Power SYBR™ Green RNA-to-CT 1Step 试剂盒
<i>Power</i> SYBR&trade; Green RNA-to-C<sub>T </sub>&trade;<i>1Step</i> 试剂盒
Applied Biosystems™

Power SYBR™ Green RNA-to-CT 1Step 试剂盒

通过简单易用的一步法 qRT-PCR 反应获得高灵敏度和特异性的结果。Power SYBR™ Green RNA-to-CT™ 一步法试剂盒的制备方式可确保较大的灵敏度和可靠性。这种新型制备方式可减少引物二聚体引起的假阳性结果,显著提高数据准确性,并能可靠地检测低丰度靶标。其中包括:•AmpliTaq™了解更多信息
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货号反应次数
4389986500 次反应
4391178100 次反应
货号 4389986
价格(CNY)
9,665.00
Each
添加至购物车
反应次数:
500 次反应
价格(CNY)
9,665.00
Each
添加至购物车
通过简单易用的一步法 qRT-PCR 反应获得高灵敏度和特异性的结果。Power SYBR™ Green RNA-to-CT一步法试剂盒的制备方式可确保较大的灵敏度和可靠性。这种新型制备方式可减少引物二聚体引起的假阳性结果,显著提高数据准确性,并能可靠地检测低丰度靶标。其中包括:
•AmpliTaq™ Gold DNA Polymerase UP 用于热启动 qPCR,可较大限度地减少非特异性产物形成
•ArrayScript™ UP 逆转录酶是一种经过工程设计的 RT,可以产生高产量的全长 cDNA
•一种可以减少引物二聚体形成,进而减少可产生假阳性信号的非特异性产物的添加剂
•RNase 抑制剂,防止 RNA 模板降解
•专用 ROX™ 染料是一种被动内参染料,可以用于使非 PCR 相关的荧光波动标准化,从而提高 Applied Biosystems™ 实时荧光定量 PCR 仪器的精密度。
仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7500 Fast 系统、7500 系统、7900HT 系统、QuantStudio™ 12k Flex、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOne™、StepOnePlus™、ViiA™ 7 系统
反应次数500 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶
产品线RNA-to-CT™,SYBR™
产品类型一步法 qRT-PCR 试剂盒
纯度或质量等级UP (Ultra Pure)
数量500 次反应
逆转录酶ArrayScript™ UP
运行时间标准
样品类型RNA
运输条件湿冰
足够用于500 Reactions at 20 μL
检测方法SYBR
适用于(应用)基因表达
标签或染料SYBR Green
PCR 方法一步法 RT-qPCR
反应速度标准
Unit SizeEach
内容与储存
Sufficient for 500 reactions at 20 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.