TaqMan™ PreAmp 预混液
TaqMan™ PreAmp 预混液
引用和文献 (52)
Applied Biosystems™

TaqMan™ PreAmp 预混液

TaqMan™ PreAmp 预混液可预先扩增少量 cDNA，而不对样品引入扩增偏倚。预扩增使您能够将您的珍贵样品拉伸至更多的实时荧光定量 PCR 反应中。TaqMan™ PreAmp 预混液能让您：•分析激光捕获显微切割了解更多信息
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货号反应次数
439112840 次反应
4488593200 次反应
货号 4391128
价格（CNY)
13,733.00
Each
添加至购物车
反应次数:
40 次反应
价格（CNY)
13,733.00
Each
添加至购物车
TaqMan™ PreAmp 预混液可预先扩增少量 cDNA，而不对样品引入扩增偏倚。预扩增使您能够将您的珍贵样品拉伸至更多的实时荧光定量 PCR 反应中。

TaqMan™ PreAmp 预混液能让您：

•分析激光捕获显微切割、针刺活检和福尔马林固定石蜡包埋 (FFPE) 组织等珍贵样品的 mRNA
• 将低至 1 ng 的 cDNA 拉伸至200次实时荧光定量 PCR 反应中，以使用 TaqMan™ 基因表达测定试剂盒进行基因表达分析
• 用极少的手动操作时间分析多达100个基因表达靶标

保持靶标的平衡而无预扩增偏倚
过去，预扩增试剂盒和技术经常导致靶标的不均匀扩增和不正确或有偏倚的数据（表现为预扩增和未扩增样品之间的相关系数较低）。TaqMan™ PreAmp 预混液可实现无偏倚扩增，并在扩增和未扩增的 cDNA 之间提供极高的相关性。

简单工作流程
使用 TaqMan™ PreAmp 预混液，预扩增构建只需要几分钟。在将 TaqMan™ 测定一次性汇总后，预扩增构建（将汇总的测定与 cDNA 样品和 TaqMan™ PreAmp 预混液混合）只需要额外花费五分钟。使用 GeneAmp™ 9700 热循环仪进行循环需要1.5小时。
仅供科研使用。不可用于诊断程序。
规格
适用于（设备）7500 Fast 系统、7500 系统、7900HT 系统、QuantStudio™ 12k Flex、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOne™、StepOnePlus™、ViiA™ 7 系统
热启动内置热启动
反应次数40 次反应
参比荧光染料无 ROX
聚合酶AmpliTaq Gold DNA 聚合酶
产品线TaqMan
产品类型PreAmp 预混液
数量40 reactions
样品类型cDNA
足够用于40 次反应
容量1 mL
检测方法引物-探针
适用于（应用）预扩增
高 GC PCR 扩增效果高
PCR 方法qPCR
Unit SizeEach
内容与储存
含有 2X TaqMan™ PreAmp 预混液 (1 mL)。在 2–8°C 下储存。

保证的下限有效期为 60 天（确切的有效期已印在产品和 CofA 上）。

常见问题解答 (FAQ)

Can I use preamplification with the TaqMan SNP Genotyping Assays?

There is a preamplification protocol included with our TaqMan Sample-to-SNP Kit. This is designed to work with a lysate sample.

I want to purchase TaqMan Array Human MicroRNA A Cards v2.0 (Cat. No. 4398965). What other reagents do I need to purchase in addition to these cards?

In order to perform reverse transcription on these cards, you will need the following:

  • TaqMan MicroRNA Reverse Transcription Kit (Cat. No. 4366596)
  • Megaplex RT Primers pools (for human Type A cards Cat. No. 4399966, for Type B cards Cat. No. 4444281)
You can proceed with or without a preamplification step which depends on the total RNA amount. Total RNA amount of 1‐1000 ng supports reverse transcription reaction with preamplification, whereas 350‐1000 ng of total RNA supports a reaction without preamplification. In case you are performing preamplification you would also need the TaqMan PreAmp Master Mix (Cat. No. 4391128) and the Megaplex PreAmp Primers, Human Pool A v2.1 (Cat. No. 4399233). You will also need the Master Mix for the qPCR reaction. It is not included in the card kit. The recommended Master Mix is: TaqMan Fast Advanced Master Mix – run mode Fast (Cat. No. 4444556) For further information, please see the following link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

How does the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit differ from TaqMan PreAmp Master Mix?

TaqMan PreAmp Master Mix is designed for targeted preamplification of up to 100 targets from small amounts of cDNA, while SuperScript IV Single Cell/Low Input cDNA PreAmp Kit allows global preamplification of full-length cDNA from single cells or low input RNA. It provides reagents for cell lysis, reverse transcription and preamplification steps.

How do I reorder the TaqMan PreAmp Master Mix, Cat No. 4384266? It does not match the Cat. No. when I search online.

This is an internal part number for the TaqMan PreAmp Master Mix. To order, please use Cat No. 4391128 for the 40 reaction size or Cat. No. 4488593 for the 200 reaction size. This is the same product.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

引用和文献 (52)

引用和文献
Abstract
Target genes of neuron-restrictive silencer factor are abnormally up-regulated in human myotilinopathy.
Authors:Barrachina M, Moreno J, Juves S, Moreno D, Olive M, Ferrer I
Journal:Am J Pathol
PubMed ID:17823282
Myotilinopathy is a subgroup of myofibrillar myopathies caused by mutations in the myotilin gene in which there is aggregation of abnormal cytoskeletal proteins and ubiquitin. We report here on the accumulation of neuron-related proteins such as ubiquitin carboxy-terminal hydrolase L1 (UCHL1), synaptosomal-associated protein 25, synaptophysin, and alpha-internexin in aberrant protein ... More
Differential expression of microRNA-675, microRNA-139-3p and microRNA-335 in benign and malignant adrenocortical tumours.
Authors:Schmitz KJ, Helwig J, Bertram S, Sheu SY, Suttorp AC, Seggewiss J, Willscher E, Walz MK, Worm K, Schmid KW
Journal:J Clin Pathol
PubMed ID:21471143
'Background For the clinical management of adrenocortical neoplasms it is crucial to correctly distinguish between benign and malignant tumours. Even histomorphologically based scoring systems do not allow precise separation in single lesions, thus novel parameters are desired which offer a more accurate differentiation. The tremendous potential of microRNAs (miRNAs) as ... More
Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
Authors:Reed-Geaghan EG, Reed QW, Cramer PE, Landreth GE
Journal:J Neurosci
PubMed ID:21084593
'Alzheimer''s disease (AD) is characterized by the deposition of beta-amyloid (Abeta)-containing plaques within the brain that is accompanied by a robust microglial-mediated inflammatory response. This inflammatory response is reliant upon engagement of innate immune signaling pathways involving the toll-like receptors (TLRs). Studies assessing the roles of TLRs in AD pathogenesis ... More
Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells.
Authors:Sieuwerts AM, Kraan J, Bolt J, van der Spoel P, Elstrodt F, Schutte M, Martens JW, Gratama JW, Sleijfer S, Foekens JA
Journal:J Natl Cancer Inst
PubMed ID:19116383
'Identification of specific subtypes of circulating tumor cells in peripheral blood of cancer patients can provide information about the biology of metastasis and improve patient management. However, to be effective, the method used to identify circulating tumor cells must detect all tumor cell types. We investigated whether the five ... More
Altered GABAA receptor-mediated synaptic transmission disrupts the firing of gonadotropin-releasing hormone neurons in male mice under conditions that mimic steroid abuse.
Authors:Penatti CA, Davis MC, Porter DM, Henderson LP
Journal:J Neurosci
PubMed ID:20463213
'Gonadotropin-releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion. Self-administration of excessive doses of anabolic androgenic steroids (AAS) disrupts reproductive function and may have critical repercussions for pubertal onset in adolescent users. Here, we demonstrate that ... More
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