首选热启动 DNA 聚合酶)。2X RT-PCR 缓冲液经过优化,可实现高效、稳健的逆转录和 PCR,包括惰性参比染料 ROX™ 染料,用于定量荧光信号归一化。

仅供科研使用。不可用于诊断程序。
规格
描述适用于所有 AB 平台,但针对 7500 进行了优化。
保真度(相对于 Taq)25 X
适用于(设备)7500 系统
产品规格管装
反应次数500 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶
产品线AgPath-ID、Ambion
产品类型一步法 RT-PCR 试剂
数量500 次反应
逆转录酶ArrayScript™
足够用于500 次反应
容量7 mL
最大浓度25X
检测方法引物-探针
适用于(应用)动物健康, 病原检测
高 GC PCR 扩增效果
PCR 方法一步法 RT-qPCR
反应速度标准
Unit SizeEach
内容与储存
足以进行 500 次反应(每次 25 µL),含有:

• 7 mL 2 x RT-PCR 缓冲液

• 550 µL 25 x RT-PCR 酶混合物

• 25 mL 无核酸酶水。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.