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查看更多产品信息 MagMAX™ Express-96 Deep Well Plates - FAQs (4388476)
45 个常见问题解答
关闭机器，然后检查仪器是否有可见的损伤。确保正在运行的实验程序使用的是正确的磁头。如果机器没有可见的损伤，请重新打开机器。这时，机器将会重置。如果问题又立即重新出现，则将机器关闭，并将磁头轻轻移到它移动路径的中央，然后再次重启机器。如果问题仍然出现，请拨打客服电话，联系重置磁力棒。
一个可能的原因是塑料的磁棒套由于其本身的设计稍有弯曲。这也是为什么磁棒套是成对包装的，以保持它们的形状。您可以手动调整磁棒套主板的弯曲度，并再试一次。
这种情况有时会发生，但是这不会影响产量，因为样本已经从磁珠上被洗脱下来了。
如果在运行期间点击“停止”，则可暂停；如果再次点击开始，则继续运行。如果点击停止两次，则运行完全终止，无法从实验方案的中间部分开始运行。
如果起始材料过于粘稠，磁力棒将不能完全吸取磁珠。可以尝试稀释样本，并检查样本是否使用正确的方法进行匀浆及裂解。
如果没有向磁棒套架插入磁棒套则程序不会开始运行。
将软布或纸巾用温和的去垢剂、肥皂水或酒精润湿，然后擦拭磁力棒。
您可以根据您的实验操作习惯及具体实验需求选择磁力架。使用AM10027的磁力架，磁珠会聚集每孔的一侧，这样更有利于使用移液器从孔的另一侧吸取液体。AM10050的磁极在孔的周围，因此磁珠会在孔的周围聚集成环，更有利于使用自动化液体操作平台进行操作。
很遗憾，没有。
推荐使用深孔板。对于更大体积的样品（可达400 μL），请使用深孔配置。
由磁套进行的试剂混合发生在液面以下，这样可防止使用MagMAX Express仪器时出现气溶胶和溢出。
我们强烈建议保持每孔的液体体积在指定的限值范围内，以免试剂溢出。
加热模块每分钟升温10°C，因此需要6分钟左右。
加热模块位于仪器内部，在实验方案执行过程中会被自动调用。MagMAX Express-96孔板与PCR反应板（带裙边）都能够通过专门设计的，可互换的加热模块进行加热。在实验方案中，可添加任意数量的加热步骤。在实验方案的执行过程中，当进入加热步骤时，反应板会自动移至专用的加热位置。加热步骤结束后，实验方案将自动进入下一步骤。
注：无法加入冷却步骤。
MagMAX Express-96系统不可靠近磁带、计算机磁碟及信用卡等磁性存储系统，这些物品可能会被MagMAX Express-96磁棒的强力磁场所损坏。MagMAX Express-96磁棒头不可靠近PC显示器，否则将可能造成显示器损坏。操控MagMAX Express-96磁棒头时，不可使用金属工具。
警告：本产品含有强力永磁体。佩戴心脏起搏器或金属义肢的人员不宜使用此设备。心脏起搏器或义肢靠近强磁场时，性能可能受到影响或发生损坏。
MagMAX Express-96的磁体使用非常稳定的材料制成。磁体的磁性不会变弱。不过，极端的机械外力或加热可能会对磁体造成损坏。注：务必使MagMAX Express-96的磁棒头远离其他磁棒或其他磁体。磁体相互撞击会引起严重损坏。
可以。您可在同一次运行中使用深孔板和MagMAX Express-96孔板。因此，您可以先采用较大体积的反应板开始处理，之后将纯化样本洗脱到小体积反应板（MagMAX Express-96孔板）中。
The MagMAX Express-96 深孔板 (货号4388476)及标准板(货号4388475)可与仪器一起使用。< / p >
您可在同一次运行中使用深孔板和KingFisher 96孔板。因此，您可以先采用较大体积的反应板开始处理，之后将纯化样本洗脱到小体积反应板（KingFisher 96孔板或MagMAX Express-96孔板）中。
不行，我们强烈推荐您使用MagMAX板或KingFisher板，因为它们是专为配合KingFisher Flex磁棒套同时使用而设计的，以达到最佳效果。其它制造商生产的板子可能与KingFisher Flex加热模块不兼容。它们还可能引起意料之外的问题，例如可能会由于不同的孔容积和板底高度而引起交叉污染。
带96深孔磁棒头的KingFisher Flex仪器和MagMAX Express-96仪器所需的耗材是相同的。列表如下：
- MagMAX Express-96 深孔板 (货号 4388476)
- MagMAX Express-96 标准板(货号4388475)
- MagMAX Express-96深孔磁棒套(货号4388487)
- MicroAmp 透明封口膜（可选）： (货号. 4306311)
对于带24深孔磁棒头的KingFisher Flex仪器，所需耗材列表如下：
- KingFisher Flex 24深孔板：(货号. 95040480)
- KingFisher Flex 24深孔磁棒套： (货号 97002610)
Turn the machine off, and inspect it for any visible damage. Make sure the correct head is being used for the protocol being run. If the machine shows no visible damage, turn it back on. This should reset the machine. If the problem immediately occurs again, turn the machine off and gently move the magnetic head apparatus to the center of its travel path, then turn the machine on again. If the problem persists, the head may need to be realigned by a service call.
A potential cause is that the plastic tip comb has warped slightly due to its design. This is why they are packed in pairs, so that they maintain their shape. Flex the backbone of the tip comb and try again.
This happens sometimes, but it will not affect the yield because the sample has been released from the particles.
There is a pause function if you hit ‘stop' in the middle of a run. If you hit start again, the run continues. If you hit stop twice, the run stops completely. There is no way to begin a run in the middle of a protocol.
If the starting material is too viscous, the magnetic rods will not be able to collect the particles. Dilute the sample and check that the sample has been properly homogenized and lysed.
The protocol will not start without the tip combs inserted into the tip comb holder.
Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution, soap solution, or alcohol.
It is a personal preference as to which magnetic stand to purchase. Cat. No. AM10027 can be easier if using a pipettor for liquid handling, as beads clump to the side, Cat. No. AM10050 if using a robotic liquid handler, as beads clump in a ring.
No, unfortunately not.
Deep well plates are recommended. Use a deep well configuration for larger sample inputs (up to 400 µL).
Mixing with the tip comb is designed to occur below the liquid surface. This prevents aerosols and splashing from occurring when using the MagMAX Express instrument.
We strongly recommend that you keep the specified volumes within the defined limits to avoid spillover of the reagents.
The heating block warms up about 10 degrees C per minute, so it will take about 6 minutes.
The heating block is located inside the instrument and can be used automatically during the protocol. Both MagMAX Express-96 plates and PCR plates (skirted) can be heated using specially designed, interchangeable heating blocks. Any number of heating steps can be added to the protocol. During the protocol, when the protocol enters the heating step, the plate is automatically moved to the dedicated heating position for heating. After the heating step, the protocol continues automatically to the next step.
Note: No cooling steps can be added.
The MagMAX Express-96 system should not be kept in close proximity to magnetic tapes, computer discs, or other magnetic storage systems, such as credit cards, as these can be damaged by the strong magnetic field of the MagMAX Express-96 heads. Do not hold the MagMAX Express-96 heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling MagMAX Express-96 heads.
Warning: This product contains very strong permanent magnets. People wearing a pacemaker or metallic prosthesis should not use this product. A pacemaker or prosthesis may be affected or damaged if it comes in close contact with a strong magnetic field.
The MagMAX Express-96 magnets are made of material that is very stable. The magnetic field will not get weaker. However, extreme mechanical force or heating can cause damage to the magnets.
Note: It is very important to keep the MagMAX Express-96 heads away from each other and other magnets at all times. Clashing of the magnets together causes serious damage to the magnets.
Yes. Both deep well plates and MagMAX Express-96 standard plates can be used during the same run. Therefore, it is possible to start the processing by using larger volumes (in a deep well plate) and elute the purified nucleic acid to a smaller volume (in a MagMAX Express-96 standard plate).
The MagMAX Express-96 deep well plates (Cat. No. 4388476) and standard plates (Cat. No. 4388475) can be used with the instrument.
The MagMAX Express-96 Deep Well Plates have been discontinued. Please contact our Technical Support team at techsupport@thermofisher.com to request a certificate of conformity.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
Yes, the MagMAX Express and KingFisher plates are equivalent. We recommend the KingFisher plates as an alternative to the discontinued MagMAX Express plates.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
The KingFisher-96 DeepWell plates Cat. No. 96040460 (sterile) and Cat. No. 95040450 (non-sterile) can be used on the MagMAX Express-96 as an alternative to the MagMAX Express-96 DeepWell plates (Cat. No. 4388476)
Yes, both the KingFisher Flex 96 KF and 96 Deep-Well heating blocks can be used for the 96 Deep-Well Head, but they are only compatible with certain MagMAX/KingFisher plates.
- The KingFisher Flex 96 KF Heating Block is compatible with both standard plates and Deep-Well plates (Cat. Nos. 97002540, 4388475, 95040460, 4388476).
- The KingFisher Flex Deep-Well Heating Block is only compatible with Deep-Well plates (Cat. Nos. 95040460, 4388476).
Both deep-well plates and KingFisher 96 plates can be used during the same run. Therefore, it is possible to start the processing by using larger volumes (in a deep-well plate) and elute the purified sample to a smaller volume (in a KingFisher 96 or MagMAX 96 standard plate).
No, we strongly recommend that you use the MagMAX plates or KingFisher plates since they were specifically designed to be used with KingFisher Flex tip combs to attain maximal performance. Plates from other manufacturers may not be compatible with the KingFisher Flex heating blocks. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.